Mapping of major and modifying genes for high oleic acid content in safflower

Hamdan, Yamen; García-Moreno, M. J.; Fernández‐Martínez, José M.; Velasco, Leonardo; Pérez‐Vich, Begoña

doi:10.1007/s11032-012-9714-y

Cited by 32 publications

(22 citation statements)

References 41 publications

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“…In this study we have included another high oleic acid C. tinctorius accession, PI 401479, which accumulates more than 80% oleic acid in its total fatty acids, clearly above the upper-margin of ol mutant (Table 2 ). This very high oleic acid germplasm was identified by Fernández-Martinez et al ( 1993 ) and genetically characterized by Hamdan et al ( 2009 , 2012 ) who demonstrated that the additional oleic acid content (compared to that of PI 599253) was a result of the combined effects of the recessive ol allele and a number of other modifying genes with a positive effect on oleic acid content, as previously suggested by Knowles ( 1972 ). In confirmation of this hypothesis, we found that the FAD2-1 sequences of both PI 599253 and PI 401479 are identical and contain the same genetic mutation of a single nucleotide deletion linked to the high oleic acid trait (data not presented).…”

Section: Discussionsupporting

confidence: 63%

A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.)

Rapson

Okada

et al. 2015

Front. Plant Sci.

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The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while varieties rich in oleic acid have risen to prominence in recent decades. The high oleic acid trait is controlled by a partially recessive allele ol at a single locus OL. The ol allele was found to be a defective microsomal oleate desaturase FAD2-1. Here we present DNA sequence data and Southern blot analysis suggesting that there has been an ancient hybridization and introgression of the FAD2-1 gene into C. tinctorius from its wild relative C. palaestinus. It is from this gene that FAD2-1Δ was derived more recently. Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

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Section: Discussionsupporting

confidence: 63%

A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.)

Rapson

Okada

et al. 2015

Front. Plant Sci.

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“…Thus, genetic improvement of safflower through marker-assisted breeding and genetic linkage of traits has been very limited. García-Moreno et al (2011) andHamdan et al (2012) were able to construct genetic linkage maps for the Tph2 gene and the Ol locus, respectively, using two F2 mapping populations. Tang et al (2010) performed a cDNA-AFLP linkage analysis using 192 randomly F2 segregating populations, in order to map heat shock protein (HSP) genes.…”

Section: Gene Mapping and Qtls For Drought Tolerancementioning

confidence: 99%

Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

197

130

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Safflower is one of the oldest cultivated crops, usually grown at a small scale. Safflower is grown for flowers used for coloring, flavoring foods, dyes, medicinal properties, and livestock feed. Safflower is underutilized but gaining attention due to oil yield potential and the ability to grow under high temperatures, drought, and salinity. Salinity and drought have negative effects by disrupting the ionic and osmotic equilibrium of the plant cells. The stress signal is perceived by membranes then transduced in the cell to switch on the stress responsive genes. This review discusses on stress tolerance mechanisms in safflower. Strategies are proposed for enhancing drought and salt resistance in safflower.

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“…Knowles (1989) also reported that oleic acid content of olol genotype was usually 71-75% of total fatty acids. Hamdan et al (2012) showed the dominant role of the FAD2-1 gene and the involvement of at least one modifying gene with positive effect further increases oleic acid content in safflower. However, the complex role of modifying genes is still a matter of investigation.…”

Section: Discussionmentioning

confidence: 95%

“…The ol allele was found to be a defective microsomal oleate desaturase FAD2-1 with a single nucleotide deletion in the coding region that leads to premature termination of translation and subsequent nonsense-mediated mRNA decay of FAD2-1, a process that typically degrades transcripts containing a premature termination codon (Guan et al, 2012;Liu et al, 2013). Hamdan et al (2012) described a SSR-based molecular marker for the OL locus.…”

Section: Discussionmentioning

confidence: 99%