Mapping of quantitative trait loci associated with resistance to net form net blotch (Pyrenophora teres f. teres) in a doubled haploid Norwegian barley population

Abstract

Barley net blotch caused by the necrotrophic fungus Pyrenophora teres is a major barley disease in Norway. It can cause grain shriveling and yield losses, and resistance in currently grown cultivars is insufficient. In this study, a set of 589 polymorphic SNP markers was used to map resistance loci in a population of 109 doubled haploid lines from a cross between the closely related Norwegian cultivars Arve (moderately susceptible) and Lavrans (moderately resistant). Resistance to three net form net blotch (P. teres f. teres) single spore isolates was evaluated at the seedling stage in the greenhouse and at the adult plant stage under field conditions during three years. Days to heading and plant height were scored to assess their influence on disease severity. At the seedling stage, three to four quantitative trait loci (QTL) associated with resistance were found per isolate used. A major, putatively novel QTL was identified on chromosome 5H, accounting for 23–48% of the genetic variation. Additional QTL explaining between 12 and 16.5% were found on chromosomes 4H, 5H, 6H and 7H, with the one on 6H being race-specific. The major QTL on 5H was also found in adult plants under field conditions in three years (explaining up to 55%) and the 7H QTL was found in field trials in one year. Additional adult plant resistance QTL on 3H, 6H and 7H were significant in single years. The resistance on chromosomes 3H, 5H, 6H and 7H originates from the more resistant parent Lavrans, while the resistance on 4H is conferred by Arve. The genetic markers associated with the QTL found in this study will benefit marker-assisted selection for resistance against net blotch.

SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres

“…The locus associated with resistance to the S10.2 isolate included one suggestive SNP (JHI-Hv50k-2016-74407) mapped in the interval 23.3-23.8 cM. We suggest that seedling resistance to the S10.2 isolate is conferred by a previously known locus mapped earlier by Wonneberger et al (2017) [7] and Vatter et al (2017) [18] (Table 5). The locus on chromosome 2H revealed for the P3.4.0 isolate included 7 SNPs and was expanded between JHI-Hv50k-2016-104508 and JHI-Hv50k-2016-104859 markers located in the interval 71.0-74.1 cM.…”

“…First, this locus was found by Steffenson et al (1996) [3], then confirmed by Manninen et al (2006) [27] and named RPt5. Wonneberger et al (2017) [7] and Afanasenko et al (2014) [2] revealed the genomic region associated with NFNB resistance in the same interval. Further loci were revealed close to this region [5,16] ( Table 5).…”

“…The genomic regions associated with resistance of barley to P. teres f. teres have been found on all barley chromosomes [3,[5][6][7][8][9][10][11][12][13][14][15][16][17][18] using both linkage mapping in biparental mapping populations and association mapping (AM). Some QTLs provide resistance during whole ontogenesis, such as QRpt6 on chromosome 6H, determining both seedling and adult resistance to the net form of net blotch [5].…”

Research advances in the Pyrenophora teres–barley interaction

“…k) and Spt1.k (previously rpt5.r ) from CI5791/CI9819, Kombar and Rika, respectively (Abu Qamar et al , ; Koladia et al , ; Manninen et al , , ; Richards et al , ) (Table ). Additional loci that may also be part of the Rpt5 locus and therefore contain additional alleles include QRpts6L , QRpt6 , AL_QRptt6‐1, Rpt‐Nomini and Rpt‐CIho2291 in Halcyon (Raman et al , ), TR251 (Grewal et al , ), Lavrans (Wonneberger et al , ), Nomini and CIho2291 (O’Boyle et al , ), respectively (Table ), as well as undesignated QTLs from Steptoe (Steffenson et al , ), HOR 9088 (Richter et al , ), Kaputar, ND11213 (Cakir et al , ; Emebiri et al , ; Liu et al , ), Chevron (Ma et al , ), SM89010 (Friesen et al , ), M129 (St. Pierre et al , ), Baudin (Cakir et al , ), WPG8412, Pompadour, Stirling (Gupta et al , ), Falcon (Islamovic et al , ), H602 (Hisano et al , ) and UVC8 (Martin et al , ) (Table ).…”

Analysis of polymorphism of microsatellite markers linked to a longterm net form of net blotch resistance gene in winter barley varieties in the south of Russia

“…In same study they found that 4H and 5H chromosomes also carry QTLs that confer resistance against net form of net blotch but in another DH population. Similarly, Wonneberger et al (2017) using a set of 589 polymorphic SNP markers mapped resistance loci in a population of 109 doubled haploid lines from a cross between Norwegian cultivars Arve and Lavrans. Three to four quantitative trait loci (QTL) associated with resistance to net blotch in the seedling stage were found per isolate used.…”