Mapping QTLs for kernel oil content in a tropical maize population

QTL mapping to anthracnose leaf blight resistance in tropical maize

“…Results allowed assigning chromosomic reference to the ten maize linkage clusters by the presence of at least one SSR locus per chromosome (Figure 3). The range of the length of the linkage clusters (107.5 to 244.3 cM) and the total length of the linkage map (1710.1 cM) are in agreement with other genetic maps of tropical maize (Mangolin et al 2004). The linkage map of Castiglioni et al (1999) demonstrated greater coverage of the genome, with 2057 cM, and with markers distributed every 6.6 cM.…”

QTL verification of grain protein content and its correlation with oil content by using connected RIL populations of high-oil maize

“…However, more recently, several studies have demonstrated positive correlations between grain protein and oil contents (Dudley and Lambert, 2004;Wassom et al, 2008;Zhang et al, 2008). Further research has attempted to reveal the genetic mechanisms underlying kernel composition traits by mapping QTLs using different genetic germplasms such as IHP and ILP (Goldman et al, l993;, IHO and ILO (Alrefai et al, 1995;Berke and Rocheford, 1995;Laurie et al, 2004;Willmot et al, 2006;Wassom et al, 2008), Beinongda high-oil (BHO) (Song et al, 2004;Zhang et al, 2008), tropical high-oil (Mangolin et al, 2004), Alexho Single-Kernel (ASK) high-oil (Zheng et al, 2008;Li et al, 2009;Wang et al, 2010;Yang et al, 2012), and popcorn (Liu et al, 2008) maize populations. Based on data obtained from these studies and from the public genetic linkage map of maize (www.maizegdb.org), 79 QTLs for grain protein content and 52 QTLs for grain oil content have been detected.…”

Microsatellite-dense genetic map: towards genome coverage in a tropical maize (Zea mays L.) population

“…Genetic materials -The plant material used in this study has already been described in detail (Mangolin et al 2004). Briefl y, the parental inbred lines L-20-01F and L-02-03D were derived from the Brazilian early-fl owering maize populations IG-1 (orange fl int kernels) and IG-2 (yellow dent kernels), respectively.…”

“…Microsatellite primer selection -In order to fi ll the regions still not covered in the map developed by Mangolin et al (2004) that used the same mapping population, 392 microsatellite primers were choosen and synthesized by Invitrogen™. From those, 72 microsatellites were polymorphic based on the existing allelic polymorphism between the parental and the segregating population and these were selected for the work.…”

“…Ribault et al (1996) developed a RFLP genetic map for QTL mapping and used a crossing from two inbred lines derived from Mexican races of maize for the lowland tropics (Tuxpeño-based germplasm). Sibov et al (2003) and Mangolin et al (2004) constructed microsatellite-genetic maps in tropical maize populations derived from Brazilian and Mexican germplasm, however both of the genetic maps presented some genomic regions with large distances between markers that might be reducing the number of QTL mapped. Currently, no molecular dense genetic map has been published for tropical maize population.…”