Mapping genes essential for embryo development in Arabidopsis thaliana

Patton, David A.; Franzmann, Linda H.; Meinke, David W.

doi:10.1007/bf00273921

Cited by 65 publications

(50 citation statements)

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“…Promoter trapping has the further advantage over other mutagenic treatments in that it does not rely necessarily on the identification of mutant phenotypes (which may be low-frequency events) fop successful gene tagging, since the generation of functional gene fusions in situ allows the detection of tagged regulatory elements at high frequency (Koncz et al, 1989;. To date, however, no embryonic genes have been successfully isolated by the use of T-DNA tags (Errampalli et al, 1991;Meinke, 1992;Patton et al, 1991). …”

Section: Discussionmentioning

confidence: 99%

Identification of molecular markers of embryogenesis in Arabidopsis thaliana by promoter trapping

et al. 1994

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SummaryThe technique of promoter trapping has been exploited to identify markers of embryogenesis in Arabidopsis thallana. A population of transgenic A. thallana was generated containing the promoter trap vector pAgusBin19, following Agrobacferium tumefaciens-medlated transformation. This vector contains, at the T-DNA left border, a promoterless gusA gene, which is activeted following integration downstream of a native gene promoter that directs transgene transcription. Screening of a population of 430 independent transgenic lines revealed that 74 lines (17.2%) exhibited GUS activity in siliques, as determined by fluorimetric amy. Hietochemical GUS analysis was used to identify lines that expressed GUS in embryos, and three lines that were demonstrated to contain single T-DNA Inserts were analysed in detail. Each line showed a distinct pattern of GUS fusion activity. Fusion transcripts were identified, demonstrating that transcription was initiated in the genomic DNA flanking the T-DNA left border. Inverse PCR was used to clone the T-DNA flanking sequences, and for one line a corresponding cDNA was Identified, demonetrating that the tagged sequences are transcribed. The markers represent the earliest embryonic genes known to be expressed in plants.

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Section: Discussionmentioning

confidence: 99%

Identification of molecular markers of embryogenesis in Arabidopsis thaliana by promoter trapping

et al. 1994

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“…This location is shown on the updated genetic map available through the World Wide Web (http://mutant.lse.okstate.edu/). The bio1 locus described previously maps to position 74 cM on chromosome 5 (Patton et al, 1991;Franzmann et al, 1995). Only a single bio2 allele has been identified to date.…”

Section: The Bio2 Auxotroph Is An Embryo-defective Mutantmentioning

confidence: 99%

An Embryo-Defective Mutant of Arabidopsis Disrupted in the Final Step of Biotin Synthesis

et al. 1998

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Auxotrophic mutants have played an important role in the genetic dissection of biosynthetic pathways in microorganisms. Equivalent mutants have been more difficult to identify in plants. The bio1 auxotroph of Arabidopsis thaliana was shown previously to be defective in the synthesis of the biotin precursor 7,8-diaminopelargonic acid. A second biotin auxotroph of A. thaliana has now been identified. Arrested embryos from this bio2 mutant are defective in the final step of biotin synthesis, the conversion of dethiobiotin to biotin. This enzymatic reaction, catalyzed by the bioB product (biotin synthase) in Escherichia coli, has been studied extensively in plants and bacteria because it involves the unusual addition of sulfur to form a thiophene ring. Three lines of evidence indicate that bio2 is defective in biotin synthase production: mutant embryos are rescued by biotin but not dethiobiotin, the mutant allele maps to the same chromosomal location as the cloned biotin synthase gene, and gel-blot hybridizations and polymerase chain reaction amplifications revealed that homozygous mutant plants contain a deletion spanning the entire BIO2-coding region. Here we describe how the isolation and characterization of this null allele have provided valuable insights into biotin synthesis, auxotrophy, and gene redundancy in plants.

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“…Genetic evidence for intracellular trafficking of PIN proteins came with the analysis of the Arabidopsis mutant gnom (also called emb30) isolated in a screen for defects in early apical-basal patterning (Mayer et al, 1991;Patton et al 1991). gnom mutants show a variety of developmental defects.…”

Section: Constitutive Endocytic Cycling Of Pin Proteinsmentioning

confidence: 99%