Mapping of Cleavage Sites for Restriction Endonucleases in λdv Plasmids

Abstract

Restriction nucleases from Escherichia coli carrying the resistance transfer factor RI, from Hemophilus injluenzae, Hemophilus purainfluenzae, and Bacillus subtilis were used for the generation of specific DNA fragments from idv plasmids. Cleavage maps were established for various plasmids containing different but overlapping parts of bacteriophage A DNA by analysis of digestion patterns obtained in polyacrylamide gel electrophoresis. The correlation between the plasmid cleavage maps and the genetic map of 1, was based upon the location of the EcoRI cleavage site at 81.0% of 3. fractional length and the position of promoters pL and pK on the plasinid DNA fragments.

A Long-Range and Two Short-Range Periodicities Are Superimposed in the 1.706-g/cm3 Satellite DNA from Calf Thymus

“…The sizes of the PM2 and Adv fragments were determined from between four and seven gels or, for the two smallest fragments, from two gels. Mean values and standard errors of the mean for Bsp fragments of PM2 are given in base These values are slightly different from published ones [23]. Fragments Q and R probably correspond to fragments Q2 and Q1, respectively, of [23].…”

“…Mean values and standard errors of the mean for Bsp fragments of PM2 are given in base These values are slightly different from published ones [23]. Fragments Q and R probably correspond to fragments Q2 and Q1, respectively, of [23]. The size of Advl determined as the sum of the fragment sizes is 7140 f 40 base pairs.…”

“…The size of Advl determined as the sum of the fragment sizes is 7140 f 40 base pairs. Analogous determinations have led to 2305 & 15 base pairs and 3080 f 20 base pairs for ldvh93 and ldv021 [23], respectively. Partial EcoRI or Hind111 digests of the octamer of Adv021 (stably maintained in recA -strains obtained from G. Hobom) provided a useful set of eight regularly spaced bands in gel electrophoresis up to 24600 f 150 base pairs.…”

“…4x174 wild type, E. coli C, and a sample of 4x174 RFI DNA were kindly donated by P. Weisbeek. PM2 and ldvl DNA were prepared as described [22,23].…”

Closely Spaced Nucleosome Cores in Reconstituted Histone . DNA Complexes and Histone-H1-Depleted Chromatin

“…Esclz~~ricliiu coli strains m53 (idv 1) and 866 (idv 21) which contained the plasmids idv 1 as a monomer and i.dv 21 as ; I dimer were obtained from G. Hobom (Freiburg). Isolation of the closed circular forms of the plasinid DNAs from 10-1 cultures was carried out according to the protocol for 1-1 cultures given by Streeck and Hobom [26] with minor modifications [27].…”