Mapping of quantitative trait loci for tuber starch and leaf sucrose contents in diploid potato

Abstract

Key messageMost QTL for leaf sucrose content map to positions that are similar to positions of QTL for tuber starch content in diploid potato.AbstractIn the present study, using a diploid potato mapping population and Diversity Array Technology (DArT) markers, we identified twelve quantitative trait loci (QTL) for tuber starch content on seven potato chromosomes: I, II, III, VIII, X, XI, and XII. The most important QTL spanned a wide region of chromosome I (42.0–104.6 cM) with peaks at 63 and 84 cM which explained 17.6 and 19.2 % of the phenotypic variation, respectively. ADP-glucose pyrophosphorylase (AGPase) is the key enzyme for starch biosynthesis. The gene encoding the large subunit of this enzyme, AGPaseS-a, was localized to chromosome I at 102.3 cM and accounted for 15.2 % of the variance in tuber starch content. A more than 100-fold higher expression of this gene was observed in RT-qPCR assay in plants with the marker allele AGPaseS-a1334. This study is the first to report QTL for sucrose content in potato leaves. QTL for sucrose content in leaves were located on eight potato chromosomes: I, II, III, V, VIII, IX, X and XII. In 5-week-old plants, only one QTL for leaf sucrose content was detected after 8 h of darkness; four QTL were detected after 8 h of illumination. In 11-week-old plants, 6 and 3 QTL were identified after dark and light phases, respectively. Of fourteen QTL for leaf sucrose content, eleven mapped to positions that were similar to QTL for tuber starch content. These results provide genetic information for further research examining the relationships between metabolic carbon molecule sources and sinks in potato plants.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-015-2615-9) contains supplementary material, which is available to authorized users.

Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

“…The DArT map for mch (Śliwka et al 2012a) includes 846 DArT markers and the map for S. ruiz - ceballosii contained 1603 DArT markers (Śliwka et al 2012b). Next three maps were constructed in IHAR–PIB, Młochów and consisted of 1597, 1420, and 1370 DArT markers, respectively (Sołtys-Kalina et al 2015; Śliwka et al 2016; data unpublished, personal communication with Agnieszka Hara-Skrzypiec). The chromosomes richest in markers were chromosomes I (133–230 markers) and II (75–260 markers) in all six analyzed DArT maps.…”

“…The chromosomes richest in markers were chromosomes I (133–230 markers) and II (75–260 markers) in all six analyzed DArT maps. The smallest number of markers was observed on chromosome IV in two maps (Sołtys-Kalina et al 2015; data unpublished, personal communication with Agnieszka Hara-Skrzypiec) (29 and 48 markers), and the map for S. ruiz - ceballosii (74), on chromosome V (86) in the third map (Śliwka et al 2016), on chromosome VII (28) in the mch map, and on chromosome XII (36) in the physical map (Sharma et al 2013). The results of analysis of mch (+) tbr somatic hybrid genomes corresponded well with these data.…”

“…Markers were selected if they were polymorphic between mch and tbr parents of each fusion combination and passed the following quality control parameters: p value, call rate, PIC and discordance. Localization of individual markers to the appropriate chromosome was performed based on comparison with DArT maps of diploid potato species: S. phureja (Sharma et al 2013), mch (Śliwka et al 2012a), S. ruiz - ceballosii (Śliwka et al 2012b) and three diploid hybrids of tbr (Sołtys-Kalina et al 2015; Śliwka et al 2016; data unpublished, personal communication with Agnieszka Hara-Skrzypiec). To determine the nuclear genome composition of mch (+) tbr somatic hybrids, preserved and deleted markers were described.…”

“…DArT linkage maps of potato were created for Solanum   ×   michoacanum ( mch ) (Śliwka et al 2012a), Solanum ruiz - ceballosii (Śliwka et al 2012b) and a doubled haploid DM1–3 of Solanum phureja , for which a physical map is available, too (Sharma et al 2013). Three additional DArT maps of diploid potato (Sołtys-Kalina et al 2015; Śliwka et al 2016; data unpublished, personal communication with Agnieszka Hara-Skrzypiec) are available. Using the DArT method, it is possible to generate several hundred to several thousand markers in a single assay (Kilian et al 2005; Wittenberg et al 2005).…”

TARGET GENES FOR DEVELOPMENT OF POTATO (Solanum tuberosum L.)CULTIVARS WITH DESIRED STARCH PROPERTIES (review)

“…Using the method of interval mapping of genomes in a population of diploid potatoes, it was shown that starch phosphorylation is regulated and/or controlled by five quantitative trait loci (QTL) on chromosomes 2, 5 and 9, and the content of amylose by six loci on chromosomes 2, 3, 5, 7 and 10. Similarly, loci controlling the starch grain size, the starch content of potato tuber and the temperature of starch gelatinization were discovered [45,46]. Many of the identified QTLs coincided by their localization with the known genes that encode the enzymes of starch biosynthesis, but, in addition, loci have also been discovered in which no one of the starch biosynthetic genes was previously mapped.…”