Mapping of the functional domains of the ? 4 protein of herpes simplex virus 1

Abstract

Truncated alpha 4 genes were introduced into BHK tk- cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G418, driven by the HSV-1 beta tk promoter (beta tk- neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated alpha 4 genes to regulate the expression of the beta tk- neor, and for the ability of the truncated alpha 4 polypeptides to localize to the nuclei of transformed cells. The results indicated that the domain(s) for beta gene induction and for nuclear localization of the alpha 4 protein are located within the N-terminal 288 amino acids of the protein.