Mapping of centromeric regions on the molecular linkage map of rice (Oryza sativa L.) using centromere-associated sequences

Wang, Shanming; Wang, J.; Jiang, Jiming; Zhang, Q.

doi:10.1007/s004380050044

Cited by 20 publications

(13 citation statements)

References 35 publications

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“…Three-way crosses have been applied successfully in rice to map and identify QTLs for wide-compatibility (Wang et al 1998), to map centromeric regions (Wang et al 2000), and to map R genes (Wang and Xiao 2002).…”

Section: Discussionmentioning

confidence: 99%

Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

Dahleen¹,

Agrama

Horsley

et al. 2003

Theor Appl Genet

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Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.

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Section: Discussionmentioning

confidence: 99%

Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

Dahleen¹,

Agrama

Horsley

et al. 2003

Theor Appl Genet

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“…The triangles in the bacterial blight resistance and blast resistance QTLs, detected using the same population as was used to construct the framework map, indicate the logarithm-of-odds peaks of the resistance effects. The filled circles represent the centromeric regions identified previously (Wang et al 2000a) co-localize with resistance QTLs identified in barley, bean, rice and potato (Faris et al 1999;Geffroy et al 2000;Trognitz et al, 2002;Xiong et al 2002;Ramalingam et al 2003). Thus, the candidate-gene approach may provide a means for pinpointing the genes that underlie resistance QTLs.…”

Section: Analyses Of the Sequences And Expression Patterns Of Defensementioning

confidence: 97%

Genome-wide analysis of defense-responsive genes in bacterial blight resistance of rice mediated by the recessive R gene xa13

et al. 2004

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Defense responses triggered by dominant and recessive disease resistance (R) genes are presumed to be regulated by different molecular mechanisms. In order to characterize the genes activated in defense responses against bacterial blight mediated by the recessive R gene xa13, two pathogen-induced subtraction cDNA libraries were constructed using the resistant rice line IRBB13--which carries xa13--and its susceptible, near-isogenic, parental line IR24. Clustering analysis of expressed sequence tags (ESTs) identified 702 unique expressed sequences as being involved in the defense responses triggered by xa13; 16% of these are new rice ESTs. These sequences define 702 genes, putatively encoding a wide range of products, including defense-responsive genes commonly involved in different host-pathogen interactions, genes that have not previously been reported to be associated with pathogen-induced defense responses, and genes (38%) with no homology to previously described functional genes. In addition, R-like genes putatively encoding nucleotide-binding site/leucine rich repeat (NBS-LRR) and LRR receptor kinase proteins were observed to be induced in the disease resistance activated by xa13. A total of 568 defense-responsive ESTs were mapped to 588 loci on the rice molecular linkage map through bioinformatic analysis. About 48% of the mapped ESTs co-localized with quantitative trait loci (QTLs) for resistance to various rice diseases, including bacterial blight, rice blast, sheath blight and yellow mottle virus. Furthermore, some defense-responsive sequences were conserved at similar locations on different chromosomes. These results reveal the complexity of xa13-mediated resistance. The information obtained in this study provides a large source of candidate genes for understanding the molecular bases of defense responses activated by recessive R genes and of quantitative disease resistance.

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“…It ensures proper cell division and stable transmission of the genetic material (Wang et al 2000). Elucidating the composition and structure of centromeres can be of use to understand its functional roles, including chromosome segregation, karyotypic stability, and artificial chromosome-based cloning (Wu et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Genetic Positioning of Centromeres Using Half-Tetrad Analysis in a 4x–2xCross Population of Potato

Park¹,

Kim²,

Hutten

et al. 2007

Genetics

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From biological and genetic standpoints, centromeres play an important role in the delivery of the chromosome complement to the daughter cells at cell division. The positions of the centromeres of potato were determined by half-tetrad analysis in a 4x-2x population where the male parent produced 2n pollen by first-division restitution (FDR). The genetic linkage groups and locations of 95 male parent-derived amplified fragment length polymorphism markers could be determined by comparing their position on a 2x-2x highly saturated linkage map of potato. Ten centromere positions were identified by 100% heterozygosity transmitted from the 2n heterozygous gametes of the paternal parent into the tetraploid offspring. The position of these centromeric marker loci was in accordance with those predicted by the saturated 2x-2x map using the level of marker clustering as a criterion. Two remaining centromere positions could be determined by extrapolation. The frequent observation of transmission of 100% heterozygosity proves that the meiotic restitution mechanism is exclusively based on FDR. Additional investigations on the position of recombination events of three chromosomes with sufficient numbers of markers showed that only one crossover occurred per chromosome arm, proving strong interference of recombination between centromere and telomere.

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Mapping of centromeric regions on the molecular linkage map of rice (Oryza sativa L.) using centromere-associated sequences

Cited by 20 publications

References 35 publications

Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

Genome-wide analysis of defense-responsive genes in bacterial blight resistance of rice mediated by the recessive R gene xa13

Genetic Positioning of Centromeres Using Half-Tetrad Analysis in a 4x–2xCross Population of Potato

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