Mapping quantitative trait loci associated with soybean resistance to common cutworm and soybean compensatory growth after defoliation using SNP marker-based genome-wide association analysis

Wang, Hui; Yan, Honglang; Du, Haiping; Chao, Maoni; Gao, Zhongjie; Yu, Deyue

doi:10.1007/s11032-015-0360-z

Cited by 11 publications

(10 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few GWASs of soybean insects or other pests such as nematodes have been carried out, and these studies have generally found few SNPs associated with resistance (Wang et al, 2015; Vuong et al, 2015; Liu et al, 2016; Chang and Hartman 2017). Because of the number of significant SNPs found in this study for each biotype, there appears to be significant genetic variation associated with soybean aphid resistance.…”

Section: Discussionmentioning

confidence: 99%

Genome‐Wide Association Mapping of Host‐Plant Resistance to Soybean Aphid

Hanson

Lorenz

Hesler³

et al. 2018

The Plant Genome

View full text Add to dashboard Cite

Core Ideas Soybean aphid host‐plant resistance is needed to reduce damage from soybean aphid. Genome‐wide association mapping is effective for finding insect resistance loci. We found single nucleotide polymorphisms associated with soybean aphid resistance for multiple aphid biotypes. Soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] is the most damaging insect pest of soybean [Glycine max (L.) Merr.] in the Upper Midwest of the United States and is primarily controlled by insecticides. Soybean aphid resistance (i.e., Rag genes) has been documented in some soybean accessions but more sources of resistance are needed. Incorporation of the resistance into marketed varieties has also been slow. Genome‐wide association mapping can aid in identifying resistant accessions by correlating phenotypic data with single nucleotide polymorphisms (SNPs) across a genome. Aphid population measures from 2366 soybean accessions were collected from published studies screening cultivated soybean (G. max) and wild soybean (Glycine soja Siebold & Zucc.) with aphids exhibiting Biotype 1, 2, or 3 characteristics. Genotypic data were obtained from the SoySNP50K high‐density genotyping array previously used to genotype the USDA Soybean Germplasm Collection. Significant associations between SNPs and soybean aphid counts were found on 18 of the 20 soybean chromosomes. Significant SNPs were found on chromosomes 7, 8, 13, and 16 with known Rag genes. SNPs were also significant on chromosomes 1, 2, 4 to 6, 9 to 12, 14, and 17 to 20 where Rag genes have not yet been mapped, suggesting that many Rag genes remain to be discovered. These SNPs can be used to determine accessions that are likely to have novel aphid resistance traits of value for breeding programs.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome‐Wide Association Mapping of Host‐Plant Resistance to Soybean Aphid

Hanson

Lorenz

Hesler³

et al. 2018

The Plant Genome

View full text Add to dashboard Cite

show abstract

“…Soybean resistance mapping using GWAS has been demonstrated on a variety of diseases (Wen et al, 2014 ; Vuong et al, 2015 ; Chang et al, 2016c ) and the resistance mechanisms have been detailed for many important soybean diseases (Whitham et al, 2016 ). However, there are just a few GWAS focusing on soybean insect resistance like that of the common cutworm (CCW, Spodoptera litura Fabricius) (Wang et al, 2015 ; Liu et al, 2016 ). With the availability of GRIN phenotypic data and SoySNP50K genotypic data, the aim of our study was to integrate the information for discovering novel insect resistance sources in the USDA Soybean Germplasm Collection and highlight candidate resistance genes for each insect.…”

Section: Discussionmentioning

confidence: 99%

“…The power of GWAS has been well recognized in numerous studies, including those on soybean agronomic traits (Zhou et al, 2015 ) and on soybean disease resistance (Chang et al, 2016a , b , c ). There are fewer GWAS focusing on insect resistance in soybean (Wang et al, 2015 ; Liu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Insect Resistance Loci in the USDA Soybean Germplasm Collection Using Genome-Wide Association Studies

Chang

Hartman

2017

Front. Plant Sci.

View full text Add to dashboard Cite

Management of insects that cause economic damage to yields of soybean mainly rely on insecticide applications. Sources of resistance in soybean plant introductions (PIs) to different insect pests have been reported, and some of these sources, like for the soybean aphid (SBA), have been used to develop resistant soybean cultivars. With the availability of SoySNP50K and the statistical power of genome-wide association studies, we integrated phenotypic data for beet armyworm, Mexican bean beetle (MBB), potato leafhopper (PLH), SBA, soybean looper (SBL), velvetbean caterpillar (VBC), and chewing damage caused by unspecified insects for a comprehensive understanding of insect resistance in the United States Department of Agriculture Soybean Germplasm Collection. We identified significant single nucleotide (SNP) polymorphic markers for MBB, PLH, SBL, and VBC, and we highlighted several leucine-rich repeat-containing genes and myeloblastosis transcription factors within the high linkage disequilibrium region surrounding significant SNP markers. Specifically for soybean resistance to PLH, we found the PLH locus is close but distinct to a locus for soybean pubescence density on chromosome 12. The results provide genetic support that pubescence density may not directly link to PLH resistance. This study offers a novel insight of soybean resistance to four insect pests and reviews resistance mapping studies for major soybean insects.

show abstract

“… Ghione et al (2021) found four markers associated with resistance genes against stink bugs using the association mapping strategy. To date, several insect-resistance QTLs, specific for leaf-chewing insects, have been identified in the soybean germplasm ( Rector et al, 2000 ; Zhu et al, 2008 ; Wang et al, 2015 ; Ortega et al, 2016 ; Sabljic et al, 2020 ; Ghione et al, 2021 ; Lucini et al, 2021 ). Interestingly, Ortega et al (2017) reported that the Glyma.07g110300 marker (encoding a UDP-glycosyltransferase) in QTL-M was a functional single-nucleotide polymorphism (SNP) that could be used for the marker-assisted selection of insect-resistance QTLs and Glyma.07g110300 exhibits a loss of function in insect-resistant soybean germplasms.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Targeted Mutagenesis of GmUGT Enhanced Soybean Resistance Against Leaf-Chewing Insects Through Flavonoids Biosynthesis

et al. 2022

View full text Add to dashboard Cite

Leaf-chewing insects are important pests that cause yield loss and reduce seed quality in soybeans (Glycine max). Breeding soybean varieties that are resistant to leaf-chewing insects can minimize the need for insecticide use and reduce yield loss. The marker gene for QTL-M, Glyma.07g110300 (LOC100775351) that encodes a UDP-glycosyltransferase (UGT) is the major determinant of resistance against leaf-chewing insects in soybean; it exhibits a loss of function in insect-resistant soybean germplasms. In this study, Agrobacterium-mediated transformation introduced the CRISPR/Cas9 expression vector into the soybean cultivar Tianlong No. 1 to generate Glyma.07g110300-gene mutants. We obtained two novel types of mutations, a 33-bp deletion and a single-bp insertion in the GmUGT coding region, which resulted in an enhanced resistance to Helicoverpa armigera and Spodoptera litura. Additionally, overexpressing GmUGT produced soybean varieties that were more sensitive to H. armigera and S. litura. Both mutant and overexpressing lines exhibited no obvious phenotypic changes. The difference in metabolites and gene expression suggested that GmUGT is involved in imparting resistance to leaf-chewing insects by altering the flavonoid content and expression patterns of genes related to flavonoid biosynthesis and defense. Furthermore, ectopic expression of the GmUGT gene in the ugt72b1 mutant of Arabidopsis substantially rescued the phenotype of H. armigera resistance in the atugt72b1 mutant. Our study presents a strategy for increasing resistance against leaf-chewing insects in soybean through CRISPR/Cas9-mediated targeted mutagenesis of the UGT genes.

show abstract

Mapping quantitative trait loci associated with soybean resistance to common cutworm and soybean compensatory growth after defoliation using SNP marker-based genome-wide association analysis

Cited by 11 publications

References 62 publications

Genome‐Wide Association Mapping of Host‐Plant Resistance to Soybean Aphid

Genome‐Wide Association Mapping of Host‐Plant Resistance to Soybean Aphid

Characterization of Insect Resistance Loci in the USDA Soybean Germplasm Collection Using Genome-Wide Association Studies

CRISPR/Cas9-Mediated Targeted Mutagenesis of GmUGT Enhanced Soybean Resistance Against Leaf-Chewing Insects Through Flavonoids Biosynthesis

Contact Info

Product

Resources

About