Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Cai, Zhen; Sheng, Yujun; Zhang, L.-F.; Wang, Y.; Jiang, Xiaoling; Lv, Zhi-yue; Xu, Ning

doi:10.4238/vol10-3gmr1113

Cited by 4 publications

(5 citation statements)

References 27 publications

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“…NES PCATS exhibited good viability over at least 63 h in culture, which is in accordance with the viability reported for precision-cut slices of other tissues (Thiele et al, 2017). Most of the publications on adipose tissue slices report a good viability up to 24 h of culture (Mersmann and Shparber, 1993;Ramsay and Rosebrough, 2005;Cai et al, 2011). Only two of them cultured thin adipose tissue slices for longer periods, i.e., five (Anayama et al, 2015) and 14 days (Schopow et al, 2020).…”

Section: Discussionsupporting

confidence: 85%

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

et al. 2020

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Adipose tissue plays key roles in energy homeostasis. Understanding its metabolism and regulation is essential to predict the impact of environmental changes on wildlife health, especially in fasting-adapted species. However, in vivo experimental work in wild vertebrates can be challenging. We have developed a novel in vitro approach of precision-cut adipose tissue slices from northern elephant seal (Mirounga angustirostris) as a complementary approach to whole animal models. Blubber biopsies were collected from 14 pups during early and late post-weaning fast (Año Nuevo, CA, United States), precision-cut into 1 mm thick slices and maintained in culture at 37°C for at least 63 h. The slices exhibited an efficient response to ß-adrenergic stimulation, even after 2 days of culture, revealing good in vitro tissue function. The response to lipolytic stimulus did not vary between regions of outer and inner blubber, but was higher at early than at late fast for inner blubber slices. At early fast, lipolysis significantly reduced leptin production. At this stage, inner blubber slices were also more efficient at producing leptin than outer blubber slices, especially in the non-lipolytic condition. This model will aid the study of adipose tissue metabolism and its response to environmental stressors in marine mammals.

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Section: Discussionsupporting

confidence: 85%

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

et al. 2020

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“…49 The TRα isoform is predominantly expressed in adipocytes and mediates actions of thyroid hormone in these cells. 50 Thus, up-regulation of TRs could reduce concentrations of THs. TSH enhances the ability of the thyroid gland to trap iodide.…”

Section: ■ Discussionmentioning

confidence: 80%

“…TRs including TRα and TRβ can bind to specific DNA sequences (thyroid hormone response elements) on promoters to regulate target genes, which are involved in resistance to thyroid hormone (TRH) . The TRα isoform is predominantly expressed in adipocytes and mediates actions of thyroid hormone in these cells . Thus, up-regulation of TRs could reduce concentrations of THs.…”

Section: Discussionmentioning

confidence: 99%

Accumulation and Biotransformation of BDE-47 by Zebrafish Larvae and Teratogenicity and Expression of Genes along the Hypothalamus–Pituitary–Thyroid Axis

Zheng

Zhu

Liu

et al. 2012

Environ. Sci. Technol.

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Accumulation and effects of BDE-47 and two analogues, 6-OH-BDE-47 and 6-MeO-BDE-47, on ontogeny and profiles of transcription of genes along the hypothalamus-pituitary-thyroid (HPT) axis of zebrafish (Danio rerio) embryos exposed from 4 h post fertilization (hpf) to 120 hpf were investigated. The 96 h-LC(50) of the most toxic compound, based on teratogenicity, was 330 μg of 6-OH-BDE-47/L. 6-OH-BDE-47 significantly down-regulated expression of mRNA of thyroid stimulating hormone receptor (TSHR), thyroid hormone receptors (TRs, including TRα and TRβ), sodium/iodide symporter (NIS), and transthyretin (TTR) while up-regulating expression of thyroglobulin (TG) and thyrotropin-releasing hormone (TRH). Spontaneous movement was affected by 1 mg of 6-OH-BDE-47/L or 5 mg of 6-MeO-BDE-47/L. BDE-47 did not alter activity of larvae at any concentration tested. 6-MeO-BDE-47 significantly up-regulated expression of mRNA of TRH, TRα, TRβ and NIS. Both 6-OH-BDE-47 and 6-MeO-BDE-47 affected the thyroid hormone pathway. BDE-47 and 6-MeO-BDE-47 were accumulated more than 6-OH-BDE-47. 6-MeO-BDE-47 was transformed into 6-OH-BDE-47, but BDE-47 was not transformed into it. In summary, the synthetic brominated flame retardant, BDE-47, did not elicit the adverse effects caused by the other two analogues and appeared to have less toxicological relevance than the two natural product analogues 6-OH- and 6-MeO-BDE-47.

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“…Another mechanism is that PBDEs may deactivate the iodothyronine deiodinases (IDs: including three types, IDI, IDII and IDIII), and finally affect the metabolism and production of TH (Gereben et al, 2008;Butt et al, 2011;Roberts et al, 2015). The third mechanism is the direct binding of PBDEs to TH receptors (including thyroid hormone receptor α and β), which may not only affect the function of TH, but also change the levels of TH (Kitamura et al, 2008;Cai et al, 2011). These complex mechanisms may disturb the equilibrium of TH in serum simultaneously and lead to nonmonotonic relationships.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Association between serum polybrominated diphenyl ethers, new flame retardants and thyroid hormone levels for school students near a petrochemical complex, South China

et al. 2018

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As surrogates of polybrominated diphenyl ethers (PBDEs), new flame retardants (NFRs) include a series of chlorinated and brominated flame retardants. Though the NFRs are thought to induce similar thyroid hormone (TH) disrupting effects as PBDEs, few studies have focused on them. Given the increasing levels of NFRs in the environment, more in depth investigation of the potential TH disrupting effects of NFRs is warranted. This research involved a health survey to collect data and examine the associations between PBDEs, NFRs and TH. 174 school students lived near a petrochemical complex in South China participated in the survey, completing questionnaires and providing blood samples. Thirteen congeners of PBDEs, eight species of NFRs, TH and thyroid-stimulating hormone (TSH) were measured. The median levels of ΣPBDE (sum of thirteen congeners of PBDEs) and ΣNFR (sum of eight species of NFRs) for students were 140 and 240 ng g lipid, respectively. Nonmonotonic relationships were observed between quartile levels of PBDEs, NFRs and corresponding TH. In contrast to ΣPBDE that was positively associated with triidothyrine (T) level, ΣNFR was not statistically associated with TH. ΣPBDE + NFR (sum of thirteen congeners of PBDEs and eight species of NFRs) was significantly associated with T level.

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Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Cited by 4 publications

References 27 publications

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

Accumulation and Biotransformation of BDE-47 by Zebrafish Larvae and Teratogenicity and Expression of Genes along the Hypothalamus–Pituitary–Thyroid Axis

Association between serum polybrominated diphenyl ethers, new flame retardants and thyroid hormone levels for school students near a petrochemical complex, South China

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