Mapping of antigenic determinants of hepatitis C virus proteins using phage display

Rechkina, Elena; Denisova, Galina; Масалова, О. В.; Lideman, L. F.; Denisov, D. A.; Леснова, Е. И.; Ataullakhanov, Ravshan; Гурьянова, С В; Кущ, А А

doi:10.1134/s002689330602018x

Cited by 22 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To study the efficiency of HCV gene expression, indirect immunofluorescence staining was performed using 20 original monoclonal antibodies (mABs) against NS3, NS4A, NS4B, and NS5A [20] and commercial mABs against structural and nonstructural HCV proteins, including E1 (ab21306), E2 (ab20852) (Abcam, United Kingdom), and NS5B (SC-58146) (Santa Cruz Biotechnology, United States). FITC-conjugated goat anti-mouse immunoglobulin (ab97039, Abcam) was used as a secondary antibody.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hepatitis C virus proteins on the production of proinflammatory and profibrotic cytokines in Huh7.5 human hepatoma cells

et al. 2016

Self Cite

View full text Add to dashboard Cite

Hepatitis C virus (HCV) is a widespread dangerous human pathogen. Up to 80% of HCV-infected individuals develop chronic infection, which is often accompanied by liver inflammation and fibrosis and, at terminal stages, liver cirrhosis and cancer. Treatment of patients with end-stage liver disease is often ineffective, and even patients with suppressed HCV replication have higher risk of death as compared with noninfected subjects. Therefore, investigating the mechanisms that underlie HCV pathogenesis and developing treatments for virus-associated liver dysfunction remain an important goal. The effect of individual HCV proteins on the production of proinflammatory and profibrotic cytokines in hepatocellular carcinoma Huh7.5 cells was analyzed in a systematic manner. Cells were transfected with plasmids encoding HCV proteins. Cytokine production and secretion was accessed by immunocytochemistry and ELISA of the culture medium, and transcription of the cytokine genes was assessed using reverse transcription and PCR. HCV proteins proved to differ in effect on cytokine production. Downregulation of interleukin 6 (IL-6) production was observed in cells expressing the HCV core, NS3, and NS5A proteins. Production of transforming growth factor β1 (TGF-β1) was lower in cells expressing the core proteins, NS3, or E1/E2 glycoproteins. A pronounced increase in production and secretion of tumor necrosis factor α (TNF-α) was observed in response to expression of the HCV E1/E2 glycoproteins. A higher biosynthesis, but a lower level in the cell culture medium, was detected for interleukin 1β (IL-1β) in cells harboring NS4 and IL-6 in cells expressing NS5В. The finding was possibly explained by protein-specific retention and consequent accumulation of the respective cytokines in the cell.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of Hepatitis C virus proteins on the production of proinflammatory and profibrotic cytokines in Huh7.5 human hepatoma cells

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Expression of HCV proteins in the transfected MSCs was determined by the methods of indirect immunofluorescence and immunoperoxidase staining, using monoclonal antibodies (mAbs) against HCV proteins [33] as primary antibodies and secondary antibodies against mouse immunoglobulins (Ig) conjugated with fluorescein isothiocyanate (FITC) or horseradish peroxidase (HRP) (Dako, Denmark), as previously described [34,35]. Cell nuclei were stained with 4 -6-diamidino-2-phenylindole (DAPI) (immunofluorescence analysis) or with hematoxylin (immunoperoxidase method).…”

Section: Immunocytochemical and Immunoblot Detection Of Viral Proteinsmentioning

confidence: 99%

Genetically Modified Mouse Mesenchymal Stem Cells Expressing Non-Structural Proteins of Hepatitis C Virus Induce Effective Immune Response

et al. 2020

View full text Add to dashboard Cite

Hepatitis C virus (HCV) is one of the major causes of chronic liver disease and leads to cirrhosis and hepatocarcinoma. Despite extensive research, there is still no vaccine against HCV. In order to induce an immune response in DBA/2J mice against HCV, we obtained modified mouse mesenchymal stem cells (mMSCs) simultaneously expressing five nonstructural HCV proteins (NS3-NS5B). The innate immune response to mMSCs was higher than to DNA immunization, with plasmid encoding the same proteins, and to naïve unmodified MSCs. mMSCs triggered strong phagocytic activity, enhanced lymphocyte proliferation, and production of type I and II interferons. The adaptive immune response to mMSCs was also more pronounced than in the case of DNA immunization, as exemplified by a fourfold stronger stimulation of lymphocyte proliferation in response to HCV, a 2.6-fold higher rate of biosynthesis, and a 30-fold higher rate of secretion of IFN-γ, as well as by a 40-fold stronger production of IgG2a antibodies to viral proteins. The immunostimulatory effect of mMSCs was associated with pronounced IL-6 secretion and reduction in the population of myeloid derived suppressor cells (MDSCs). Thus, this is the first example that suggests the feasibility of using mMSCs for the development of an effective anti-HCV vaccine.

show abstract

“…It should be noted that in the analysis, analo-gous as well as homologous a.r. were conside red (they were grouped according to their physical and chemical properties [15,[18][19][20]), namely:…”

Section: Resultsmentioning

confidence: 99%

“…epitope mapping. The procedure was based on the algorithm described by Rechkina et al [15]. The following modification of the ELISA method was used to evaluate the specificity of binding of phages with McAbs to MOMP.…”

Section: Methodsmentioning

confidence: 99%

New monoclonal antibodies to the Chlamydia trachomatis main outer membrane protein and their immunobiological properties

Galkin¹,

Besarab²,

Gorshunov³

et al. 2019

Ukr.Biochem.J

View full text Add to dashboard Cite

Mapping of antigenic determinants of hepatitis C virus proteins using phage display

Cited by 22 publications

References 32 publications

Effect of Hepatitis C virus proteins on the production of proinflammatory and profibrotic cytokines in Huh7.5 human hepatoma cells

Effect of Hepatitis C virus proteins on the production of proinflammatory and profibrotic cytokines in Huh7.5 human hepatoma cells

Genetically Modified Mouse Mesenchymal Stem Cells Expressing Non-Structural Proteins of Hepatitis C Virus Induce Effective Immune Response

New monoclonal antibodies to the Chlamydia trachomatis main outer membrane protein and their immunobiological properties

Contact Info

Product

Resources

About