Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.)

Qu, Yanzhi; Wu, Penghao; Ren, Jiaojiao; Liu, Zonghua; Tang, Jihua; Lübberstedt, Thomas; Li, Haochuan; Chen, Shaojiang

doi:10.1371/journal.pone.0228411

Cited by 6 publications

(6 citation statements)

References 37 publications

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“…In the automated NMR system based on oil content, the CDR for haploid kernels was 94% [ 41 ]. EmA kernels are a common phenomenon in in vivo haploid induction [ 21 , 47 , 49 ], and EmA kernels with lower oil contents are usually mixed with haploid kernels when a single threshold for oil content is used in sorting. In the present study, the percent of EmA to haploid kernels in maize hybrids and inbred lines were 53.6% and 87.4%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The haploid induction rates (HIRs) of CAUHOI and CHOI2 are ~ 2.0% and ~ 7%, respectively [ 38 , 56 ], and both inducers were developed at China Agricultural University. The maize inducer YHI-1 with a HIR > 10% was developed at Henan Agricultural University [ 47 , 57 ]. Three maize hybrids, 4F1/Zheng58, Mo17/L119A, and Xun9058/Dan598, were used as females in crosses with each inducer, and nine inbred lines (TieC8605-2, Zheng58; Lx9801, K12, Dan598, Zheng22, Yuzi87-1, P138, and L217) were selected for use as females to cross with CHOI2 (Table 6 ).…”

Section: Methodsmentioning

confidence: 99%

“…At present, oil content analysis is usually employed with a single threshold to discriminate haploid kernels using NMR. However, EmA kernels are produced along with haploid and diploid kernels on the same ears during in vivo haploid induction [ 45 – 47 ]. There is a highly positive correlation between the haploid induction rate (HIR) and the embryo abortion rate (EmAR) [ 47 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, EmA kernels are produced along with haploid and diploid kernels on the same ears during in vivo haploid induction [ 45 – 47 ]. There is a highly positive correlation between the haploid induction rate (HIR) and the embryo abortion rate (EmAR) [ 47 , 48 ]. The haploid and EmA kernels, both of which have lower oil content than diploid kernels, are usually mixed together during the process of haploid sorting by NMR.…”

Section: Introductionmentioning

confidence: 99%

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Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds

Liu

Zhang

et al. 2021

Plant Methods

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Background Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prerequisite for the large-scale application of this technology. At present, the automatic haploid sorting based on nuclear magnetic resonance (NMR) using a single threshold method has been realized. However, embryo-aborted (EmA) kernels are usually produced during in vivo haploid induction, and both haploids and EmA kernels have lower oil content and are separated together using a single threshold method based on NMR. This leads to a higher haploid false discrimination rate (FDR) and requires secondary manual sorting to select the haploid kernels from the mixtures, which increases the sorting cost and decreases the haploid sorting efficiency. In order to improve the correct discrimination rate (CDR) in sorting haploids, a method to distinguish EmA kernels is required. Results Single kernel weight and oil content were measured for the diploid, haploid, and EmA kernels derived from three maize hybrids and nine inbred lines by in vivo induction. The results showed that the distribution of oil content showed defined boundaries between the three types of kernels, while the single kernel weight didn't. According to the distribution of oil content in the three types of kernels, a double-threshold method was proposed to distinguish the embryo-aborted kernels, haploid and diploid kernels based on NMR and their oil content. The double thresholds were set based on the minimum oil content of diploid kernels and the maximum content of EmA kernels as the upper and lower boundary values, respectively. The CDR of EmA kernels in different maize materials was > 97.8%, and the average FDR was reduced by 27.9 percent. Conclusions The oil content is an appropriate indicator to discriminate diploid, haploid and EmA kernels. An oil content double-threshold method based on NMR was first developed in this study to identify the three types of kernels. This methodology could reduce the FDR of haploids and improve the sorting efficiency of automated sorting system. Thus, this technique represents a potentially efficient method for haploid sorting and provides a reference for the process of automated sorting of haploid kernels with high efficiency using NMR.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds

Liu

Zhang

et al. 2021

Plant Methods

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“…In addition, the introduction of the matl/nld/zmpla1 mutation was associated with a dramatic decrease in the seed setting rate. Hence, future research into the mechanism(s) of haploid induction will be necessary to uncouple or reduce the haploid induction and fecundity-loss phenotypes 62 , thus enabling this strategy to become commercially viable.…”

Section: Haploid Induction Systems As a Building Stone For The Engineering Of Cropsmentioning

confidence: 99%

Puzzling out plant reproduction by haploid induction for innovations in plant breeding

Jacquier¹,

Gilles²,

Pyott³

et al. 2020

Nat. Plants

127

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Mixing maternal and paternal genomes in embryos is not only responsible for the evolutionary success of sexual reproduction but a cornerstone of plant breeding. However, once an interesting gene combination is obtained, further genetic mixing is problematic. To rapidly fix genetic information, doubled-haploid plants can be produced: haploid embryos having solely the genetic information from one parent are allowed to develop and chromosome doubling generates fully homozygous plants. A powerful path to doubledhaploids production is based on haploid inducer lines. A simple cross between a haploid inducer line and the line with gene combinations to be fixed will trigger haploid embryo development. However the exact mechanism behind in-planta haploid induction remains an enduring mystery. The recent discoveries of molecular actors triggering haploid induction in the maize crop and the model Arabidopsis thaliana pinpoint an essential role of processes related to gamete development, gamete interactions and genome stability. These findings enabled translation of haploid induction capacity to other crops, and the use of haploid inducer lines to deliver genome editing machinery into various crop varieties. These recent advances not only hold promise for the next generations of plant breeding strategies, but it also provides a deeper insight into the fundamental bases of sexual reproduction in plants.

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Development of high-oil maize haploid inducer with a novel phenotyping strategy

Liu

Chen

et al. 2022

The Crop Journal

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Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.)

Cited by 6 publications

References 37 publications

Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds

Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds

Puzzling out plant reproduction by haploid induction for innovations in plant breeding

Development of high-oil maize haploid inducer with a novel phenotyping strategy

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