Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice (Oryza sativa L.)

Abstract

Rice black‐streaked dwarf virus disease (RBSDVD), transmitted by small brown planthopper (SBPH, Laodelphax striatellus), causes serious loss in rice production. Breeding resistant cultivars are one of the most effective strategies to control the virus disease and its vector. By both natural inoculations in the field and modified seedling‐box screening test in the glasshouse, an indica variety WR24 showed high resistance to RBSDVD and SBPH. An F2:3 population consisting of 153 lines derived from a cross between WR24 and a susceptible japonica variety Suyunuo was used for quantitative trait loci (QTL) analysis of RBSDVD and SBPH resistance. The linkage map consisting of 130 SSR markers was constructed with an average marker interval of 13.90 cM, spanning a total of 1890.9 cM. Totally, five QTLs for RBSDV resistance, viz. qRBSDV3WR24, qRBSDV6WR24, qRBSDV7WR24, qRBSDV9WR24 and qRBSDV11WR24, were detected on chromosomes 3, 6, 7, 9 and 11, with LOD scores of 2.7, 3.08, 3.13, 5.28 and 3.7, respectively. Meanwhile, three QTLs for SBPH resistance, including qSBPH5WR24, qSBPH7WR24 and qSBPH10WR24, were mapped on chromosomes 5, 7 and 10, with LOD scores of 2.18, 3.5 and 3.57, respectively. All resistant alleles were from WR24. Among these QTLs, qRBSDV7WR24, qSBPH5WR24 and qSBPH10WR24 were newly reported, and qSBPH10WR24 showed major effect that explained 17.9% of total phenotypic variance. The RBSDVD and SBPH resistance QTLs and the tightly linked DNA markers can be utilized in RBSDV and SBPH resistance breeding in rice.

SET Domain Group 703 Regulates Planthopper Resistance by Suppressing the Expression of Defense-Related Genes

“…To ensure effective and accurate scoring of all test materials, the seedlings of the susceptible control were allowed to die based on the bulk seedling test [ 52 ]. The resistance to SBPH of all test materials was assessed using a slightly modified seedling box screening test described by Xu [ 53 ]. In this test, approximately 30 seeds from each individual plant were planted in a soil-filled plastic pot measuring 5.8 cm × 6.0 cm, with a hole at the bottom.…”

Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

“…Three QTLs related to SBPH resistance (qSBPH2b, qSBPH3d, and qSBPH12a) were identified on chromosomes 2, 3, and 12 in the cultivar Mudgo, respectively (Duan Karim andPathak 1982 et al 2009). Additional QTLs for SBPH resistance were identified, including qSBPH2, qSBPH3, qSBPH8, and qSBPH11 on chromosome 2, 3, 8, and 11 in Kasalath, respectively; qSBPH2, qSBPH3, qSBPH5, qSBPH7, and qSBPH11 on chromosome 2, 3, 5, 7, and 11 in N22, respectively; qSBPH3d, qSBPH7a, and qSBPH12b on chromosome 3, 7, and 12 in Pf9279-4, respectively; qSBPH1, qSBPH5, qSBPH8, and qSBPH9 on chromosome 1, 5, 8, and 9 in 9194, respectively; and qSBPH5, qSBPH7, and qSBPH10 on chromosome 5, 7, and 10 in WR24, respectively (Duan et al 2010, Wang et al 2013b, Sun et al 2017, Xu et al 2018b, Table 1). Although individual QTLs have only small effects on SBPH resistance, it is useful to apply multiple QTLs for SBPH resistance breeding.…”

Rice black‐streaked dwarf virus: From multiparty interactions among plant–virus–vector to intermittent epidemics

“…To facilitate the use and transfer of host resistance in breeding programmes, recent QTL mapping or genome-wide association studies have identified genomic regions associated with RBSDV resistance and linked DNA markers (Feng et al, 2019;Xiao et al, 2019). Several QTLs on different chromosomes have been identified in japonica cultivar Koshihikari, and indica cultivars Minghui 63, WR24, Tetep, IR36, and 9194 (Wang et al, 2010;Li et al, 2013;Zhou et al, 2015a;Zhang et al, 2016;Sun et al, 2017;Xu et al, 2018).…”