Mapping of quantitative trait loci conferring resistance to brown spot in flue‐cured tobacco (<i>Nicotiana tabacum</i> L.)

Tong, Zhijun; Jiao, Tianlei; Wang, Fengqing; Li, Meiyun; Leng, Xiaodong; Gao, Yulong; Li, Yongping; Xiao, Bingguang; Wu, Weiren

doi:10.1111/j.1439-0523.2011.01940.x

Cited by 20 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that brown spot infestations increase as flue‐cured tobacco grows. These results are similar to the Tong et al (2012) findings that foliar diseases on tobacco such as brown spot occur with greater frequency at later growth stages. The ANOVA results also showed that both the incidence and severity index of brown spot were significantly affected by year (Table 2).…”

Section: Resultssupporting

confidence: 90%

Effects of Relay Cropping of Corn on the Productivity and Quality of Flue‐Cured Tobacco

Zhou

Yin

et al. 2014

Agronomy Journal

View full text Add to dashboard Cite

Relay cropping timing has been frequently reported to in uence ue-cured tobacco (Nicotiana tabacum L.) production. However, the optimal planting time for a relay corn (Zea mays L.) crop on ue-cured tobacco elds is unknown in terms of tobacco health, yield, quality, and economic value. A eld experiment was conducted with 10, 17, 24, and 31 d a er ue-cured tobacco apex excision as the relay cropping timings of corn, and tobacco monoculture as the control at Chuxiong, Yunnan, China in 2010 and 2011. e ve treatments were repeated three times in a randomized complete block design. e occurrence of brown spot (Alternaria alternata), plant biomass, plant N and K nutrition, yield, chemical composition, smoking quality, and economic value of ue-cured tobacco were a ected by year. Relay cropping 17 d a er tobacco apex excision resulted in better chemical composition and smoking quality. e incidence and severity index of brown spot, plant biomass, plant N and K nutrition, yield, chemical composition, smoking quality, and economic value of tobacco were not signi cantly a ected by the relay cropping timing of corn, indicating that relay cropping of corn at a later growth stage of ue-cured tobacco has no negative e ect on tobacco production while the relay corn crop may bring additional income to tobacco growers. In conclusion, if the production of relay corn is pro table, relay cropping of corn a er ue-cured tobacco apex excision could become a recommended cropping system in Yunnan and other regions with similar environmental conditions. 0.001 probability level. † The perfect total score is 100, composed of aroma type 15, aroma amount 20, residue taste 25, undesirable smell 18, irritation 12, combustibility 5, and ash color 5. ‡ ns, not significant at 0.05 probability level. § Means in a column within the years averaged over the treatments followed by the same letter are not significantly different at P = 0.05 according to the Fisher's protected LSD in the SAS Procedure Mixed.

show abstract

Section: Resultssupporting

confidence: 90%

Effects of Relay Cropping of Corn on the Productivity and Quality of Flue‐Cured Tobacco

Zhou

Yin

et al. 2014

Agronomy Journal

View full text Add to dashboard Cite

show abstract

“…Our comparison of distribution revealed that SSRs in Nicotiana are mainly located in the regions between genes. This observation may partly explain the low rate (~ 4%) of polymorphism identified during Nicotiana SSR marker screening in an earlier study [ 39 ] that used genic sequences as the starting point for SSR discovery.…”

Section: Discussionmentioning

confidence: 99%

Comparative genome-wide characterization leading to simple sequence repeat marker development for Nicotiana

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundSimple sequence repeats (SSRs) are tandem repeats of DNA that have been used to develop robust genetic markers. These molecular markers are powerful tools for basic and applied studies such as molecular breeding. In the model plants in Nicotiana genus e.g. N. benthamiana, a comprehensive assessment of SSR content has become possible now because several Nicotiana genomes have been sequenced. We conducted a genome-wide SSR characterization and marker development across seven Nicotiana genomes.ResultsHere, we initially characterized 2,483,032 SSRs (repeat units of 1–10 bp) from seven genomic sequences of Nicotiana and developed SSR markers using the GMATA® software package. Of investigated repeat units, mono-, di- and tri-nucleotide SSRs account for 98% of all SSRs in Nicotiana. More complex SSR motifs, although rare, are highly variable between Nicotiana genomes. A total of 1,224,048 non-redundant Nicotiana (NIX) markers were developed, of which 99.98% are novel. An efficient and uniform genotyping protocol for NIX markers was developed and validated. We created a web-based database of NIX marker information including amplicon sizes of alleles in each genome for downloading and online analysis.ConclusionsThe present work constitutes the first deep characterization of SSRs in seven genomes of Nicotiana, and the development of NIX markers for these SSRs. Our online marker database and an efficient genotyping protocol facilitate the application of these markers. The NIX markers greatly expand Nicotiana marker resources, thus providing a useful tool for future research and breeding. We demonstrate a novel protocol for SSR marker development and utilization at the whole genome scale that can be applied to any lineage of organisms.The Tobacco Markers & Primers Database (TMPD) is available at http://biodb.sdau.edu.cn/tmpd/index.htmlElectronic supplementary materialThe online version of this article (10.1186/s12864-018-4878-4) contains supplementary material, which is available to authorized users.

show abstract

“…Polymerase chain reactions (PCR) were performed for rolB and rolC using specific primers recognizing rolB (5′-GCTCTTGCAGTGCTAGATTT-3′; 5′-GAAGGTGCAAGCTACCTCTC-3′) and rolC (5′-CTCCTGACATCAAACTCGTC-3′; 5′-TGCTTCGAGTTATGGGTACA-3′). The amplification system and procedure were the same as in Tong et al ( 2012 ), except for the primer annealing temperature, which was 58°C.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of Salicylic Acid Treatment in Rehmannia glutinosa Hairy Roots Using RNA-seq Technique for Identification of Genes Involved in Acteoside Biosynthesis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Rehmannia glutinosa is a common bulk medicinal material that has been widely used in China due to its active ingredients. Acteoside, one of the ingredients, has antioxidant, antinephritic, anti-inflammatory, hepatoprotective, immunomodulatory, and neuroprotective effects, is usually selected as a quality-control component for R. glutinosa herb in the Chinese Pharmacopeia. The acteoside biosynthesis pathway in R. glutinosa has not yet been clearly established. Herein, we describe the establishment of a genetic transformation system for R. glutinosa mediated by Agrobacterium rhizogenes. We screened the optimal elicitors that markedly increased acteoside accumulation in R. glutinosa hairy roots. We found that acteoside accumulation dramatically increased with the addition of salicylic acid (SA); the optimal SA dose was 25 μmol/L for hairy roots. RNA-seq was applied to analyze the transcriptomic changes in hairy roots treated with SA for 24 h in comparison with an untreated control. A total of 3,716, 4,018, and 2,715 differentially expressed transcripts (DETs) were identified in 0 h-vs.-12 h, 0 h-vs.-24 h, and 12 h-vs.-24 h libraries, respectively. KEGG pathway-based analysis revealed that 127 DETs were enriched in “phenylpropanoid biosynthesis.” Of 219 putative unigenes involved in acteoside biosynthesis, 54 were found to be up-regulated at at least one of the time points after SA treatment. Selected candidate genes were analyzed by quantitative real-time PCR (qRT-PCR) in hairy roots with SA, methyl jasmonate (MeJA), AgNO3 (Ag+), and putrescine (Put) treatment. All genes investigated were up-regulated by SA treatment, and most candidate genes were weakly increased by MeJA to some degree. Furthermore, transcription abundance of eight candidate genes in tuberous roots of the high-acteoside-content (HA) cultivar QH were higher than those of the low-acteoside-content (LA) cultivar Wen 85-5. These results will pave the way for understanding the molecular basis of acteoside biosynthesis in R. glutinosa, and can serve as a basis for future validation studies.

show abstract

Mapping of quantitative trait loci conferring resistance to brown spot in flue‐cured tobacco (Nicotiana tabacum L.)

Cited by 20 publications

References 21 publications

Effects of Relay Cropping of Corn on the Productivity and Quality of Flue‐Cured Tobacco

Effects of Relay Cropping of Corn on the Productivity and Quality of Flue‐Cured Tobacco

Comparative genome-wide characterization leading to simple sequence repeat marker development for Nicotiana

Transcriptome Analysis of Salicylic Acid Treatment in Rehmannia glutinosa Hairy Roots Using RNA-seq Technique for Identification of Genes Involved in Acteoside Biosynthesis

Contact Info

Product

Resources

About