Economic globalization promotes the economic development of underdeveloped regions but also influences the ecological environments of these regions, such as natural forest degradation. For inland developing regions with underdeveloped traffic routes, are the effects on the ecological environment also as obvious? To reveal the response characteristics of the ecological environment of the inland developing countries to globalization, we took Laos as an example, and used the land use/cover change data and also its exports and imports data to analyze the ecological environment change since the millennium. Land use transfer matrix analysis showed that Laos had encountered a large conversion of 14.43% natural forest to plantation forest since 2000 to 2017, and also a degradation of 5.94% natural forest to shrubland and grassland. Landscape pattern analysis showed that these changes were the main reasons of the fragmentation of ecological patches, which would lead to a reduction in biodiversity. More, topographic analysis further showed that natural forest degradation mainly took place in high-altitude and large slope areas, which could increase the potential of natural hazards such as floods. Coupling analysis with its exports and imports data indicated that economic globalization still had a significant impact on the country’s ecological environment although Laos is an inland developing country. Laos should strengthen the regulation of renewable resources such as forests and water resources, to avoid losing the renewable resources market while still enjoying the dividends of economic globalization. At the same time, it is necessary to accurately evaluate the indirect impacts of development on neighboring countries to ensure sustainable development.