Mapping of quantitative‐trait loci by means of marker genes in F2 generations of Wild boar, Pietrain and Meishan pigs

Geldermann, H.; Müller, Elisabeth; Beeckmann, Petra; Knorr, C.; Yue, Gen Hua; Moser, G.

doi:10.1111/j.1439-0388.1996.tb00629.x

Cited by 50 publications

(19 citation statements)

References 6 publications

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“…Three-generation pedigrees of Meishan × Piétrain, Wild Boar × Meishan, and Wild Boar × Piétrain established at the University of Hohenheim (Geldermann et al, 1996) were used to study polymorphism and inheritance. CRI-MAP, version 2.4 software package was employed for linkage mapping the Hohenheim pedigrees.…”

Section: Linkage Analysismentioning

confidence: 99%

Porcine PPARGC1A (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping

Jacobs

Rohrer

Poucke

et al. 2005

Cytogenet Genome Res

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We report here the characterisation of porcine PPARGC1A. Primers based on human PPARGC1A were used to isolate two porcine BAC clones. Porcine coding sequences of PPARGC1A were sequenced together with the splice site regions and the 5′ and 3′ regions. Using direct sequencing nine SNPs were found. Allele frequencies were determined in unrelated animals of five different pig breeds. In the MARC Meishan-White Composite resource population, the polymorphism in exon 9 was significantly associated with leaf fat weight. PPARGC1A has been mapped by FISH to SSC8p21. A (CA)_n microsatellite (SGU0001) has been localised near marker SWR1101 on chromosome 8 by RH mapping and at the same position as marker KS195 (32.5 cM) by linkage mapping. The AseI (nt₈₅₇, Asn/Asn₄₈₉) polymorphism in exon 8 was used to perform linkage analysis in the Hohenheim pedigrees and located the gene in the same genomic region. Transcription of the gene was detected in adipose, muscle, kidney, liver, brain, heart and adrenal gland tissues, which is in agreement with the function of PPARGC1A in adaptive thermogenesis.

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Section: Linkage Analysismentioning

confidence: 99%

Porcine PPARGC1A (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping

Jacobs

Rohrer

Poucke

et al. 2005

Cytogenet Genome Res

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“…Three F 2 populations were constructed by crossing diverse pig breeds: European Wild Boar (W), Chinese Meishan (M) and Pietrain (P) [2,7] . The Wild Boar × Pietrain pedigree (W × P) comprised one male European Wild boar and nine female Pietrain founders, two F 1 males and 28 …”

Section: Animals and Traitsmentioning

confidence: 99%

“…are quantitative in nature. The use of mapped DNA markers provides a tool for dissecting the genetic basis of complex quantitative traits [1,2] . The Meishan pig, a Chinese domestic breed, is prolific but has slow growth and excessive fat.…”

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confidence: 99%

“…The Meishan's slow growth and excessive fat make it difficult to develop both a prolific and lean synthetic line involving the Meishan breed. Although this breed has been widely studied [2][3][4][5][6] , the genetic mechanisms underlying the phenotype of extremely high fat content in the carcass have not yet been clearly explained. In this study we report the finding of QTL alleles on chromosome 7 from fatty Meishan pigs, which substantially reduce fat deposition.…”

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confidence: 99%

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QTL alleles on chromosome 7 from fatty Meishan pigs reduce fat deposition

Yue

Beeckmann

Moser

et al. 2003

Sci. China Ser. C.-Life Sci.

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For detecting QTL in the whole swine genome, 1068 pigs from three F2 populations constructed by crossing European Wild boar and Pietrain (W x P), Meishan and Pietrain (M x P), and Wild Boar and Meishan (W x M) were genotyped for genetic markers evenly spaced at approximately 20 cM intervals. AQTL analysis was performed using a least-squares method. Here the results of the QTL analysis on the porcine chromosome 7 are presented. QTL for carcass composition (e.g. head weight, carcass length, backfat depth, abdominal fat and bacon meat) were mapped in the chromosomal region CYPA/CYPD-TNFB-S0102 in M x P and W x M, but not in W x P. The QTL explained 5.3%-27.2% of the F2 phenotypic variance in the two F2 populations. Most traits affected by the mapped QTL were related to carcass fatness. The mode of gene action of QTL was additive. Surprisingly, in contrast to the parental phenotype, the QTL alleles from fatty Meishan were associated with thinner backfat than Pietrain and Wild Boar alleles, suggesting that the genome of the fatty Meishan pig contains genes which can reduce fat content of carcass substantially.

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“…Several Meishan pigs were exported to the USA and Europe in 1980s, and had been used in breeding programs (Ducos et al, 1992) and in pig genome mapping projects (Geldermann et al, 1996;Rohrer et al, 1996). A study on QTL mapping showed that the Meishan pig possessed QTL alleles which could reduce fat content in carcass, despite its fat appearance (Yue et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular genetic analysis of the Chinese Erhualian pig breed

Yue¹,

Gl²

2004

SA J. An. Sci.

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The Chinese Erhualian is one of the most prolific pig breeds in the world, but it is in danger of being replaced by other exotic pig breeds because of its slow growth rate and high fat content in the body. To obtain some genetic information for conservation, we analysed the Erhualian pigs by using a PCR-RFLP for the calcium-release-channel (CRC) gene, nine polymorphic microsatellites and the complete mtDNA D-loop sequences, and compared these data with those from other pig breeds from Europe and Asian. The PCR-RFLP analysis of the CRC gene showed that the frequency of the C allele associated with stress resistance was 100% in the Erhualian pigs. Neighbour-Joining trees constructed on the basis of mtDNA D-loop sequences and the microsatellite analysis clearly showed that the Erhualian pigs were located in a separate branch. These data suggest that the Erhualian pigs are different from other breeds. Microsatellite analysis showed that the average allele number (5.3/locus) in the Erhualian pig was intermediate as compared with that (4.8-7.0/locus) in the three European pig breeds. The expected heterozygosity was higher in the Erhualian pig (0.78) than that in these European pig breeds (0.59-0.72), whereas the observed heterozygosity was higher in the European breeds (0.51-0.64) than in the Erhualian pig (0.46). In the Erhualian pig, the fixation index (F IS ) was as high as 0.41. These data suggest a high level of inbreeding and/or subpopulation in the Erhualian pigs. For conservation of the germplasm in the Erhualian pigs, it is necessary to take measures to reduce inbreeding and/or subpopulation.

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Mapping of quantitative‐trait loci by means of marker genes in F₂ generations of Wild boar, Pietrain and Meishan pigs

Cited by 50 publications

References 6 publications

Porcine <i>PPARGC1A</i> (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping

Porcine <i>PPARGC1A</i> (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping

QTL alleles on chromosome 7 from fatty Meishan pigs reduce fat deposition

Molecular genetic analysis of the Chinese Erhualian pig breed

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