Mapping of Hieracium (Asteraceae) chromosomes with genus-specific satDNA elements derived from next-generation sequencing data

Belyayev, Alexander; Paštová, Ladislava; Fehrer, Judith; Josefiová, Jiřina; Chrtek, Jindřich; Mráz, Patrik

doi:10.1007/s00606-017-1483-y

Cited by 21 publications

(34 citation statements)

References 39 publications

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“…Two chromosome pairs possessed terminal blocks of 45S rDNA, and one of these pairs additionally had one 5S rDNA block in the pericentromeric region of the short arm. All chromosomes showed strong hybridization of the centromeric probe HintCl-18 (Belyayev & al., 2018). The karyotype of triploid H. telekianum showed a morphologically and structurally more imbalanced pattern when compared to diploid H. vranceae and consisted of metacentric, submetacentric and one acrocentric chromosome (Fig.…”

Section: ■ Resultsmentioning

confidence: 99%

“…Three chromosomes with 5S rDNA clusters were different from each other, suggesting they belong to three different haplomes. One chromosome was typical for the majority of Hieracium species analysed so far (Ilnicki & al., 2010;Belyayev & al., 2018; and H. vranceae in this paper) when 5S and 45S rDNA blocks were co-located on the short chromosome arm (Fig. 3B1); the second chromosome was acrocentric with the 5S rDNA cluster on the short arm (Fig.…”

Section: ■ Resultsmentioning

confidence: 99%

“…Its genome size fell within the variation of other diploid taxa, more specifically into the range of those belonging to an eastern European phylogenetic clade that shows significantly higher genome size than diploids from a western European clade (Chrtek & al., ). The karyotype of H. vranceae was very similar to those of diploid H. alpinum , H. intybaceum (Belyayev & al., ) and H. transylvanicum (Ilnicki & al., ), but differed from the karyotype of diploid H. prenanthoides , which possessed three instead of two 45S rDNA blocks per haplome (Belyayev & al., ). Hieracium vranceae was shown as being genetically distinct from all other species (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…The squashed root tips were stained by 10% Giemsa solution in phosphate buffer. Fluorescense in situ hybridization (FISH) and probe labelling of 45S rDNA (pTa71), 5S rDNA and centromeric HintCl-18 probes followed procedures described by Belyayev & al. (2018).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

Mráz

Filipaș²,

Bărbos

et al. 2019

TAXON

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Hieracium s.str. (Asteraceae) is one of the largest angiosperm genera notorious for its taxonomic complexity caused by widespread interspecific hybridization. This process is tightly coupled with polyploidization and apomixis – asexual reproduction by seeds, which has ensured the persistence of otherwise sterile interspecific hybrids. As a result, apomictic polyploid taxa dominate the taxonomic diversity of the genus whilst sexual diploid species are extremely rare and mostly confined to southern Europe. As diploid taxa are assumed to be the parents of apomictic lineages, the discovery of any new diploid species is important for understanding evolutionary processes and diversity patterns in the genus. Here, we describe a new diploid species, Hieracium vranceae, a narrow endemic to the Munţii Vrancei (Vrancea Mountains, Eastern Carpathians, Romania). This taxon with a distinctive morphology and a strong affinity to relic rocky habitats was first collected in 2013. This means that, even in Europe, some regions, like the Eastern Carpathians, are botanically underexplored and might still preserve an undiscovered diversity of plants. Phylogenetic analyses with multiple molecular markers (low‐copy nuclear genes gsh1 and sqs, nuclear ribosomal ETS, four cpDNA loci) applied to all diploid Hieracium species support specific rank for H. vranceae. In contrast, molecular data suggest conspecifity of two pairs of species, H. lucidum/H. cophanense from Sicily and H. naegelianum/H. renatae from the Balkans, which are moreover only weakly differentiated morphologically. Molecular (cpDNA) and cytogenetic (GISH, FISH) analyses furthermore revealed that H. vranceae has been involved as a maternal parent in the origin of an allotriploid apomictic species, H. telekianum, to which H. vranceae contributed one haplome. The other putative parents of H. telekianum are the diploid narrow endemic H. pojoritense and probably H. sparsum s.l. Our data thus stress the importance of interspecific hybridization for an evolutionary shift from sexuality to apomixis in Hieracium. Our findings furthermore highlight the significance of that part of the Carpathians as one of the most important evolutionary/refugial centres of Hieracium and suggest dynamic species ranges at a regional scale allowing physical contact of taxa whose distributional ranges are nowadays completely allopatric.

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Section: ■ Resultsmentioning

confidence: 99%

Section: ■ Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

Mráz

Filipaș²,

Bărbos

et al. 2019

TAXON

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“…Among the programs used are Tandem Repeats Finder (Benson, 1999), which uses string matching algorithms, and those utilizing graph-based clustering, such as RepeatExplorer (Novák et al, 2010) and TAREAN (Novák et al, 2017). These programs allow for the mining of existing and public repositories of genomic data to identify tandem repeats for various studies related to phylogenetics, genome evolution, and cytogenetics (Dodsworth et al, 2015;Belyayev et al, 2018;Mlinarec et al, 2019). More recently, long-read sequence data has been used to support FISH probe development in plants, with the aid of RepeatExplorer and TAREAN (Kapustová et al, 2019;Vondrak et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

New Tools for Hop Cytogenomics: Identification of Tandem Repeat Families from Long-Read Sequences ofHumulus lupulus

Ka¹,

Nj²,

et al. 2020

Preprint

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Hop (Humulus lupulus L.) is known for its use as a bittering agent in beer and has a rich history of cultivation, beginning in Europe and now spanning the globe. There are five wild varieties worldwide, which may have been introgressed with cultivated varieties. As a dioecious species, its obligate outcrossing, non-Mendelian inheritance, and genomic structural variability have confounded directed breeding efforts. Consequently, understanding genome evolution in Humulus represents a considerable challenge, requiring additional resources, including integrated genome maps. In order to facilitate cytogenetic investigations into the transmission genetics of hop, we report here the identification and characterization of 17 new and distinct tandem repeat sequence families. A tandem repeat discovery pipeline was developed using kmer filtering and dot plot analysis of PacBio long-read sequences from the hop cultivar Apollo. We produced oligonucleotide FISH probes from conserved regions of HuluTR120 andHulTR225 and demonstrated their utility to stain meiotic chromosomes from wild hop, var.neomexicanus. The HuluTR225 FISH probe hybridized to several loci per nucleus and exhibited irregular, non-Mendelian transmission in male meiocytes of wild hop. Collectively, these tandem repeat sequence families not only represent unique and valuable new cytogenetic reagents but also have the capacity to inform genome assembly efforts and support comparative genomic analyses.

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Evolutionary history and genetic diversity of apomictic allopolyploids in Hieracium s.str.: morphological versus genomic features

Chrtek

Mráz

Belyayev

et al. 2020

American J of Botany

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Mapping of Hieracium (Asteraceae) chromosomes with genus-specific satDNA elements derived from next-generation sequencing data

Cited by 21 publications

References 39 publications

An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

New Tools for Hop Cytogenomics: Identification of Tandem Repeat Families from Long-Read Sequences ofHumulus lupulus

Evolutionary history and genetic diversity of apomictic allopolyploids in Hieracium s.str.: morphological versus genomic features

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