Mapping of calpastatin and three microsatellites to porcine chromosome 2q2.1-q2.4

Ernst, Catherine W.; Robic, Annie; Yerle, Martine M.; Wang, L; Rothschild, Max F.

doi:10.1046/j.1365-2052.1998.00319.x

Cited by 16 publications

(14 citation statements)

References 18 publications

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“…In MC pigs, there are three possible genotypes at both loci, which are similar to other breeds, such as Yorkshire and Large White (Ernst et al, 1998),…”

Section: Discussionmentioning

confidence: 93%

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Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

Ngu

Thiet²,

Kien³

et al. 2012

Afr. J. Biotechnol.

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Calpastain (CAST) activity plays a major role in muscle growth and proteolytic changes post-mortem and the CAST gene has been considered as a candidate gene for carcass and pork quality characteristics. The aim of this study was to analyze the association of two polymorphisms namely CAST_HinfI (allele A and B) and CAST_MspI (allele C and D) with carcass and meat quality traits in Mongcai, a Vietnamese indigenous pig breed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype the animals at these loci. Results indicate that the CAST_HinfI single nucleotide polymorphism (SNP) had a low frequency of allele A as compared to allele B, while the C and D allele distribution was almost the same for the CAST_MspI SNP. In the association analysis, significant effects on dressing percentage of carcass were detected. The CAST_HinfI locus was associated with the pH 24 , while the CAST_MspI position was in association with pH 45 min , drip loss 48 and redness color. Additional analysis showed a variation in muscle fiber type composition with higher proportion of IIx fiber in pigs with AB genotype (P < 0.05). Three constructed haplotypes namely AB/CD, AB/DD and BB/CC also had significant effects on carcass, type IIa and IIb fiber percentages.

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“…In MC pigs, there are three possible genotypes at both loci, which are similar to other breeds, such as Yorkshire and Large White (Ernst et al, 1998),…”

Section: Discussionmentioning

confidence: 93%

“…The primer sequence and PCR conditions to amplify the product of the CAST gene (GenBank Accession No. EU137105.1) were applied following the method detailed by Ernst et al (1998). For genotyping, genetic variants were identified by the PCR-RFLP method using two restriction Ngu et al 13783 enzymes namely HinfI and MspI (MBI, Fermentas).…”

Section: Genotypingmentioning

confidence: 99%

Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

Ngu

Thiet²,

Kien³

et al. 2012

Afr. J. Biotechnol.

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show abstract

“…Total DNA was isolated from 50 mg of pig tissue using a commercially available High Pure PCR Template Kit (Roche Diagnostics GmbH, Mannheim, Germany), according to the protocol provided by manufacturer. The genotypes at different CAST loci were identified with HinfI, MspI and RsaI restriction enzymes according to the method described by Ernst et al (1998). PCR was performed in a reaction mixture of 20 mL containing 50 ng genomic DNA, standard PCR buffer, 1.5 mM MgCl2, 200 mM of each dNTP, 5 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, Vilnius, Lithuania).…”

Section: Dna Analysismentioning

confidence: 99%

“…Unlike calpains, which are coded by at least 14 genes (Kemp et al 2010), calpastatin is coded by only one gene -CAST, mapped on SSC2 in Region 2q2.1-q2.4 (Ernst et al 1998). Ciobanu et al (2004) sequenced complete CAST gene and identified two single nucleotide polymorphisms (SNPs) associated with meat tenderness, namely Arg249 Lys and Ser638Arg.…”

Section: Introductionmentioning

confidence: 99%

Differences in carcass traits, meat quality and chemical composition between the pigs of different CAST genotype

Kušec

Vuković

et al. 2016

Anim. Prod. Sci.

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The study was carried out on 89 Pig Improvement Co. (PIC) pig carcasses, with the aim to investigate the differences between three CAST loci in carcass and meat-quality traits, as well as chemical composition of longissimus dorsi (LD) muscle. The differences among genotypes at CAST/HinfI locus were significant in all carcass traits measured, where AB genotype exhibited preferable values in carcass lengths, ham length, muscle thickness, loin eye area, fat thickness and fat area. Among meat-quality traits analysed, genotypes at CAST/HinfI locus differed in pH45 in SM muscle, both pH24 in semimembranosus (SM) and LD muscles, as well as luminosity; genotypes at CAST/MspI differed in pH24 and EC24 measured at LD muscle and in red colour intensity, level of yellowness and hue angle; while genotypes at CAST/RsaI differed in pH45 and EC45 in SM muscle, pH24 in LD muscle, paleness and redness, as well as in shear force and calpain activity. EF genotype at this locus exhibited the highest pH values and the lowest CIE L*, with more pronounced red colour, but also highest shear force and lowest calpain activity values. Furthermore, significant differences in chemical composition of LD muscle were found only among genotypes at CAST/RsaI loci, where FF genotype had the lowest intramuscular fat and the highest relative share of protein.

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“…Therefore, this gene affects the proteolytic system of meat during the post mort period (KRISTENSEN et al, 2002;ROPKA-MOLIK et al, 2014). The calpastatin gene (CAST) is located on chromosome 2 (2q2.1-2q2.4) (ERNST et al, 1998;DAVOLI et al, 2017). CIOBANU et al (2004) reported an association analysis of a missense mutation (Ser638Arg) in the CAST gene where quantitative trait loci (QTL) for meat quality such as shear force and tenderness,were described.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Ser638Arg variation in the cast gene and causal SNP g.1843C>T in the RYR1 gene on carcass traits in crosbreed pigs

et al. 2019

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The objectives of this investigation was to demonstrate that genotypes of calpastatin (CAST) Ser368Arg and ryadonide receptor 1 (RYR1) g.1843C>T may affect the carcass traits of pigs. Association analysis of the aforementioned SNPs was performed on 518 pigs included eight commercial crossbreeds and one pure pig breed. All pigs were slaughtered at average body weight of 113kg. Genotypes at the SNPs were determined by PCR-RFLP. There were found only two genotypes of RYR1 gene: CC (428 pigs) and CT (90 pigs) in this study. The effect of allele C compared to allele T on the higher fat content of pig carcass was confirmed. The allelic frequencies of allele 638Ser (C) and allele 638Arg (A) were 0.26 and 0.74, respectively. The significant association (P<0.05) was occurred between allele A and higher fat content, and between C allele and higher lean meat content. Our results showed no significant effect of the observed polymorphismSer638Arg on the intramuscular fat content or other indicators of carcass value.

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Mapping of calpastatin and three microsatellites to porcine chromosome 2q2.1-q2.4

Cited by 16 publications

References 18 publications

Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

Differences in carcass traits, meat quality and chemical composition between the pigs of different CAST genotype

Effect of the Ser638Arg variation in the cast gene and causal SNP g.1843C>T in the RYR1 gene on carcass traits in crosbreed pigs

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