Mapping Genetic Locus Linked to Brown Planthopper Resistance in Rice Oryza sativa L.

Santhanalakshmi, S.; Saikumar, Surapaneni; Rao, Shubhada; Saiharini, A.; Khera, Pawan; Shashidhar, H. E.; Kadirvel, P.

doi:10.3923/ijpbg.2010.13.22

Cited by 20 publications

(19 citation statements)

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“…Berdasarkan studi literatur, diketahui bahwa genotipe PTB-33 merupakan salah satu kultivar tahan wereng cokelat dengan beberapa gen tahan wereng cokelat (Bph or bph; Jairin et al, 2007a;Sun et al, 2005;Nugaliyadde et al, 2004;Santhanalakhsmi et al, 2010). PTB-33 merupakan kultivar introduksi dari India yang memiliki gen ketahanan terhadap wereng cokelat, yaitu bph2, Bph3, bph4 yang terdapat pada kromosom 6, serta beberapa gen ketahanan lainnya yang diduga merupakan QTL (quantitative trait loci; Santhanalakshmi et al, 2010). Gen-gen bph inilah yang direncanakan untuk diintrogresikan ke dalam padi kultivar unggul Indonesia (IR-64, Pandan Wangi, Ciherang, dan Sintanur) sehingga akan diperoleh galur-galur padi harapan yang memiliki ketahanan terhadap wereng cokelat.…”

Section: Pendahuluanunclassified

“…Marka RM586 dan RM589 diketahui terpaut dengan gen Bph3 dan bph4 pada kromosom 6 (Gambar 6). Hasil penelitian yang telah dilakukan menunjukkan bahwa gen Bph3 dan bph4 telah diteliti dimiliki oleh kultivar PTB-33 (Jairin et al, 2007a;Santhanalakshmi et al, 2010) Hasil yang berbeda diperlihatkan oleh RM5953, dimana tidak terdapat adanya polimorphisme antara kultivar yang diuji, padahal RM5953 juga diketahui terpaut dengan gen yang sama dengan RM8213, yaitu gen Qbph4 dan Bph17(t) (Gambar 6). Semua kultivar terlihat memiliki pita DNA dengan ukuran 129bp sesuai dengan PCR product size yang diharapkan (Gambar 6).…”

Section: Marker Screening (Skrining Marka)unclassified

“…Adanya kedua gen tersebut, ditambah beberapa gen bph yang telah diketahui sebelumnya pada kultivar PTB-33 seperti Bph3 dan bph4 (Jairin et al, 2007a;Santhanalakshmi et al, 2010), menjadi salah satu alasan ketahanan yang tinggi pada kultivar ini terhadap berbagai biotipe wereng cokelat. Maqbool et al, (2001) menyatakan bahwa adanya beberapa gen ketahanan dalam satu kultivar diketahui dapat membentuk ketahanan yang tinggi pada tanaman.…”

Section: Marker Screening (Skrining Marka)unclassified

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Analisis Karakter Penting dan Skrining Marka-marka SSR yang Berasosiasi dengan Gen Ketahanan terhadap Wereng Coklat pada Lima Kultivar Padi

Prayoga

Dono²,

Yuwariah³

et al. 2018

zuriat

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Brown planthopper (Nilaparvata lugens Stal) is well-known as the major pest in rice cultivation in Indonesia and other countries in Asia. Developing resistant rice lines is the ideal option for economic and effective management. The experiments were aimed to obtain the important traits between Brown planthopper (BPH) resistant and susceptible genotypes, to obtain SSR markers that can be used in Marker Assisted Selection (MAS) and association of SSR markers with important traits. In this study, characterization of important traits and screening of eight SSR markers were performed for five genotypes i.e. PTB-33 (resistant genotype), IR-64 (tolerant), Pandan Wangi (susceptible), Ciherang (susceptible), and Sintanur (susceptible). PTB-33 showed higher photosynthetic rate (tolerant ability), longer trichomes size (antixenosis ability), and lower protein content (antibiosis ability) compared with other cultivars. RM8213 exhibited visible polymorphic bands between PTB-33 and other cultivars, meanwhile RM586 and RM589 showed polymorphic bands between PTB-33 and other cultivars, except with cv. IR-64. Z-mantel test for correlation between physiological traits and SSR markers showed that Qbph4 and Bph17(t) genes highly correlated with photosynthetic rates and trichomes length, whereas Bph3 and bph4 genes showed a high level of correlation with protein content.Abstrak : Hama wereng cokelat telah lama menjadi masalah utama dalam budidaya padi di Indonesia dan beberapa negara Asia lainnya. Merakit tanaman yang memiliki ketahanan terhadap hama ini, dipandang sebagai pendekatan yang lebih efektif dan ramah terhadap lingkungan dan manusia. Penelitian ini bertujuan untuk memperoleh perbedaan pada karakter penting padi antara kaultivar padi tahan dengan kultivar rentan wereng cokelat, selain itu juga untuk mendapatkan marka-marka SSR yang bisa digunakan untuk Marker Assisted Selection (MAS) dan hubungannya dengan karakter penting. Penelitian ini meliputi upaya pengamatan karakter penting dan skrining delapan marka SSR yang diduga berkaitan dengan gen bph (ketahanan terhadap wereng coklat) pada lima kultivar padi tetua yaitu PTB-33 (genotipe tahan), IR-64, Pandan Wangi, Ciherang, dan Sintanur (kultivar lokal). Pengujian karakter penting pada kultivar PTB-33 memiliki laju fotosintesis yang lebih tinggi (toleran), trikoma yang lebih panjang (antixenosis), dan kandungan protein yang lebih rendah (antibiosis) dibandingkan kultivar lainnya yang diuji. Hasil visualisasi pada delapan marka yang memperlihatkan bahwa RM8213 menunjukkan adanya polimorphisme pita DNA pada genotipe tahan PTB-33 dengan kultivar lainnya. Selain itu, marka RM586 dan RM589 juga menunjukkan adanya polimorphisme pada kultivar PTB-33 dengan kultivar lainnya kecuali dengan kultivar IR-64. Hasil analisis Z-Mantel antara marka SSR dengan karakter karakter penting tetua menunjukkan bahwa gen Qbph4 dan Bph17(t) mempunyai korelasi yang sangat tinggi dengan karakter laju fotosintesis dan panjang trikoma, sedangkan gen gen Bph3 dan bph4 berkorelasi tinggi dengan kandungan pr...

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Section: Pendahuluanunclassified

Section: Marker Screening (Skrining Marka)unclassified

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Analisis Karakter Penting dan Skrining Marka-marka SSR yang Berasosiasi dengan Gen Ketahanan terhadap Wereng Coklat pada Lima Kultivar Padi

Prayoga

Dono²,

Yuwariah³

et al. 2018

zuriat

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“…It has been used extensively in breeding programs aimed at increasing rice resistance to biotic stresses (Khush and Virk, 2005). The genetics of resistance in PTB33 is still not fully understood, but is likely governed by a number of major resistance genes (including bph2 and Bph3: Angeles et al 1986) and several minor genes (Santhanalakshmi et al 2010). IR62 is a modern rice variety released by IRRI in 1984.…”

Section: Plant Materialsmentioning

confidence: 99%

Adaptation of the brown planthopper,Nilaparvata lugens(Stål), to resistant rice varieties

Ferrater¹

2016

2016 International Congress of Entomology

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for the physical assistance in the laboratory, in the greenhouse and for the friendships which sprung from always working together. Alberto Naredo, through his extensive experience has patiently shared to me his knowledge in the brown planthopper life history and handling, techniques that are not even written in books. The jokes, laughter and fun interlaced in everyday greenhouse life were truly memorable. Bong Villareal and Jo Catindig, my bestfriends at IRRI who were not afraid to immediately correct my mistakes but readily praise my achievements. For the enumerable assistance in demonstrating entomology concepts that are comprehensive enough for me who came from a non-entomology background. For teaching me generously the most efficient way to do things in the laboratory and greenhouse, saving me some time and effort to do trial and error to figure out how to do things. Above all, the overflowing friendship and the sympathy that are readily available in whatever I go through. I cannot imagine the future without you! Carmencita C. Bernal, my-mom‖ at IRRI. The witty and funny remarks out of nowhere are always my anti-depressants. The infinite technical consultations and the fun travels and escapades together. For instilling in me that life is too short, so we should make the most out of every moment of our existence. Ma. Liberty P. Almazan, one of the sharpest persons I have encountered. A source of calm amid the storm of conducting greenhouse setups. My presentation editor before any talk or conference.

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“…o meet the demand of increasing population and maintain selfsufficiency the present rice production needs to be increased by 30% by the year 2020 (Singh et al, 2012). The task is quite challenging and the options available are very limited in view of plateauing trend of yield in high productivity areas, decreasing and degrading land and scarcity of water and labour (Santhanalakshmi et al, 2010). Rice (Oryza sativa L.) is one of the major crop species in which hybrids are used commercially (Selvaraj et al, 2011).…”

mentioning

confidence: 99%

Characterization of Some Rice Genotypes for Fertility Restoring Genes Using Rapd and SSR Markers

El-Banna

Kattab

Farid

2015

Egyptian Journal of Genetics and Cytology

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Mapping Genetic Locus Linked to Brown Planthopper Resistance in Rice Oryza sativa L.

Cited by 20 publications

References 1 publication

Analisis Karakter Penting dan Skrining Marka-marka SSR yang Berasosiasi dengan Gen Ketahanan terhadap Wereng Coklat pada Lima Kultivar Padi

Analisis Karakter Penting dan Skrining Marka-marka SSR yang Berasosiasi dengan Gen Ketahanan terhadap Wereng Coklat pada Lima Kultivar Padi

Adaptation of the brown planthopper,Nilaparvata lugens(Stål), to resistant rice varieties

Characterization of Some Rice Genotypes for Fertility Restoring Genes Using Rapd and SSR Markers

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