Mapping genes governing flower architecture and pollen development in a double mutant population of carrot

Abstract

A linkage map of carrot (Daucus carota L.) was developed in order to study reproductive traits. The F2 mapping population derived from an initial cross between a yellow leaf (yel) chlorophyll mutant and a compressed lamina (cola) mutant with unique flower defects of the sporophytic parts of male and female organs. The genetic map has a total length of 781 cM and included 285 loci. The length of the nine linkage groups (LGs) ranged between 65 and 145 cM. All LGs have been anchored to the reference map. The objective of this study was the generation of a well-saturated linkage map of D. carota. Mapping of the cola-locus associated with flower development and fertility was successfully demonstrated. Two MADS-box genes (DcMADS3, DcMADS5) with prominent roles in flowering and reproduction as well as three additional genes (DcAOX2a, DcAOX2b, DcCHS2) with further importance for male reproduction were assigned to different loci that did not co-segregate with the cola-locus.

The carrot monoterpene synthase gene cluster on chromosome 4 harbours genes encoding flavour-associated sabinene synthases

“…Two carrot mutants, cola and yellow , were involved in the experiments. The cola mutant possesses dwarfish morphology, whereas reduced amount of chlorophyll resulted in yellowish leaves of yellow mutant 54 . The F 2 mapping population (VOM14) of 320 individuals was developed from an initial cross of a homozygous recessive yellow mutant as a female parent and a homozygous recessive cola mutant as pollen parent 54 , 55 .…”

High-throughput simple sequence repeat (SSR) mining saturates the carrot (Daucus carota L.) genome with chromosome-anchored markers

“…Ma et al [27] investigated the molecular genetic relationships among Chinese and Western orange carrot accessions, and Mandel et al [28] deduced the patterns of gene flow between cultivated and wild forms of carrot. These available SSR loci were also utilized for mapping anthocyanin biosynthesis genes [29], genes associated with flower architecture and fertility [30], and QTLs for nematode resistance [31]. Yet, the pool of available SSR markers is insufficient to cover the carrot genome with high resolution, and the currently available marker loci have not yet been anchored to their chromosomal locations.…”

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

“…In soybean, 25 nuclear genes affecting chlorophyll-deficiency have been identified and mapped [9]. Leaf-color mutants have also been reported in tomato [14], pepper [15], and carrots [16,17]. Investigation of these color mutants has contributed significantly to our understanding of chlorophyll metabolism and chloroplast development [18].…”