Mapping and marker identification for sex-determining in the Pacific abalone, Haliotis discus hannai Ino

Sex determination mechanisms and sex control approaches in aquaculture animals

“…GSD with XX/XY or ZZ/ZW system has been widely identified in fish species, such as common carp (Cyprinus carpio) (Wu et al, 1990), yellow catfish (Pelteobagrus fulvidraco) (Liu et al, 2013b;Wang et al, 2009a), Ussuri catfish (Pseudobagrus ussuriensis) (Pan et al, 2015), Lanzhou catfish (Silurus lanzhouensis) (Wang et al, 2021), Nile tilapia (Oreochromis niloticus) (Li et al, 2015), Northern pike (Esox lucius) (Pan et al, 2019), half-smooth tongue sole (Cynoglossus semilaevis) (Chen et al, 2014;Cui et al, 2017), oyster pompano (Trachinotus anak) (Fan et al, 2021), and Amur sturgeon (Acipenser schrenckii) (Ruan et al, 2021) (Figure 1 and Table 1). GSD has also been detected in other aquaculture animals, including turtles (Montiel et al, 2016), frogs (Flament, 2016;Wallace et al, 1999;Yoshimoto et al, 2008), sea urchins (Lipani et al, 1996), sea cucumbers (Wei et al, 2021), crabs (Fang et al, 2020;Shi et al, 2018;Waiho et al, 2019), lobsters (Chandler et al, 2017), and shellfish (Guo and Allen, 1994;Luo et al, 2021) (Figure 1 and Table 1).…”

Gonadal Transcriptome Sequencing Analysis Reveals the Candidate Sex-Related Genes and Signaling Pathways in the East Asian Common Octopus, Octopus sinensis

“…Based on the transcriptome analysis, at least 21 DEGs involved in sex determination/differentiation were identified. These genes included Dmrt1, Foxn5, Foxj1, Sox14, Sox30, Fem1, Tssk6, Tssk1, Tssk3 and other potential candidates that have been reported previously in vertebrates and in mollusks [21][22][23][24][25][26][27].…”

“…Currently, a number of sex-related genes have been identified from diverse mollusks based on a gonadal transcriptome analysis. These include transcription factor Sox2 (Sox2), forkhead box protein Z (Foxz), heat shock transcription factor, Y-linked (Hsfy), forkhead box protein L2 (Foxl2) and transcription factor HES-1 (Hes1) in dwarf surf clam (M. lateralis) [21]; Foxl2, beta-catenin (β-catenin) and sex-lethal (Sxl) in blood clam (Tegillarca granosa) [22]; FoxL2 and doublesex-and mab-3-related transcription factor A2 (Dmrta2) in Pacific abalone (Haliotis discus hannai) [23]; transcription factor SoxH (SoxH), FoxL2, doublesex (Dsx), feminization (Fem), GATA binding protein 4 (Gata4), wnt family member 4 (Wnt4) and β-catenin in Pacific oyster (C. gigas) [24,25]; testis-specific serine/threonine-protein kinase 1 (Tssk1), testis-specific serine/threonine-protein kinase 4 (Tssk4), testis-specific serine/threonine-protein kinase 5 (Tssk5), doublesex-and mab-3-related transcription factor 1 (Dmrt1), sperm protein 17 (Sp17) and feminization-1 (Fem1) in fluted giant clam (Tridacna squamosa) [26]; DmrtA2, transcription factor Sox9 (Sox9), Fem-1b, Fem-1c, vitellogenin (Vg), cytochrome P450 family 17 subfamily A member 1 (Cyp17a1) and spermatogenesis-and oogenesis-specific basic Helix-Loop-Helix 2 (Sohlh2) in razor clam (Sinonovacula constricta) [27]; and Dmrt1, transcription factor Sox30 (Sox30), testis-specific serine/threonine-protein kinase (Tssk), Gata1 and Vg in yesso scallop (Patinopecten yessoensis) [17]. The sex-determination genes in vertebrates, such as Foxl2, Dmrt1 and sry, were also identified in mollusks, which suggests that the sex-determination mechanisms in animals may be conserved.…”