Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha

Zhang, Yibin; Wang, Zhen; Quan, Wei; Zhang, Xiang; Jing, Feng; Ren, Jingyao; Jiang, Xu; Zhang, Zhongjun

doi:10.1007/s00122-021-03776-4

Cited by 4 publications

(5 citation statements)

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“…During this period, Pt population were considerably diverse in terms of virulence variants. For example, seven Pt races were identified from only 30 samples collected from Gansu plots in 2014 ( Du et al, 2015 ), and eleven Pt races were identified from 40 samples from Shandong plots in 2018 ( Zhang et al, 2021 ), which is much fewer than the actual number of naturally occurring races due to the limited number of samples analyzed. Comprehensive surveys of Pt races have been performed over the wheat-growing areas of China by other researchers.…”

Section: Discussionmentioning

confidence: 99%

“…To date, 80 designated leaf rust resistance (Lr) genes and numerous quantitative trait loci (QTL) have been described ( Li et al, 2014 ; McIntosh et al, 2017 ; Pinto da Silva et al, 2018 ; Kumar et al, 2021 ; Zhang et al, 2021 ). Leaf rust resistance is commonly categorized into two types, namely, all stage resistance (ASR) and adult plant resistance (APR).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

et al. 2022

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Leaf rust caused by Puccinia triticina Eriks. (Pt) is a common disease of wheat worldwide. The Chinese wheat landrace Bai Qimai (BQM) has shown high resistance to leaf rust for a prolonged period of time; the infected leaves of BQM displayed high infection types (ITs), but they showed low disease severities at the adult plant stage. To find quantitative trait loci (QTL) for resistance to leaf rust, 186 recombinant inbred lines from the cross Nugaines × BQM were phenotyped for leaf rust response in multiple field environments under natural Pt infections and genotyped using the 90K wheat single nucleotide polymorphism (SNP) chip and simple sequence repeat (SSR) markers. A total of 2,397 polymorphic markers were used for QTL mapping, and a novel major QTL (QLr.cau-6DL) was detected on chromosome 6DL from BQM. The effectiveness of QLr.cau-6DL was validated using the three additional wheat populations (RL6058 × BQM, Aikang58 × BQM, and Jimai22 × BQM). QLr.cau-6DL could significantly reduce leaf rust severities across all tested environments and different genetic backgrounds, and its resistance was more effective than that of Lr34. Moreover, QLr.cau-6DL acted synergistically with Lr34 to confer strong resistance to leaf rust. We believe that QLr.cau-6DL should have high potential value in the breeding of wheat cultivars with leaf rust resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

et al. 2022

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“…In southern Gansu, trial plots were located in Wushan county (34°42′15′′N, 104°40′08″E, and elevation 1650 m). The trial was carried out in the same ways as in Zhang et al (2021). Briefly, each plot was constituted with a single 1-m-long row spaced 25 cm apart.…”

Section: Selection Of 112 Rils From Sw × Qsm and Disease Assessmentmentioning

confidence: 99%

“…To minimize a possible cold damage to the wheat plants that harbor Pst, the test plots were protected using plastic films in winter months. On drought days in autumn, spring and the early-summer, the test wheat plants were frequently atomized with water after sunset to facilitate Pst infection as we previously detailed (Wang et al, 2019;Zhang et al, 2021). Pst samples collected from infected MX plants were differentiated for their pathotypes applying the methods used in the USA (Wan and Chen, 2014), but choosing 17 Yr genes as differentials that have high differential ability in China (Supplementary Table 2).…”

Section: Selection Of 112 Rils From Sw × Qsm and Disease Assessmentmentioning

confidence: 99%

“…Initial urediniospores of the races were kindly provided by Chen et al (2009) and Dr. Ruiming Lin (Institute of Plant Protection, Chinese Academy of Agricultural Science). Propagation of urediniospores and inoculation were performed as detailed in Wang et al (2019) and Zhang et al (2021). Briefly, the urediniospores were increased on MX plants grown in a greenhouse, and fresh urediniospores were harvested just before they were needed for inoculating the field trial plants.…”

Section: Selection Of 112 Rils From Sw × Qsm and Disease Assessmentmentioning

confidence: 99%

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Mapping and validation of a novel major QTL for resistance to stripe rust in four wheat populations derived from landrace Qishanmai

et al. 2023

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Wheat yield has been constrained by stripe rust disease globally. A wheat landrace (Qishanmai, QSM) consistently showed lower stripe rust severities in multiple year studies than susceptible check varieties including Suwon11 (SW) at the adult plant stage. To detect QTL for reducing the severity in QSM, 1218 recombinant inbred lines (RILs) were developed from SW × QSM. QTL detection was conducted firstly using 112 RILs selected for similarity in pheno-morphological characters. The 112 RILs were assessed for stripe rust severity at the 2nd leaf, 6th leaf and flag leaf stages under field and greenhouse conditions, and genotyping was done primarily with a single nucleotide polymorphism (SNP) array. On the basis of these phenotypic and genotypic data, a major QTL (QYr.cau-1DL) was detected on chromosome 1D at the 6th leaf and flag leaf stages. Further mapping was conducted by genotyping 1218 RILs using new simple sequence repeat (SSR) markers, which were developed by referring to the sequences of the wheat line Chinese Spring (IWGSC RefSeq v1.0). QYr.cau-1DL was mapped within a 0.5 cM (5.2 Mb) interval delimited by the SSR markers 1D-320.58 and 1D-325.79. These markers were applied to select for QYr.cau-1DL by screening F2 or BC4F2 plants of the wheat crosses RL6058 × QSM, Lantian10 × QSM and Yannong21 × QSM. F2:3 or BC4F2:3 families derived from the selected plants were assessed for stripe rust resistance in the fields of two locations and in a greenhouse. Wheat plants carrying the resistant marker haplotype in homozygous state for QYr.cau-1DL showed lower stripe rust severities (by 44% to 48%) than plants lacking this QTL. The trial of RL6058 (a carrier of Yr18) × QSM also indicated that QYr.cau-1DL had larger effect than Yr18 on reducing severity; they acted synergistically, yielding an elevated level of stripe rust resistance.

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Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670

Baranwal,

Bansal,

Bariana

2024

Trop. plant pathol.

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Transfer of leaf resistance genes into elite lines can assist in developing future wheat cultivars and mitigate economic losses caused by the leaf rust pathogen, Puccinia triticina Erikss. (Pt). Some previously reported leaf rust resistance QTL have been challenged by aggressive Pt pathotypes. This experiment aims to detect genetically diverse resistance QTL using a Tunisian landrace, Aus26670, which confers a high level of adult plant leaf rust resistance against Australian Pt pathotypes. One hundred nineteen F7 recombinant inbred lines (RILs) were generated after crossing Aus26670 and a susceptible line Avocet ‘S’ (AvS). The Aus26670/AvS RIL population was evaluated against mixture of five Pt pathotypes under field conditions for two years. The same RIL population was also screened using three Pt pathotypes individually in the greenhouse under controlled conditions. Genetic analysis of the seedling leaf rust response against Pt104-2,3,6, (7) confirmed the presence of an all-stage resistance (ASR) gene, Lr13. The RIL population was genotyped using a targeted genotyping-by-sequencing (tGBS) assay, and Lr13 was mapped in the 153.9–182.1 Mb region in chromosome 2BS. QTL analysis suggested the involvement of three genomic regions/adult plant resistance genes (APR) namely QLr.sun-1BL/Lr46, QLr.sun-5DL and QLr.sun-7DS, in controlling leaf rust resistance. Data of seedling assays, known marker survey, and comparison of genomic regions detected in this study with previously reported APR indicated the uniqueness of QLr.sun-5DL (559.7 Mb) and QLr.sun-7DS (11 Mb). Both QTL represent new additions to the APR toolbox.

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Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha

Cited by 4 publications

References 45 publications

Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

Mapping and validation of a novel major QTL for resistance to stripe rust in four wheat populations derived from landrace Qishanmai

Detection of new adult plant leaf rust resistance loci in a Tunisian wheat landrace Aus26670

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