Mapping Social Behavior-Induced Brain Activation at Cellular Resolution in the Mouse

Kim, Yongsoo; Venkataraju, Kannan Umadevi; Pradhan, Kith; Mende, Carolin; Taranda, Julián; Turaga, Srinivas C.; Arganda‐Carreras, Ignacio; Ng, Lydia; Hawrylycz, Michael; Rockland, Kathleen S.; Seung, H. Sebastian; Osten, Pavel

doi:10.1016/j.celrep.2014.12.014

Cited by 303 publications

(317 citation statements)

References 59 publications

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“…Serial two-photon tomography is a method forobtaining fluorescent images in an automated manner with the capacity to capture 260 50 mM serial sections throughout an entire mouse brain at 10Â resolution within several hours (Ragan et al 2012). This imaging approached was applied to cfos-GFP transgenic mice and combined with an analysis pipeline for automated cell counting and anatomical partitioning to obtain whole brain activity maps at cellular resolution in social behavior (Kim et al 2015). The results were generally consistent with previous studies showing activation in appropriate areas of the hypothalamus and amygdalawith social interaction and served as a proof of principle for automated whole brain imaging of activity using an IEG-based reporter.…”

Section: Ieg-based Transgenicssupporting

confidence: 71%

“…Finally, IEG-linked GFP reporters have been used to detect neural activity on a brain-wide scale (Kim et al 2015;Vousden et al 2015). Serial two-photon tomography is a method forobtaining fluorescent images in an automated manner with the capacity to capture 260 50 mM serial sections throughout an entire mouse brain at 10Â resolution within several hours (Ragan et al 2012).…”

Section: Ieg-based Transgenicsmentioning

confidence: 99%

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Exploring Memory Representations with Activity-Based Genetics

Mayford

Reijmers

2015

Cold Spring Harb Perspect Biol

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The brain is thought to represent specific memories through the activity of sparse and distributed neural ensembles. In this review, we examine the use of immediate early genes (IEGs), genes that are induced by neural activity, to specifically identify and genetically modify neurons activated naturally by environmental experience. Recent studies using this approach have identified cellular and molecular changes specific to neurons activated during learning relative to their inactive neighbors. By using opto-and chemogenetic regulators of neural activity, the neurons naturally recruited during learning can be artificially reactivated to directly test their role in coding external information. In contextual fear conditioning, artificial reactivation of learning-induced neural ensembles in the hippocampus or neocortex can substitute for the context itself. That is, artificial stimulation of these neurons can apparently cause the animals to "think" they are in the context. This represents a powerful approach to testing the principles by which the brain codes for the external world and how these circuits are modified with learning.

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Section: Ieg-based Transgenicssupporting

confidence: 71%

Section: Ieg-based Transgenicsmentioning

confidence: 99%

Exploring Memory Representations with Activity-Based Genetics

Mayford

Reijmers

2015

Cold Spring Harb Perspect Biol

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“…Ahrens et al, 2013) or have low temporal resolution (e.g. Kim et al, 2015). Advances that allow large-scale neuronal recordings while animals are performing unconstrained social behaviors will provide a more finely grained and mechanistic understanding of the neural circuits underlying social behavior.…”

Section: Discussionmentioning

confidence: 99%

Machine vision methods for analyzing social interactions

Robie

Seagraves

Egnor

et al. 2017

Journal of Experimental Biology

126

101

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Recent developments in machine vision methods for automatic, quantitative analysis of social behavior have immensely improved both the scale and level of resolution with which we can dissect interactions between members of the same species. In this paper, we review these methods, with a particular focus on how biologists can apply them to their own work. We discuss several components of machine vision-based analyses: methods to record high-quality video for automated analyses, video-based tracking algorithms for estimating the positions of interacting animals, and machine learning methods for recognizing patterns of interactions. These methods are extremely general in their applicability, and we review a subset of successful applications of them to biological questions in several model systems with very different types of social behaviors.

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“…Similarly, the MEMRI signal measured one week after CSDS could reflect neuronal activation related to long-term physiological and behavioral changes observed in defeated animals 10 . The regions we selected for immunohistochemical analysis have been implicated in the processing of social and emotional behaviour in mice and humans 6,23,24,28 . However, we only observed repeated activation of a subset of these regions during chronic psychosocial stress in mice.…”

Section: Discussionmentioning

confidence: 99%

Brain activation induced by chronic psychosocial stress in mice

Laine

Sokołowska

Dudek

et al. 2017

European Neuropsychopharmacology

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Chronic psychosocial stress is a well-established risk factor for neuropsychiatric diseases. Abnormalities in brain activity have been demonstrated in patients with stress-related disorders. Global brain activation patterns during chronic stress exposure are less well understood but may have strong modifying effects on specific brain circuits and thereby influence development of stress-related pathologies. We determined neural activation induced by chronic social defeat stress, a mouse model of psychosocial stress. To assess chronic activation with an unbiased brain-wide focus we used manganese-enhanced magnetic resonance imaging (MEMRI) and immunohistochemical staining of ∆FOSB, a transcription factor induced by repeated neural activity. One week after 10-day social defeat we observed significantly more activation in several brain regions known to regulate depressive and anxiety-like behaviour, including the prefrontal cortex, bed nucleus of stria terminalis, ventral hippocampus and periaqueductal grey in stressed compared to control mice. We further established that the correlation of ∆FOSB positive cells between specific brain regions was altered following chronic social defeat. Chronic activation of these neural circuits may relate to persistent brain activity changes occurring during chronic psychosocial stress exposure, with potential relevance for the development of anxiety and depression in humans.Psychosocial stress increases the risk for both somatic and psychiatric diseases and worsens their prognosis 1-4 . This term refers to a broad range of experiences, including lack of normative care, abuse, and problems in peer relationships such as bullying 5 . Stress-related mental disorders like major depressive disorder (MDD) and anxiety disorders are associated with altered activation of specific brain regions. For example, neuroimaging has revealed that resting-state activity is reduced in the prefrontal cortex (PFC) 6 and increased in the hippocampus 7 while task-related reactivity is decreased in the hippocampus 8 and nucleus accumbens 9 of MDD patients. Interpretation of the human imaging findings is difficult as activation patterns in tasks carried out during brain imaging may differ from those occurring during naturalistic chronic stress exposure. Because patients enrolled in these studies also tend to have an extensive history of symptoms and medication use, it is challenging to reveal direct consequences of chronic psychosocial stress itself. In contrast, animal models allow investigation of specific stressors in a controlled manner to establish the mechanisms connecting chronic psychosocial stress exposure, brain activation, and subsequent emotional and social behaviour.Chronic social defeat stress (CSDS) is a rodent model of psychosocial stress, which produces many behavioural features similar to the symptoms of human anxiety and mood disorders, such as social aversion, anhedonia (decreased sucrose-preference) and increased self-administration of drugs of abuse 10,11 . These behavioural phenotypes...

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Mapping Social Behavior-Induced Brain Activation at Cellular Resolution in the Mouse

Cited by 303 publications

References 59 publications

Exploring Memory Representations with Activity-Based Genetics

Exploring Memory Representations with Activity-Based Genetics

Machine vision methods for analyzing social interactions

Brain activation induced by chronic psychosocial stress in mice

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