Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites

Inselburg, Joseph; Johns, Virginia

doi:10.1128/jb.121.1.381-389.1975

Cited by 14 publications

(7 citation statements)

References 12 publications

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“…The molecular weights of the HaeII-generated fragments were determined by comparing them with EcoRI-generated fragments of ColEl, ColE2, and ColE3 plasmid DNAs and X phage DNA (10). ColEl is digested into six fragments ( Fig.…”

Section: Isolation Of Deletion Mutants Of Colel It Was Previously Shmentioning

confidence: 99%

Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion

Inselburg¹,

Ware²

1977

J Bacteriol

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Deletions of colicin El (colEl) plasmid deoxyribonucleic acid (DNA) carrying the TnA transposon have been isolated. All except two were generated by nuclease digestion of plasmid DNA from its EcoRI-sensitive site. A plasmid containing about 16% of the ColEl DNA (6.5 x 105 daltons) was generated that also contained the part of the TnA transposon conferring ampicillin resistance. The extents of different deletions were determined by analysis of restriction endonuclease fragments generated by the restriction endonucleases HaeII, BamHI, and HincII. NaCl plus 0.015 M sodium citrate).Enzymatic digestions of DNA. The endonucleases used were EcoRI, HaeII, BamHI, and HincII, which were obtained from New England Biolabs. Most HaeII used was a generous gift of H. Ohmori and J. Tomizawa. DNA digestions were conducted overnight at 37°C. EcoRI digestions of DNA were 321 on July 31, 2020 by guest

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Section: Isolation Of Deletion Mutants Of Colel It Was Previously Shmentioning

confidence: 99%

Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion

Inselburg¹,

Ware²

1977

J Bacteriol

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“…Homology is also evident in their corresponding plasmids ColE2-P9 and ColE3- CA38. Heteroduplex experiments (7,8) and a comparison of restriction maps (9,10) revealed that the major difference between these two plasmids is to be found around the limited region encoding the T2A and immunity protein of each colicin.…”

Section: Introductionmentioning

confidence: 99%

Structure and expression of the ColE2-P9 immunity gene

1985

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The primary structure and expression of the ColE2-P9 immunity gene (imm) were investigated. The imm gene is located behind the colicin gene (col) in the same orientation with an intergenic space of two base pairs. Although the imm gene was transcribed primarily in response to the SOS function of the host cell as well as the col gene, the immunity phenotype also appeared to be expressed by only a slight level of leaky transcription without an evident promoter. On comparing the ColE2-P9 sequence with those of relevant plasmids, a highly homologous sequence with the immE2 gene was found downstream of the immE3 gene of ColE3-CA38, and thus, an evolutional relationships could be deduced among some E-group Col plasmids.

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“…Locations of the colicin and immunity genes. ColE3-CA38 and ColE2-P9 are 80 to 90% homologous, and their restriction maps are very similar (5,10). Because the positions of the colicin and immunity genes of ColE3-CA38 are known (R. Watson and L. P. Visentin, submitted for publication), the positions of these genes Restriction maps and regions of nonhomology of ColE2-P9, ColE2-CA42, and ColE2-pBR322 hybrid plasmids.…”

mentioning

confidence: 99%