Mapping Protein-Protein Proximity in the Purinosome

Deng, Yunkun; Gam, Jongsik; French, Jarrod B.; Zhao, Hong; An, Songon; Benkovic, Stephen J.

doi:10.1074/jbc.m112.407056

Cited by 61 publications

(95 citation statements)

References 13 publications

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“…Similarly to IMP, increased 15 N incorporation was observed for AMP and GMP in purine-depleted cells (Fig. 4, b and c).…”

Section: Downstream De Novo Biosynthesis Enzymes Adss and Impdh Co-mentioning

confidence: 85%

“…Fig. 4a shows 15 N incorporation as a function of time. Incorporation levels for the two conditions were the same after about 1.5 h. To calculate the rate, the incorporation within 1 h was fitted to a linear function.…”

Section: N]glycine Incorporation Into Imp Is Higher In Purinosome-ricmentioning

confidence: 99%

“…HeLa cells were pulsed with [ 15 N]glycine, a molecule incorporated into the purine base ring by the enzyme glycinamide ribonucleotide synthetase (GART) in step 2 of de novo purine biosynthesis. The incorporation of a heavy nitrogen into IMP molecules was measured over time and calculated using the amount of [ 15 15 N incorporation into IMP at longer starvation times. Therefore, the incorporation rate of IMP was measured for cells grown under normal condition (purine-rich medium) and cells having been starved for 168 h in purine-depleted medium.…”

Section: N]glycine Incorporation Into Imp Is Higher In Purinosome-ricmentioning

confidence: 99%

“…The orbitrap was operated in negative ion mode at maximum resolution (140,000) and scanned from m/z 85 to m/z 1000. The integrated areas of the reconstructed ion chromatograms for the monoisotopic and 15 N isotopologues for the [M-H] Ϫ molecular ion were measured to calculate the 15 N incorporation. Cloning of Plasmids-Adenylosuccinate synthase (ADSS) (Clone ID HsCD00295729) and inosine monophosphate dehydrogenase 2/IMPDH2 (Clone ID Hscd00042407) template cDNA were obtained from the Arizona State University Biodesign Institute plasmid repository (DNASU).…”

Section: Ultra High Pressure Liquid Chromatography Coupled With Electmentioning

confidence: 99%

“…A hypothesis was proposed that purinosomes form to satisfy the cellular demand for purines through increasing de novo purine biosynthesis (11). Additional studies surrounding purinosome composition, characterization, and regulation have been conducted (12)(13)(14)(15)(16). However, alteration of intracellular purine nucleotide pools in purinosome-containing cells, specifically whether de novo synthesis flux of the individual purines, such as IMP, AMP, and GMP, is increased, has never been reported.…”

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confidence: 99%

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Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis

Zhao

Chiaro

Zhang

et al. 2015

Journal of Biological Chemistry

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109

126

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“…Similarly to IMP, increased 15 N incorporation was observed for AMP and GMP in purine-depleted cells (Fig. 4, b and c).…”

Section: Downstream De Novo Biosynthesis Enzymes Adss and Impdh Co-mentioning

confidence: 85%

Section: N]glycine Incorporation Into Imp Is Higher In Purinosome-ricmentioning

confidence: 99%

Section: N]glycine Incorporation Into Imp Is Higher In Purinosome-ricmentioning

confidence: 99%

Section: Ultra High Pressure Liquid Chromatography Coupled With Electmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis

Zhao

Chiaro

Zhang

et al. 2015

Journal of Biological Chemistry

Self Cite

109

126

View full text Add to dashboard Cite

show abstract

The roles of molecular chaperones in regulating cell metabolism

Binder

Pedley

2023

FEBS Letters

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Fluctuations in nutrient and biomass availability, often as a result of disease, impart metabolic challenges that must be overcome in order to sustain cell survival and promote proliferation. Cells adapt to these environmental changes and stresses by adjusting their metabolic networks through a series of regulatory mechanisms. Our understanding of these rewiring events has largely been focused on those genetic transformations that alter protein expression and the biochemical mechanisms that change protein behavior, such as post‐translational modifications and metabolite‐based allosteric modulators. Mounting evidence suggests that a class of proteome surveillance proteins called molecular chaperones also can influence metabolic processes. Here, we summarize several ways the Hsp90 and Hsp70 chaperone families act on human metabolic enzymes and their supramolecular assemblies to change enzymatic activities and metabolite flux. We further highlight how these chaperones can assist in the translocation and degradation of metabolic enzymes. Collectively, these studies provide a new view for how metabolic processes are regulated to meet cellular demand and inspire new avenues for therapeutic intervention.

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An extracellular vesicular mutant KRAS‐associated protein complex promotes lung inflammation and tumor growth

Sriwastva

Teng

et al. 2023

J of Extracellular Vesicle

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Extracellular vesicles (EVs) contain more than 100 proteins. Whether there are EVs proteins that act as an ‘organiser’ of protein networks to generate a new or different biological effect from that identified in EV‐producing cells has never been demonstrated. Here, as a proof‐of‐concept, we demonstrate that EV‐G12D‐mutant KRAS serves as a leader that forms a protein complex and promotes lung inflammation and tumour growth via the Fn1/IL‐17A/FGF21 axis. Mechanistically, in contrast to cytosol derived G12D‐mutant KRAS complex from EVs‐producing cells, EV‐G12D‐mutant KRAS interacts with a group of extracellular vesicular factors via fibronectin‐1 (Fn1), which drives the activation of the IL‐17A/FGF21 inflammation pathway in EV recipient cells. We show that: (i), depletion of EV‐Fn1 leads to a reduction of a number of inflammatory cytokines including IL‐17A; (ii) induction of IL‐17A promotes lung inflammation, which in turn leads to IL‐17A mediated induction of FGF21 in the lung; and (iii) EV‐G12D‐mutant KRAS complex mediated lung inflammation is abrogated in IL‐17 receptor KO mice. These findings establish a new concept in EV function with potential implications for novel therapeutic interventions in EV‐mediated disease processes.

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Mapping Protein-Protein Proximity in the Purinosome

Cited by 61 publications

References 13 publications

Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis

Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis

The roles of molecular chaperones in regulating cell metabolism

An extracellular vesicular mutant KRAS‐associated protein complex promotes lung inflammation and tumor growth

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