Mapping of the human and mouse bone sialoprotein and osteopontin loci

Crosby, Andrew H.; Lyu, Myung Soo; Kang, Lin; McBride, O W; Kerr, Janet M.; Aplin, Helen; Fisher, Larry W.; Young, Marian F.; Kozak, Christine A.; Dixon, Michael J.

doi:10.1007/s003359900037

Cited by 33 publications

(20 citation statements)

References 21 publications

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“…This family includes osteopontin (OPN) and dentin matrix phosphoprotein (DMP1), both of which are derived from a common ancestor that is shared with other calcium-binding proteins found in enamel, milk and saliva [11]. The members of the SIBLING family are located in a syntenic gene locus that has been named the "bone gene cluster" located on murine chromosome 5 [12]. BSP shares common features with other members of this group, which are intrinsically disordered proteins with little or no secondary or tertiary structure [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Holm

Aubin

Hunter

et al. 2015

Bone

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Section: Introductionmentioning

confidence: 99%

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Holm

Aubin

Hunter

et al. 2015

Bone

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“…However, extensive conservation of genome organization exists between mammalian genomes, so the selection of positional candidates can be supplemented by genes predicted from comparative genome mapping information to map to the region of interest [14]. For example, porcine chromosome 8 (SSC8) is homologous to much of human chromosome 4 [15,16], regions of sheep chromosome 6 [17], and regions of mouse chromosome 5 [18].…”

Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Affecting Female Reproductive Traits on Porcine Chromosome 81

King

Jiang

Gibson

et al. 2003

Biology of Reproduction

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An understanding of the genetic control of porcine female reproductive performance would offer the opportunity to utilize natural variation and improve selective breeding programs through marker-assisted selection. The Chinese Meishan is one of the most prolific pig breeds known, farrowing three to five more viable piglets per litter than the European Large White breed. This difference in prolificacy is attributed to the Meishan's superior prenatal survival levels. The present study utilized a three-generation cross in which the founder grandparental animals were purebred Meishan and Large White pigs in a scan for quantitative trait loci (QTL) on porcine chromosome 8 (SSC8) associated with reproductive performance. Reproductive traits, including number of corpora lutea (ovulation rate), teat number, litter size, and prenatal survival, were recorded for as many as 220 F2 females. Putative QTL for the related traits of litter size and prenatal survival were identified at the distal end of the long arm of SSC8. A physiological candidate gene, SPP1, was found to lie within the 95% confidence interval of these QTL. A suggestive QTL for teat number was revealed on the short arm of SSC8. The present study demonstrates, to our knowledge, the first independent confirmation of QTL for fecundity on SSC8, and these QTL regions provide a crucial starting point in the search for the causal genetic variants.

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“…DNAs from the progeny of these crosses have been typed for Ͼ1000 markers distributed over the mouse genome including the chromosome 5 markers Afp (␣-fetoprotein), Gro1 (GRO1 onogene), Ibsp (bone sialoprotein), Spp1 (secreted phosphoprotein 1, also known as osteopontin) and Pdeb (phosphodiesterase ␤) which were typed as described previously (26). D5Mit9 (Chr 5 DNA marker, MIT-9) was typed using the primers and conditions described by Dietrich et al (27).…”

Section: Methodsmentioning

confidence: 99%

Genomic Organization, Chromosomal Mapping, and Promoter Analysis of the Mouse Dentin Sialophosphoprotein (Dspp) Gene, Which Codes for Both Dentin Sialoprotein and Dentin Phosphoprotein

Feng¹,

Luan²,

Wallace³

et al. 1998

Journal of Biological Chemistry

200

150

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Our laboratory has reported that two major noncollagenous dentin proteins, dentin sialoprotein and dentin phosphoprotein, are specific cleavage products of a larger precursor protein termed dentin sialophosphoprotein (MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J. Q., and Gu, T. T. (1997) J. Biol. Chem. 272:835-842). To confirm our single gene hypothesis and initiate in vitro promoter studies, we have characterized the structural organization of the mouse dentin sialophosphoprotein gene. This gene has a transcription unit of ϳ9.4 kilobase pairs and is organized into 5 exons and 4 introns. Exon 1 contains a noncoding 5 sequence, and exon 2 contains the transcriptional start site, signal peptide, and first two amino acids of the NH 2 terminus. Exons 3 and 4 contain coding information for 29 and 314 amino acids, respectively. The remainder of the coding information and the untranslated 3 region are contained in exon 5. Chromosomal mapping localized the gene to mouse chromosome 5q21 in close proximity to other dentin/bone matrix genes. Computer analysis of the promoter proximal 1.6-kilobase pair sequence revealed a number of potentially important cis-regulatory sequences; these include the recognition elements of AP-1, AP-2, Msx-1, serum response elements, SP-1, and TCF-1. In vitro studies showed that the DSPP promoter is active in an odontoblast cell line, MO6-G3, with basal activity mapped to ؊95 bp. Two potential enhancer and suppresser elements were identified in the regions between ؊1447 and ؊791 bp and ؊791 and ؊95 bp, respectively. The structural organization of the dentin sialophosphoprotein gene confirms our finding that both dentin sialoprotein and dentin phosphoprotein are encoded by a single gene with a continuous open reading frame.The dentin extracellular matrix (DECM) 1 is the result of the cytodifferentiation of cranial neural crest-derived ectomesenchymal cells that line the tooth pulp chamber into highly specialized cells termed odontoblasts. These odontoblasts express specific genes products which form the collagenous DECM. This matrix consists of mostly type I (ϳ86%), type I trimer, type III, type V, and type VI collagens and several noncollagenous proteins also found in bone extracellular matrix, such as osteonectin (OSN, also known as SPARC), osteocalcin, osteopontin (OPN, also known as SSP1), bone sialoprotein, and dentin matrix protein 1 (Dmp-1) (1, 2). However, two DECM proteins, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP, also known as phosphophoryn), have been shown to be tooth specific (for review Ref. 1), being expressed by odontoblasts and transiently by ameloblasts (2-10). These noncollagenous DECM proteins are believed to be essential for initiation and control of mineralization in the transition of predentin to dentin.DSP was first identified as a 95-kDa glycoprotein (11) with a high carbohydrate (30%) and sialic acid (10%) content accounting for 5-8% of the DECM proteins. This protein has an overall resemblance to other sialoproteins like bone sialopro...

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Mapping of the human and mouse bone sialoprotein and osteopontin loci

Cited by 33 publications

References 21 publications

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Mapping Quantitative Trait Loci Affecting Female Reproductive Traits on Porcine Chromosome 81

Genomic Organization, Chromosomal Mapping, and Promoter Analysis of the Mouse Dentin Sialophosphoprotein (Dspp) Gene, Which Codes for Both Dentin Sialoprotein and Dentin Phosphoprotein

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