Mapping of murine Interferon-α genes to chromosome 4

Kelley, Kevin; Kozak, Christine A.; Dandoy, F; Sor, Frédéric; Skup, Daniel; Windass, John D.; DeMaeyer-Guignard, J; Pitha, Paula M.; Demaeyer, E. M.

doi:10.1016/0378-1119(83)90188-9

Cited by 48 publications

(39 citation statements)

References 28 publications

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“…Thus, resistant C57 mice produced higher levels of interferon in response to the viruses than susceptible BALB/c mice and high and low interferon induction segregated between the male progeny of reciprocal crosses, with high levels occurring when the X chromosome was of C57 origin. Also very early (2 to 3 h) interferon production in mice in response to Newcastle disease virus (NDV) has been found to be influenced by an X-linked locus , although the If-1 locus controlling late (6 to 9 h) interferon production to this virus has been mapped on chromosome 3 (Mobraaten et al, 1984), and the structural genes for ct interferon have been mapped on chromosome 4 (Kelley et al, 1983;Lovett et al, 1984). It thus appears that the genetic basis for the magnitude of interferon levels in vivo is very complex , and that X-linked loci are involved.…”

Section: Discussionmentioning

confidence: 99%

X-Linkage of the Early in vitro /beta Interferon Response of Mouse Peritoneal Macrophages to Herpes Simplex Virus Type 2

Ellermann-Eriksen

Liberto

Iannello

et al. 1986

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

X-Linkage of the Early in vitro /beta Interferon Response of Mouse Peritoneal Macrophages to Herpes Simplex Virus Type 2

Ellermann-Eriksen

Liberto

Iannello

et al. 1986

Journal of General Virology

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“…Currently, two different subtypes of murine IFN-a have been described: IFN-a1 and -a2, and our probe corresponds to a2 (1,16). There is -90% homology between the sequence of IFN-a1 and -a2.…”

Section: Discussionmentioning

confidence: 99%

“…The recombinant plasmids pMIF 1204 (carrying a partial cDNA for murine IFN-a2) and pM l3-3 (carrying the complete nucleotide sequence for murine IFN-f3) have been described (1,2). After digestion with Pst I, the plasmids were electrophoresed through 1% agarose slab gels and the inserts [820 base pairs (bp) for IFN-a and 680 bp for IFN-,B1 were recovered from the gel slices by electroelution and chromatography on DE-52 columns.…”

Section: Methodsmentioning

confidence: 99%

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Identification of individual interferon-producing cells by in situ hybridization.

Zawatzky¹,

Maeyer²,

Maeyer‐Guignard³

1985

Proc. Natl. Acad. Sci. U.S.A.

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Individual interferon (IFN)-producing cells were identified by hybridization in situ followed by autoradiography. cDNAs corresponding to murine IFN-a and murine IFN-P labeled by nick-translation to high specific activity (24 x 10 dpm/,ug) with a-35S-labeled dATP were used as probes for hybridization with IFN mRNA in mouse C-243 cells induced with Newcastle disease virus. Control experiments with non-induced cells or with non-IFN-related labeled DNA monitored the specificity of the autoradiographic signal. Under optimal conditions of IFN induction, between 15% and 40% of the cells gave a hybridization signal with a mixture of IFN-a and -P probes. Differential hybridization with either the IFNa or -13 probe or a mixture of both, at three different time intervals after induction, revealed that only a small fraction of cells had detectable amounts of IFN-a mRNA, whereas in the majority of the positive cells IFN-(3 mRNA was present.Studies on the genetics and on the cellular origin of interferon (IFN) production have suffered from the limitation that the results represented average values obtained from cultures or from cell populations in vivo, because there was no method to identify and characterize individual IFN-producing cells at the same time. To be able to study IFN production at the single cell level is of obvious importance, because it can provide answers to several questions-for example, the number of IFN-producing cells in a given culture system or the nature and the number of IFN-producing cells in vivo as a function of the inducing agent. In the present study, we have used cDNA probes of murine IFN-a and -,B to develop a method for detecting the presence of specific mRNA by in situ hybridization. This method makes it possible to distinguish individual IFN-producing cells that can, at the same time, be stained for morphological identification. MATERIALS AND METHODSLabeling of cDNA Probes. The recombinant plasmids pMIF 1204 (carrying a partial cDNA for murine IFN-a2) and pM l3-3 (carrying the complete nucleotide sequence for murine IFN-f3) have been described (1, 2). After digestion with Pst I, the plasmids were electrophoresed through 1% agarose slab gels and the inserts [820 base pairs (bp) for IFN-a and 680 bp for IFN-,B1 were recovered from the gel slices by electroelution and chromatography on DE-52 columns. The inserts were labeled to high specific activity (2-4 x 108 dpm/,ug) with a-35S-labeled dATP (>1000 Ci/mmol; 1 Ci = 37 GBq; New England Nuclear) in a nick-translation reaction essentially as described by Haase et al. (3). Briefly, a 25-,ul reaction mixture contained 400 ng of DNA template/50 mM Tris-HCl, pH 7.4/10 mM MgCl/1 mM dithiothreitol/50,ug of nuclease-free bovine serum albumin per ml (Bethesda Research Laboratories)/30 ,uM of each dCTP, dGTP, and dTTP/250 ,JCi of a-35S-labeled dATP/5 units of DNA polymerase 1/100 pg of DNase I. The mixture was incubated at 15TC for 2 or 4 hr and incorporation of labeled nucleotides was determined by trichloroacetic acid precipitation in the presen...

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“…Eight of these [~1, ~2, c~4, c~5, ~6T, c~6P, ~A (~7) and ~9] encode biologically active proteins (Shaw et al, 1983;Daugherty et al, 1984;Zwarthoff et al, 1985;Kelley & Pitha, 1985 a;Self & DeMaeyer-Guignard, 1986;Kelley et al, 1986) and the other is a pseudo-gene (LeRoscouet et al, 1985). Results so far show that all mouse (Mu) IFN-~ genes are clustered on chromosome 4 (Kelley et al, 1983;Lovett et al, 1984;Dandoy et al, 1984Dandoy et al, , 1985Van der Korput et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Structure-Function Analysis of Mouse Interferon Alpha Species: MuIFN- 10, a Subspecies with Low Antiviral Activity

Trapman

Heuvel

Jonge

et al. 1988

Journal of General Virology

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Mapping of murine Interferon-α genes to chromosome 4

Cited by 48 publications

References 28 publications

X-Linkage of the Early in vitro /beta Interferon Response of Mouse Peritoneal Macrophages to Herpes Simplex Virus Type 2

X-Linkage of the Early in vitro /beta Interferon Response of Mouse Peritoneal Macrophages to Herpes Simplex Virus Type 2

Identification of individual interferon-producing cells by in situ hybridization.

Structure-Function Analysis of Mouse Interferon Alpha Species: MuIFN- 10, a Subspecies with Low Antiviral Activity

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