Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.)

Abstract

Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.

Genome-Wide Association Study Identifies NBS-LRR-Encoding Genes Related with Anthracnose and Common Bacterial Blight in the Common Bean

“…Some NBS genes showed significant expression differences between resistant and susceptible lines, such as Phvul.004G008400 and Phvul.011G192400 for ANT disease and Phvul.002G129900 and Phvul.011G200900 for CBB disease. Differences between genotypes were also observed in other crops, such as Sr35 in wheat (Saintenac et al, 2013 ), Bol016084 in cabbage (Kim et al, 2015 ), and Phvul.001G243700 in the common bean (Chen et al, 2017 ). In addition, some genes were found to be highly induced in resistant lines after pathogen infection.…”

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

“…Alternatively, our material showed anthracnose resistance to race 2047 and resistance can easily be transferred to local commercially grown cultivars because these cultivars are adapted to the local environment and do not have limitations as those of G2333 and other differential cultivars. Over past few years, new anthracnose resistance genes were identified and mapped from diverse anthracnose resistant genotypes ( Gonçalves-Vidigal et al, 2011 ; Richard et al, 2014 ; Lacanallo and Gonçalves-Vidigal, 2015 ; Trabanco et al, 2015 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ). We expect our anthracnose resistant common bean material may contain diverse anthracnose resistance genes as the C. lindemuthianum virulence spectrum in NW region is different than the US, France and Canada ( Padder et al, 2017 ).…”

“…Seventeen of these genes are numbered from Co-1 to Co-17 , however many anthracnose resistance genes have alphabets as suffix such as Co-x , Co-y , Co-w , Co-Pa , Co-z , Co-1 HY , and are mapped to bean chromosomes where numbered genes are located ( Zuiderveen et al, 2016 ). Co-1 and its alleles, Co-14 , Co-x , Co-w , Co-1 HY , Co-1 65-X , Co-1 73-X , and Co-Pa anthracnose resistance genes are mapped to the distal end of bean chromosome Pv01 ( Melotto and Kelly, 2000 ; Geffroy et al, 2008 ; Gonçalves-Vidigal et al, 2011 ; Campa et al, 2014 ; Richard et al, 2014 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ). Five anthracnose resistance genes ( Co-u , CoPv02c 3-X , CoPv02c 7-X , CoPv02c 19-X , and CoPv0c 2449-X ) are mapped to Pv02 ( Kelly et al, 2003 ; Campa et al, 2014 ).…”

“…Genome wide association studies (GWAS) showed association of a SNP and SSR marker located at Pv10 with anthracnose resistance ( Zuiderveen et al, 2016 ; Wu et al, 2017 ). Access to P. vulgaris whole genome ( Schmutz et al, 2014 ; Vlasova et al, 2016 ) and SNP Bean Chips ( Hyten et al, 2010 ; Song et al, 2015 ) has resulted in the fine mapping of many economically important traits in beans including anthracnose resistance ( Richard et al, 2014 ; Burt et al, 2015 ; Gonzalez et al, 2015 ; Oblessuc et al, 2015 ; Padder et al, 2016 ; Zuiderveen et al, 2016 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ; Mungalu et al, 2020 ).…”

Morphological traits associated with anthracnose (Colletotrichum lindemuthianum) resistance in selected common bean (Phaseolus vulgaris L.) genotypes

“…Field losses may be up to 100% under climatic and soil conditions favourable to the disease (Bassanezi et al, 2001;Lopes and Berger, 2001;Paulert et al, 2009;Tullu et al, 2003). The production and the use of anthracnose resistant seeds is one control and management measure that is effective, safe and cheap in dealing with the disease (Chen et al, 2017).…”