Mapping Helper Virus Functions for Cucumber Mosaic Virus Satellite RNA with Pseudorecombinants Derived from Cucumber Mosaic and Tomato Aspermy Viruses

Moriones, Enrique; López‐Díaz, Isabel; Fernández-Cuartero, B; Fraile, Aurora; Burgyán, József; García‐Arenal, Fernando

doi:10.1006/viro.1994.1682

Cited by 8 publications

(5 citation statements)

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“…Thus, the low accumulation of BSL6 in the inoculated leaves could be due to inefficiency in spreading in plants rather than the inefficiency of satBaMV replication or encapsidation. Such defects by sat RNA in long-distance movement have been previously noted for Ix-sat RNA associated with cucumber mosaic virus when tomato aspermy virus acts as a helper virus (Moriones et al 1992(Moriones et al , 1994Bernal and Garcia-Arenal 1994). How BSL6 satBaMV interfered with genomic RNA accumulation and attenuated the virus-induced symptoms in the upper noninoculated leaves remains to be determined.…”

Section: Discussionmentioning

confidence: 80%

Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants

Hsu¹,

Lee²,

Liu³

et al. 1998

MPMI

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Bamboo mosaic potexvirus (BaMV) supports the replication and encapsidation of a single-stranded satellite RNA (satBaMV). Full-length infectious cDNA clones of sat-BaMV variants with a 0.7 to 7.5% difference in nucleotide sequence were constructed, and in vitro transcripts were co-inoculated with BaMV RNA to barley protoplasts. Co-inoculation with BSL6 satBaMV transcripts resulted in a greatly reduced accumulation of genomic and subgenomic RNAs whereas co-inoculation with the prototype BSF4 or other variants did not. satBaMV variant BSL6-mediated interference occurred at the genomic RNA, subgenomic RNA, and capsid protein levels whereas the synthesis of minus strand was not significantly affected. Attenuation of the BaMV symptoms also was observed in Chenopodium quinoa and Nicotiana benthamiana plants co-inoculated with BSL6 satBaMV. In protoplasts of barley and N. benthamiana, BSF4 and BSL6 variants replicated at a similar level whereas in the inoculated leaves of C. quinoa and N. benthamiana BSL6 accumulated to a much lower level than BSF4, and was encapsidated. BSL6 satBaMV was rarely detected in the systemic leaves of N. benthami-ana co-inoculated with BaMV RNA, indicating that BSL6 satBaMV may be a movement-inefficient variant. It is suggested that this variant may provide a potential source for both resistance against BaMV and insights into the interactions of satellite RNA, helper virus, and symptom expression.

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Section: Discussionmentioning

confidence: 80%

Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants

Hsu¹,

Lee²,

Liu³

et al. 1998

MPMI

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“…Pseudorecombinant viruses made between CMV and TA V, by exchange of RNA 3, were used to map the differential satellite support functions to RNAs I and/or 2, and not to the coat protein gene on RNA 3 (MORIONES et al 1994). Thus, specific interactions with either RNAs land 2 or their encoded genes (I a, 2a, 2b) are responsible for systemic movement of satellite RNAs.…”

Section: Helper Virus Effects On Replicationmentioning

confidence: 99%

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

García‐Arenal

Palukaitis²

1999

Current Topics in Microbiology and Immunology

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“…In contrast, for CMV, there is evidence that the promoter of (−)-strand RNA synthesis can be recognized by certain heterologous viral replicases. For instance, pseudo-recombinants and recombinants created with CMV and Tomato aspermy virus (TAV) can replicate normally both in protoplasts and in entire plants (Perry & Francki, 1992 ; Moriones et al ., 1994 ; Salánki et al ., 1997 ). In addition, a BMV recombinant in which the 3′ terminus of RNA3 was replaced by that of CMV could be replicated by BMV replicase (Rao & Grantham, 1994 ), suggesting that the RdRp of the more distantly related BMV can also recognize the CMV 3′-terminal promoter.…”

Section: Full Textmentioning

confidence: 99%

“…In contrast, for CMV, there is evidence that the promoter of (k)-strand RNA synthesis can be recognized by certain heterologous viral replicases. For instance, pseudorecombinants and recombinants created with CMV and Tomato aspermy virus (TAV) can replicate normally both in protoplasts and in entire plants (Perry & Francki, 1992 ;Moriones et al, 1994 ;Sala! nki et al, 1997).…”

mentioning

confidence: 99%

Synthesis of (−)-strand RNA from the 3′ untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses

Teycheney¹,

Aaziz

Dinant³

et al. 2000

Journal of General Virology

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Potyviruses and cucumoviruses are agronomically important groups of (j)-stranded plant RNA viruses, but they are strikingly different both structurally and in their modes of expression and replication. The potyviruses (Potyviridae) are members of the Picorna-associated supergroup, and have a monopartite, polyadenylated RNA genome, which is covalently linked to a small peptide (VPg) at its 5h end. The viral RNA is encapsidated in flexuous rod-shaped particles, and encodes a single polyprotein which is co-and post-translationally processed by three virus-encoded proteases (Dougherty & Semler, 1993). Cucumoviruses (Bromoviridae) are members of the Alpha-associated supergroup, and their genome is composed of one monocistronic and two bicistronic RNAs, which are encapsidated in icosahedral particles. The 5h

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Mapping Helper Virus Functions for Cucumber Mosaic Virus Satellite RNA with Pseudorecombinants Derived from Cucumber Mosaic and Tomato Aspermy Viruses

Cited by 8 publications

References 0 publications

Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants

Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

Synthesis of (−)-strand RNA from the 3′ untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses

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