Mapping of quantitative trait loci for seedling salt tolerance in maize

Luo, Meijie; Zhang, Yunxia; Chen, Kuan; Kong, Mengsi; Song, Wei; Lu, Bingyue; Shi, Yaxing; Zhao, Yanxin; Zhao, Jiuran

doi:10.1007/s11032-019-0974-7

Cited by 40 publications

(31 citation statements)

References 33 publications

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“…ZmNHX8 was upregulated in NaCl treatment, but ZmNHX4 , - 5 , and -11 were downregulated in root. NHX7/SOS1 is critical for excluding Na + from plant roots [51] and ZmNHX2 is associated with a major quantitative trait locus (QTL), qST1 , which confers salt tolerance on maize plants [52, 53]. Upregulated expressions of ZmNHX8 in our salinity treatment support the idea that their roles in response to salt stress may be conserved in maize.…”

Section: Discussionmentioning

confidence: 65%

Genome-wide identification, characterization, and expression analysis of the monovalent cation-proton antiporter superfamily in maize, and functional analysis of its role in salt tolerance

Kong

Luo

et al. 2020

Preprint

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BackgroundSodium toxicity and potassium insufficient are important factors affecting the growth and development of maize in saline soil. The monovalent cation proton antiporter (CPA) superfamily comprises Na+/H+ exchanger (NHX), K+ efflux antiporter (KEA), and cation/H+ exchanger (CHX) subfamily proteins, which play vital functions in maize salt tolerance.ResultsA total of 35 ZmCPA genes were identified in maize, and they were phylogenetically classified into 13 ZmNHXs, 16 ZmCHXs and 6 ZmKEAs. ZmCPA genes have a conserved gene structure, with the determined introns range from 11 to 25, 0 to 5 and 16 to 19 in ZmNHXs, ZmCHXs, ZmKEAs, respectively. All proteins have transmembrane domains, with an average transmembrane number of 8, 10, and 10 in ZmNHX, ZmCHX and ZmKEA proteins, respectively. Transient expression in maize protoplasts showed that ZmCHX16 and ZmNHX8 are located in the cell membrane. All ZmCHX subfamily genes showed lower expression compared to ZmNHX and ZmKEA subfamilies. Diverse expression in the 60 tissues and modulated expression in response to salt stress suggested ZmCPAs’ role in maize development and salt stress. Yeast complementary experiment revealed the function of ZmNHX8, ZmCHX8, -12, -14, -16 and ZmKEA6 in salt tolerance. Maize mutants zmnhx8 and zmkea6 further validated the important function of ZmNHX8 and ZmKEA6 in salt tolerance. Phosphorylation sites and cis-acting regulation elements analyses indicated that phosphorylation and transcriptional regulation may be involved in salt tolerance of ZmCPA genes.ConclusionsOur study provides comprehensive information about ZmCPA gene superfamily, which would be useful in their future functional characterization.

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Section: Discussionmentioning

confidence: 65%

Genome-wide identification, characterization, and expression analysis of the monovalent cation-proton antiporter superfamily in maize, and functional analysis of its role in salt tolerance

Kong

Luo

et al. 2020

Preprint

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“…In recent years, many QTLs related to the salt tolerance of major field crops such as rice, soybean, wheat, and maize, as well as vegetable crops such as cabbage and tomato have been reported [ 13 , 14 , 20 , 21 , 22 , 23 ]. But currently there are few reports on the mining of cucumber salt tolerant genes, and no QTL mapping studies have been carried out.…”

Section: Discussionmentioning

confidence: 99%

Identification of QTLs Controlling Salt Tolerance in Cucumber (Cucumis sativus L.) Seedlings

Liu

Dong

et al. 2021

Plants

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Cucumber is very sensitive to salt stress, and excessive salt content in soils seriously affects normal growth and development, posing a serious threat to commercial production. In this study, the recombinant inbred line (RIL) population (from a cross between the salt tolerant line CG104 and salt sensitive line CG37) was used to study the genetic mechanism of salt tolerance in cucumber seedlings. At the same time, the candidate genes within the mapping region were cloned and analyzed. The results showed that salt tolerance in cucumber seedlings is a quantitative trait controlled by multiple genes. In experiments carried out in April and July 2019, qST6.2 on chromosome six was repeatedly detected. It was delimited into a 1397.1 kb region, and nine genes related to salt tolerance were identified. Among these genes, Csa6G487740 and Csa6G489940 showed variations in amino acid sequence between lines CG104 and CG37. Subsequent qRT-PCR showed that the relative expression levels of both genes during salt treatment were significantly different between the two parents. These results provide a basis for the fine mapping of salt tolerant genes and further study of the molecular mechanism of salt tolerance in cucumber seedlings.

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“…Total DNA was extracted from the leaves of maize inbred line seedlings (e.g., B73, Jing724, D9H, Ky21, CML333, and Mo17) using a published CTAB method (Luo et al, 2019). Primers for amplifying ZmCd1 ( Table S4 ) were designed based on the B73 genome sequence in the MaizeGDB database (https://www.maizegdb.org/).…”

Section: Methodsmentioning

confidence: 99%

Natural variations in the P-type ATPase heavy metal transporter ZmCd1 controlling cadmium accumulation in maize grains

Tang

Luo

Zhang

et al. 2021

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SummaryCadmium (Cd) accumulation in maize grains is detrimental to human health. Developing maize varieties with low-Cd contents via marker-assisted selection is important for ensuring the production of maize grains safe for consumption. However, the key gene controlling maize grain Cd accumulation has not been cloned. In this study, we identified two major loci for maize grain Cd accumulation (qCd1 and qCd2) on chromosome 2 during a genome-wide association study (GWAS). The qCd1 locus was analyzed by bulked segregant RNA-seq and fine mapping with a biparental segregating population of Jing724 (low-Cd line) and Mo17 (high-Cd line). The ZmCd1 candidate gene in the qCd1 locus encodes a vacuolar membrane-localized heavy metal P-type ATPase transporter, ZmHMA3, which is orthologous to the tonoplast Cd transporter OsHMA3. Genomic DNA sequence and transcript analyses suggested that a transposon in intron 1 of ZmCd1 is responsible for the abnormal amino acid sequence in Mo17. An EMS mutant analysis and an allelism test confirmed ZmCd1 influences maize grain Cd accumulation. The natural variations in ZmCd1 were used to develop four PCR-based molecular markers, which revealed five ZmCd1 haplotypes in the GWAS population. The molecular markers were also used to predict the grain Cd contents in commonly cultivated maize germplasms in China. The predicted Cd contents for 36 inbred lines and 13 hybrids were consistent with the measured Cd contents. Furthermore, several low-Cd elite inbred lines and hybrids were identified, including Jing2416, MC01, Jingnonke728, and Jingke968. Therefore, the molecular markers developed in this study are applicable for molecular breeding and developing maize varieties with low grain Cd contents.

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Mapping of quantitative trait loci for seedling salt tolerance in maize

Cited by 40 publications

References 33 publications

Genome-wide identification, characterization, and expression analysis of the monovalent cation-proton antiporter superfamily in maize, and functional analysis of its role in salt tolerance

Genome-wide identification, characterization, and expression analysis of the monovalent cation-proton antiporter superfamily in maize, and functional analysis of its role in salt tolerance

Identification of QTLs Controlling Salt Tolerance in Cucumber (Cucumis sativus L.) Seedlings

Natural variations in the P-type ATPase heavy metal transporter ZmCd1 controlling cadmium accumulation in maize grains

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