Mapping Seed Phytic Acid Concentration and Iron Bioavailability in a Pea Recombinant Inbred Line Population

Abstract

Phytate, the storage form of P in seeds, is not well digested by monogastrics, thereby contributing to micronutrient deficiency, decreased feed efficiency, and environmental pollution. This research was aimed at developing a single nucleotide polymorphism (SNP) based genetic linkage map and mapping genomic regions associated with phytic acid‐phosphorus (PA‐P) concentration using a recombinant inbred line (RIL) population (PR‐15) derived from a cross between a low phytate (low phytic acid [lpa]) mutant pea (Pisum sativum L.) genotype, 1‐2347‐144, and a normal phytate cultivar CDC Meadow. A total of 163 RILs were genotyped using a 1536‐SNP Illumina GoldenGate array. Three hundred and sixty‐seven polymorphic SNP markers ordered into seven linkage groups (LGs) were used to generate a linkage map with a total length of 437.2 cM. PR‐15 lines were grown in replicated field trails in Saskatoon and Rosthern, SK, in 2012 and 2013. Chi‐square statistics confirmed the single gene inheritance of PA‐P concentration in these RILs. Phytic acid‐phosphorus (PA‐P) phenotype was mapped to LG5. Iron bioavailability (FEBIO) of PR‐15 lines estimated using the Caco‐2 cell culture bioassay was negatively correlated with PA‐P concentration. A quantitative trait locus (QTL) for FEBIO was mapped on to the same location on LG5 as phytic acid concentration. The QTL with a maximum LOD score of 15.1 explained 60.5% of the phenotypic variation in FEBIO. The markers flanking this QTL region can be employed in marker‐assisted selection to select pea lines with low phytate and greater Fe bioavailability.

Construction of high-density linkage maps for mapping quantitative trait loci for multiple traits in field pea (Pisum sativum L.)

“…Ma et al [ 36 ] reported that the QTL with highest explanation of phenotypic variance for seed zinc concentration in a mapping population derived from Aragorn x Kiflica tested in two different locations was also located on LG3. Shunmugam et al [ 50 ] also reported a major QTL for seed phytate concentration in pea on LG5, and this QTL was confirmed here using an expanded set of markers.…”

Genetic dissection of seed-iron and zinc concentrations in chickpea

“…Tremendous efforts have been made to delve deeper into the complex genetic architecture of this trait in several crop species, including cereals and legumes 2 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 . Among legumes with much emphasis on common bean, attempts have been made to decipher the underlying genetic factors regulating Fe and Zn concentrations in seeds of Medicago , lentil, field pea and chickpea 8 9 13 14 20 21 22 23 24 .…”

Iron Bioavailability in Field Pea Seeds: Correlations with Iron, Phytate, and Carotenoids

“…The concentration of phytate P was reduced by ∼60% in 1‐2347‐144 and 1‐150‐81 compared with CDC Bronco. Along with low phytate acid P (PA‐P) concentration, there was a commensurate increase in inorganic P (P i ) (Shunmugam et al, 2015) The two low‐phytate lines, 1‐2347‐144 and 1‐150‐81, produced grain yield 92 and 86% of CDC Bronco, respectively (Delgerjav, 2012). From a nutritional standpoint, FeBIO was up to 1.9 times higher in the two low‐phytate lines than CDC Bronco (Liu et al, 2014).…”