Mapping of putative ether-anesthesia resistance gene using C57BL/6J and MSM/Ms mouse strains

Megumi, Tsuneo; Song, Chang-Woo; Park, Yeong-Gwan; Tanaka, Yoshiharu; Uchida, Ichirō; Okumoto, Masaaki

doi:10.1007/s00540-004-0251-7

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Recent guidelines put some restrictions in the use of ether for rapid anesthesia of rodents and other laboratory animals (Close et al, ). Animal strains resistant to ether have also been described (Megumi et al, ).…”

Section: Commentarymentioning

confidence: 99%

Assessment of Intestinal Peristalsis in Vitro

Pozzoli

Poli

2012

CP Toxicology

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The protocol detailed in this unit is designed to assess intestinal peristaltic motility in the isolated small intestine in vitro and to measure the effects of drugs able to interfere with gut propulsive activity. The procedure is based on Trendelenburg's classic technique, described at the beginning of the 20th century in the isolated guinea pig ileum and, later on, extended to other intestinal preparations from the same animal and other animal species. This unit illustrates the basic procedures for setting up the intestinal preparation, recording peristalsis under near-physiologic conditions, and testing the pharmaco-toxicological effects of drugs and pollutants on the contractile behavior of the gut wall. The protocol allows evaluating the action of drugs affecting sensory and/or motor neurons of the enteric nervous system and how these neurons control the development of the motor program of the gut wall. This model can be exploited to investigate novel compounds undergoing preclinical development and both inhibitors and stimulants of gastrointestinal peristaltic activity, as well as environmental or alimentary pollutants, like xenobiotics and naturally-occurring toxins, endowed with noxious activity with regard to digestive functions.

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Section: Commentarymentioning

confidence: 99%

Assessment of Intestinal Peristalsis in Vitro

Pozzoli

Poli

2012

CP Toxicology

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“…As an optimum, experiments with ether should be performed by placing the glass bell (with the animal inside) under a laboratory fume hood, thus limiting vapor spread into the room. Thirdly, a sort of resistance to diethyl ether anesthesia has been described in some mice strains (Megumi et al, 2004). Swiss mice are usually sensitive to ether, so the problem is only theoretical when using this animal strain.…”

Section: Critical Parameters and Troubleshootingmentioning

confidence: 99%

Assessment of Gastrointestinal Propulsive Activity Using Three Different Models of Peristalsis In Vivo in the Mouse

Poli

Pozzoli

2010

CP Toxicology

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The protocols described in this unit are designed to assess the acute effects of drugs on the propulsive activity of the gastrointestinal muscles in the conscious mouse. These protocols are currently applied to investigate the pharmacological activity of novel compounds undergoing preclinical development and to obtain predictive data needed to advance drugs into clinical trials. Moreover, these methods could be useful in evaluating the functional toxicity by environmental or alimentary pollutants, like xenobiotics and naturally occurring toxins endowed with noxious activity in the control of physiologic peristalsis. The three models detailed-the measurement of gastric emptying, ileal transit, and colonic propulsion-are substantially non-invasive and do not require analgesic pretreatments or the induction of general anesthesia. In contrast to an in vitro approach, these in vivo studies provide a unified understanding of drug effects on gut functionality, in particular when the central nervous system, the extrinsic nerves, or the (neuro)endocrine system is targeted by the test drugs.

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Mouse Chromosome 7 Harbors a Quantitative Trait Locus for Isoflurane Minimum Alveolar Concentration

Cascio

Xing

Gong

et al. 2007

Anesthesia &Amp; Analgesia

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Mapping of putative ether-anesthesia resistance gene using C57BL/6J and MSM/Ms mouse strains

Abstract: Microsatellite linkage analyses, including QTL analysis, determined the location of the ether-resistance gene, Etan1, within a narrow range. Our findings should be helpful for further experiments, such as cloning of the gene governing the sensitivity to ether anesthesia in mice.

Cited by 3 publications

References 23 publications

Assessment of Intestinal Peristalsis in Vitro

Assessment of Intestinal Peristalsis in Vitro

Assessment of Gastrointestinal Propulsive Activity Using Three Different Models of Peristalsis In Vivo in the Mouse

Mouse Chromosome 7 Harbors a Quantitative Trait Locus for Isoflurane Minimum Alveolar Concentration

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