Mapping and DNA sequence characterisation of the Rysto locus conferring extreme virus resistance to potato cultivar ‘White Lady’

Kondrák, Mihály; Kopp, Andrea; Uri, Csilla; Sós-Hegedűs, Anita; Csákvári, Edina; Schiller, Mátyás; Barta, Endre; Cernák, István; Polgár, Zsolt; Taller, János; Bánfalvi, Zsófia

doi:10.1371/journal.pone.0224534

Cited by 6 publications

(9 citation statements)

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“…The variant Rysto-like26 found in cultivars Alicja, Bzura and White Lady, as well as the variant Rysto-like16 found in the breeding line PW363, encode a protein with 100% identity to the Ry sto reference protein. One of the two additional variants found in cultivar Alicja, Rysto-like30, showed 100% nucleotide identity to the TMV2 gene in cultivar White Lady, identi ed by ne-scale genetic mapping and characterized by Sanger sequencing in a previous study [25]. TMV2 displayed 95% identity to the reference Ry sto gene published by Grech-Baran et al 2020 [19].…”

Section: Presence Of Ry Sto Homologues In Potato Cultivarsmentioning

confidence: 82%

“…The possible reason might be different plant material used in this study (the tetraploid potato cultivar Alicja) and the previous study (the dihaploid clone Alicja) [19] or just sequencing errors, as such regions are prone to errors. Different Rysto-like variants identi ed in different cultivars tested, as well as additional variants identi ed in cultivar Alicja in this study, may re ect a different origin of the gene (Figure S2) [15,19,25]. The source of the Ry sto reference gene is the dihaploid clone Alicja, which was obtained from the tetraploid potato cultivar Alicja via parthenogenesis [19,26].…”

Section: Presence Of Ry Sto Homologues In Potato Cultivarsmentioning

confidence: 88%

“…In total, 298 genotypes representing 29 accessions of 26 tuber-bearing Solanum species from the National Centre for Plant Genetic Resources: Polish Genebank (IHAR-PIB, Radzików, Poland) (Table S2), H. niger (accession TN-82-763) and P. peruviana (accession TN-82-765) from the National Plant Gene Bank of Iran (Mahdasht Road, Karaj, 31359-33151, Iran) were used. Potato cultivars Alicja, Bzura, Hinga, Nimfy, White Lady and the potato breeding line PW363 known to carry the Ry sto gene, were used as resistant controls, while cultivars Irys, Irga, Nicola and Vineta without the Ry sto gene were used as susceptible controls [15,19,25]. A subset of tuber-bearing Solanum spp.…”

Section: Plant Materialsmentioning

confidence: 99%

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Diversity of the Ry sto gene conferring resistance to potato virus Y in wild relatives of potato

Paluchowska,

Rossmann,

Lysøe

et al. 2024

Preprint

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Background Potato virus Y (PVY) is among the economically most damaging viral pathogen in production of potato (Solanum tuberosum) worldwide. The gene Rysto derived from the wild potato relative Solanum stoloniferum confers extreme resistance to PVY. Results The presence of Rysto and diversity of it was investigated in wild relatives of potato (298 genotypes representing 29 accessions of 26 tuber-bearing Solanum species) using PacBio amplicon sequencing. A total of 55 unique Rysto-like sequences were identified in 72 genotypes representing 12 accessions of ten Solanum species and six resistant controls (potato cultivars Alicja, Bzura, Hinga, Nimfy, White Lady and breeding line PW363). The 55 Rysto-like sequences showed 89.87 to 99.98% nucleotide identity to the Rysto reference gene, and these encoded in total 45 unique protein sequences. While Rysto-like26 identified in Alicja, Bzura White Lady and Rysto-like16 in PW363 encode a protein identical to the Rysto reference, the remaining 44 predicted Rysto-like proteins were 65.93 to 99.92% identical to the reference. Higher levels of diversity of the Rysto-like sequences were found in the wild relatives of potato than in the resistant control cultivars. The TIR and NB-ARC domains were the most conserved within the Rysto-like proteins, while the LRR and C-JID domains were more variable. Several of the tested Solanum species, including S. antipoviczii and S. hougasii, showed resistance to PVY. This study demonstrated Hyoscyamus niger, a Solanaceae species distantly related to Solanum, as a host of PVY. Conclusions The new Rysto-like variants, as well as the PVY resistant potato genotypes identified in this study, could be potential resistance gene sources against PVY in potato breeding. Identification of H. niger as a host for PVY is important for cultivation of this plant, studies on the PVY management, its ecology, and migrations. The amplicon sequencing based on PacBio SMRT technology and the following data analysis pipeline described in our work may be applied to obtain the nucleotide sequences and analyze any full-length genes from any, even polyploid, organisms.

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Section: Presence Of Ry Sto Homologues In Potato Cultivarsmentioning

confidence: 82%

Section: Presence Of Ry Sto Homologues In Potato Cultivarsmentioning

confidence: 88%

Section: Plant Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Diversity of the Ry sto gene conferring resistance to potato virus Y in wild relatives of potato

Paluchowska,

Rossmann,

Lysøe

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The variant Rysto-like26 found in cultivars Alicja, Bzura and White Lady, as well as the variant Rysto-like16 found in the breeding line PW363, encode a protein identical to the Ry sto reference protein. One of the two additional variants found in cultivar Alicja, Rysto-like30 , showed 100% nucleotide identity to the TMV2 gene in cultivar White Lady, identified by fine-scale genetic mapping and characterized by Sanger sequencing in a previous study [ 27 ]. TMV2 displayed 95% identity to the reference Ry sto gene published by Grech-Baran et al 2020 [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of the Rysto gene conferring resistance to potato virus Y in wild relatives of potato

Paluchowska,

Lim Rossmann,

Lysøe

et al. 2024

BMC Plant Biol

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Background Potato virus Y (PVY) is among the economically most damaging viral pathogen in production of potato (Solanum tuberosum) worldwide. The gene Rysto derived from the wild potato relative Solanum stoloniferum confers extreme resistance to PVY. Results The presence and diversity of Rysto were investigated in wild relatives of potato (298 genotypes representing 29 accessions of 26 tuber-bearing Solanum species) using PacBio amplicon sequencing. A total of 55 unique Rysto-like sequences were identified in 72 genotypes representing 12 accessions of 10 Solanum species and six resistant controls (potato cultivars Alicja, Bzura, Hinga, Nimfy, White Lady and breeding line PW363). The 55 Rysto-like sequences showed 89.87 to 99.98% nucleotide identity to the Rysto reference gene, and these encoded in total 45 unique protein sequences. While Rysto-like26 identified in Alicja, Bzura, White Lady and Rysto-like16 in PW363 encode a protein identical to the Rysto reference, the remaining 44 predicted Rysto-like proteins were 65.93 to 99.92% identical to the reference. Higher levels of diversity of the Rysto-like sequences were found in the wild relatives of potato than in the resistant control cultivars. The TIR and NB-ARC domains were the most conserved within the Rysto-like proteins, while the LRR and C-JID domains were more variable. Several Solanum species, including S. antipoviczii and S. hougasii, showed resistance to PVY. This study demonstrated Hyoscyamus niger, a Solanaceae species distantly related to Solanum, as a host of PVY. Conclusions The new Rysto-like variants and the identified PVY resistant potato genotypes are potential resistance sources against PVY in potato breeding. Identification of H. niger as a host for PVY is important for cultivation of this plant, studies on the PVY management, its ecology, and migrations. The amplicon sequencing based on PacBio SMRT and the following data analysis pipeline described in our work may be applied to obtain the nucleotide sequences and analyze any full-length genes from any, even polyploid, organisms.

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“…Payette Russet has ‘extreme resistance’ governed by the NLR gene, Ry sto , which has been introgressed from the wild potato species, S. stoloniferum , into commercial cultivars [ 43 ]. The Ry sto locus has been mapped to the distal arm of potato chromosome 12, which contains four clusters of resistance genes [ 44 , 45 ]. An NLR protein encoded by the Ry sto gene recognizes and binds to the coat protein encoded by strains of PVY and PVA [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Small RNA Profiling of Potato Virus Y Infected Potato Cultivars, Including Systemically Infected Russet Burbank

Ross

Zidack

McDonald

et al. 2022

Viruses

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Potatoes are the world’s most produced non-grain crops and an important food source for billions of people. Potatoes are susceptible to numerous pathogens that reduce yield, including Potato virus Y (PVY). Genetic resistance to PVY is a sustainable way to limit yield and quality losses due to PVY infection. Potato cultivars vary in their susceptibility to PVY and include susceptible varieties such as Russet Burbank, and resistant varieties such as Payette Russet. Although the loci and genes associated with PVY-resistance have been identified, the genes and mechanisms involved in limiting PVY during the development of systemic infections have yet to be fully elucidated. To increase our understanding of PVY infection, potato antiviral responses, and resistance, we utilized RNA sequencing to characterize the transcriptomes of two potato cultivars. Since transcriptional responses associated with the extreme resistance response occur soon after PVY contact, we analyzed the transcriptome and small RNA profile of both the PVY-resistant Payette Russet cultivar and PVY-susceptible Russet Burbank cultivar 24 h post-inoculation. While hundreds of genes, including terpene synthase and protein kinase encoding genes, exhibited increased expression, the majority, including numerous genes involved in plant pathogen interactions, were downregulated. To gain greater understanding of the transcriptional changes that occur during the development of systemic PVY-infection, we analyzed Russet Burbank leaf samples one week and four weeks post-inoculation and identified similarities and differences, including higher expression of genes involved in chloroplast function, photosynthesis, and secondary metabolite production, and lower expression of defense response genes at those time points. Small RNA sequencing identified different populations of 21- and 24-nucleotide RNAs and revealed that the miRNA profiles in PVY-infected Russet Burbank plants were similar to those observed in other PVY-tolerant cultivars and that during systemic infection ~32% of the NLR-type disease resistance genes were targeted by 21-nt small RNAs. Analysis of alternative splicing in PVY-infected potato plants identified splice variants of several hundred genes, including isoforms that were more dominant in PVY-infected plants. The description of the PVYN-Wi-associated transcriptome and small RNA profiles of two potato cultivars described herein adds to the body of knowledge regarding differential outcomes of infection for specific PVY strain and host cultivar pairs, which will help further understanding of the mechanisms governing genetic resistance and/or virus-limiting responses in potato plants.

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Mapping and DNA sequence characterisation of the Rysto locus conferring extreme virus resistance to potato cultivar ‘White Lady’

Cited by 6 publications

References 50 publications

Diversity of the Ry sto gene conferring resistance to potato virus Y in wild relatives of potato

Diversity of the Ry sto gene conferring resistance to potato virus Y in wild relatives of potato

Diversity of the Rysto gene conferring resistance to potato virus Y in wild relatives of potato

Transcriptome and Small RNA Profiling of Potato Virus Y Infected Potato Cultivars, Including Systemically Infected Russet Burbank

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