Mapping of estrogen receptor-producing cells in the rat brain by in situ hybridization

Pelletier, Georges; Liao, Ning; Follea, N.; Govindan, Manjapra V.

doi:10.1016/0304-3940(88)90264-9

Cited by 85 publications

(34 citation statements)

References 9 publications

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“…8B) 3) Discussion The in situ hybridization findings reported here demonstrated selective distribution of ERmRNA in the central hypothalamus, amygdala and uterus. The sites of ERmRNA-containing neurons were largely in agreement with those of ER in the previous autoradiographical (40) and immunohistochemical (48) studies, and with the in situ hybridization ones (42,43,50). Discrepancy exists, however, on the existence of ERmRNA in the amygdala between ours and Pelletier's (42).…”

Section: ) Ermrna Analysis In Uterine Endometrial Cancerssupporting

confidence: 58%

“…Our preliminary results have revealed the existence of ERmRNA even in poorly differentiated cancers in which ERs are absent, implying different regulation of the ER level in the cancer lacking in hormone dependency. Several in situ hybridization studies have reported the distribution of ERmRNA containing neurons in the rat brain (42,43,50), which is roughly similar as that of ER (40,48).…”

Section: ) Ermrna Analysis In Uterine Endometrial Cancersmentioning

confidence: 84%

See 1 more Smart Citation

Gene expression of steroid hormone receptors in brain and peripheral tissues relative to reproduction.

Kato¹,

Hirata²,

Hagihara³

et al. 1992

Acta Histochem. Cytochem

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Section: ) Ermrna Analysis In Uterine Endometrial Cancerssupporting

confidence: 58%

Section: ) Ermrna Analysis In Uterine Endometrial Cancersmentioning

confidence: 84%

Gene expression of steroid hormone receptors in brain and peripheral tissues relative to reproduction.

Kato¹,

Hirata²,

Hagihara³

et al. 1992

Acta Histochem. Cytochem

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“…Estrogen receptors are widely distributed in the brain, including the limbic system and the hypothalamus (48). Our findings implicate the CRH gene and, therefore, the HPA axis, as a potentially important target of ovarian steroids and a potential mediator of gender related differences in the stress response and HPA axis activity.…”

Section: Resultsmentioning

confidence: 81%

Evidence of direct estrogenic regulation of human corticotropin-releasing hormone gene expression. Potential implications for the sexual dimophism of the stress response and immune/inflammatory reaction.

Vamvakopoulos

Chrousos²

1993

J. Clin. Invest.

408

206

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Corticotropin-releasing hormone (CRH) plays major roles in coordination of the stress response and regulation of the immune/inflammatory reaction, two important functions associated with sexual dimorphism. Two overlapping segments of the 5' flanking region of the human (h) CRH gene, the proximal 0.9 kb (containing two perfect half-palindromic estrogenresponsive elements IEREsI) and the 2.4 kb (including the former and containing two additional perfect half-palindromic EREs), were examined for their ability to confer estrogen-mediated transcriptional enhancement to a homologous or heterologous promoter. The level of estrogen-induced transactivation by the 0.9-and 2.4-kb segments was determined by chloramphenicol acetyltransferase analysis in CV-1 cells cotransfected with estrogen receptor (ER) cDNA expression plasmids, and found to be respectively -10% and 20% of that of the strongly estrogen-responsive Xenopus vitellogenin A2 enhancer. Gel retardation and immunoprecipitation demonstrated specific association between the perfect half-palindromic EREs of hCRH gene and the DNA binding domain of hER in vitro. These findings may constitute the basis of sexual dimorphism in the expression of the CRH gene in the central nervous system and periphery, and might shed light in existing gender differences in stress response and immune regulation. (J. Clin. Invest.

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“…What is the mechanism by which a previously unresponsive neuronal type becomes responsive? Although estrogens can act to alter gene transcription (McEwen et al, 1978), it is unlikely that this is the case in the present study because in the hippocampus, both ␣-and ␤-estrogen receptors have been identified on interneurons and glial cells and not on dentate granule cells (Rainbow et al, 1982;L oy et al, 1988;Pelletier et al, 1988;Weiland et al, 1996;Kuiper et al, 1997;Li et al, 1997). Estradiol might act directly on membranes, altering their properties to induce glutamate release, thus increasing intraneuronal calcium, stimulating NMDA receptors, leading to the addition of dendritic spines (Parpura et al, 1994;Woolley and McEwen, 1994;K lintsova et al, 1995).…”

Section: Discussionmentioning

confidence: 94%

Granule Cells in Aging Rats Are Sexually Dimorphic in Their Response to Estradiol

1999

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Normal aging comprises cognitive decline, including deterioration of memory. It has been suggested that this decline in memory is sexually dimorphic because of the cessation in gonadal steroid secretion that occurs during reproductive aging in female, but not male, mammals. We wondered whether neurons in brain regions associated with learning and memory underwent morphological changes that were dimorphic as well and whether cessation of the secretion of gonadal steroids influenced these morphological changes. To explore these questions, we deprived and restored estrogens to young and old gonadectomized females and males and studied the morphology of dentate granule cells by intracellular dye filling in a lightly fixed slice preparation. We found the following: (1) Aged female dentate granule cells deprived of gonadal steroids longterm have a paucity of dendritic spines compared with young females deprived short-term; however, aged male dentate granule cells deprived of gonadal steroids long-term have no decrease in dendritic spines compared with young males deprived short-term. (2) Aged female dentate granule cells with long-term estrogen replacement at either high or low levels still had a decline in spine density. (3) Aged female dentate granule cells with short-term estradiol replacement had spine density increased to levels normally observed in young adults, whereas aged males with short-term estradiol replacement had decreased spine density. These data suggest that the response of rat dentate granule cells to aging and estradiol is sexually dimorphic and that, in females, the responsiveness of granule cells depends on the temporal pattern of estradiol replacement.

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Mapping of estrogen receptor-producing cells in the rat brain by in situ hybridization

Cited by 85 publications

References 9 publications

Gene expression of steroid hormone receptors in brain and peripheral tissues relative to reproduction.

Gene expression of steroid hormone receptors in brain and peripheral tissues relative to reproduction.

Evidence of direct estrogenic regulation of human corticotropin-releasing hormone gene expression. Potential implications for the sexual dimophism of the stress response and immune/inflammatory reaction.

Granule Cells in Aging Rats Are Sexually Dimorphic in Their Response to Estradiol

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