Mapping Cd2+-induced membrane permeability changes of single live cells by means of scanning electrochemical microscopy

Filice, Fraser P.; Li, Michelle S. M.; Henderson, Jeffrey D.; Ding, Zhifeng

doi:10.1016/j.aca.2015.12.027

Cited by 39 publications

(35 citation statements)

References 60 publications

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“…Experimental PACs were extracted to the tallest (or largest) point of the cell's electrochemical feedback to provide higher accuracy in its quantification (smaller tip‐to‐sample distance). Additionally, since the membrane permeability of cells was previously demonstrated to be consistent across the entire cell using a 10 μm Pt UME (RG3), PACs extracted with slight deviations from the center of the cell did not affect its membrane permeability coefficient.…”

Section: Resultsmentioning

confidence: 99%

“…Ultimately with higher (acute) exposure (500–1000 μM), the permeability coefficients increased and returned to values similar to that of control cells. This trend agrees well with another study where the T24 cells were treated with Cd (II) as the toxic metal . However, the response triggered by both Cr (VI) and Cd (II) were found to differ in the concentrations required to initiate each permeability region.…”

Section: Discussionmentioning

confidence: 99%

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Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Henderson

Filice

et al. 2017

ChemElectroChem

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The effects of exposure to toxic heavy metals, such as chromium, are of interest in scientific research, owing to its association with oxidative stress, cytotoxicity, and carcinogenicity. This study aims to explore the effects of Cr (VI) on live cell responses. Herein, scanning electrochemical microscopy (SECM) is employed by using depth scan imaging and feedback mode to monitor the membrane permeability of single live human bladder cancer (T24) cells following 1 h incubations with Cr (VI) stimuli. By using membrane‐permeable and impermeable redox mediators, ferrocenemethanol and ferrocenecarboxylic acid, respectively, SECM depth scans yield both electrochemical and topographic information. This provides insights into the relative changes in membrane homeostasis with increased exposure to Cr (VI). Here, SECM has shown great power in determining membrane response to Cr (VI) exposure. Dependent on the level of exposure, transition between three distinct trends was observed. At low incubation concentrations of Cr (VI), the cell membrane permeability coefficients were relatively unaffected. With moderate increases in Cr (VI) concentrations, membrane permeability coefficients of the incubated cells were observed to decrease. Finally, with the higher incubation concentrations, membrane permeability coefficients were found to increase toward values similar to control cells. The Cr (VI) toxicity was further investigated by means of a MTT cell viability study, which exhibited a similar decreasing trend to the cell membrane permeability. These findings further demonstrate the strength of SECM as a bioanalytical technique for monitoring cellular homeostasis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Henderson

Filice

et al. 2017

ChemElectroChem

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“…A detailed description of the SECM instrumentation and experimental procedures can be found elsewhere . Briefly, SECM experiments were carried out using the piezoelectric positioning system of a modified Alpha‐SNOM (WITec, Ulm, Germany).…”

Section: Methodsmentioning

confidence: 99%

Simulation Assisted Nanoscale Imaging of Single Live Cells with Scanning Electrochemical Microscopy

Filice

Ding

2018

Advcd Theory and Sims

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Nanoelectrodes have become an area of significant interest in recent years, which provide a number of advantages for imaging with scanning electrochemical microscopy (SECM). Since the resolution of SECM imaging is directly dependent on the size of the electrode probe, the reduced surface area of nanoelectrodes allows for the imaging of smaller sample features, or more localized electrochemical reactivity. Nanoelectrodes with a radius of 130 nm are employed to image the surface of single live cells. The use of nanoscale imaging, however, introduces additional complexity into the simulation modeling of the cell surface geometry and electrochemical reactivity. The creation of tailored simulation models accounting for these specific physical conditions is utilized to overcome the additional challenges to the characterization of the electrochemical system. Methodologies for the experimental mapping and creation of 3D simulation models of single live cells have been well developed, which are presented herein. These developments include characterization of cell surface topography, tip-to-cell distance, as well as cell membrane permeability quantification. The advanced quantification of the complex nanoscale imaging of single live cells assisted by theoretical simulations provides increased versatility to SECM as an already powerful bioanalytical tool.

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“…To quantify the membrane permeability coefficients of these hydrophilic redox mediators under the treatment of Cd 2+ , the authors established a simulation model in 2D axial symmetry using COMSOL Multiphysics. The model geometry, including the tip size and the shape of the electrolyte cell, were redesigned to be representative of the physical dimensions of all of the elements in this biological SECM system [153,154]. In this model, the approach curves showed less negative feedback characteristics with an increasing permeability coefficient from 0 to 1.0 × 10 − 3 m/s.…”

Section: Applicationsmentioning

confidence: 99%

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

Huang

Lou

et al. 2018

Materials

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Scanning electrochemical microscopy (SECM) is a chemical microscopy technique with high spatial resolution for imaging sample topography and mapping specific chemical species in liquid environments. With the development of smaller, more sensitive ultramicroelectrodes (UMEs) and more precise computer-controlled measurements, SECM has been widely used to study biological systems over the past three decades. Recent methodological breakthroughs have popularized SECM as a tool for investigating molecular-level chemical reactions. The most common applications include monitoring and analyzing the biological processes associated with enzymatic activity and DNA, and the physiological activity of living cells and other microorganisms. The present article first introduces the basic principles of SECM, followed by an updated review of the applications of SECM in biological studies on enzymes, DNA, proteins, and living cells. Particularly, the potential of SECM for investigating bacterial and biofilm activities is discussed.

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Mapping Cd2+-induced membrane permeability changes of single live cells by means of scanning electrochemical microscopy

Cited by 39 publications

References 60 publications

Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Simulation Assisted Nanoscale Imaging of Single Live Cells with Scanning Electrochemical Microscopy

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

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