Mapping spot blotch resistance genes in four barley populations

Bovill, Jessica; Lehmensiek, A.; Sutherland, Mark W.; Platz, G. J.; Usher, T.; Franckowiak, J. D.; Mace, Emma

doi:10.1007/s11032-010-9401-9

Cited by 28 publications

(18 citation statements)

References 25 publications

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“…The CRR QTL identified in the present study were not located in regions previously associated with spot blotch resistance (Bovill et al 2010), suggesting that genetic control of CRR resistance and spot blotch resistance are independently inherited. This is in agreement with previous findings (Kutcher et al 1996a).…”

Section: Discussioncontrasting

confidence: 59%

“…However, before that is possible, robust PCR-based markers for use in routine screening will need to be developed from sequence information for the relevant DArT probes (Bovill et al 2010). …”

Section: Discussionmentioning

confidence: 99%

“…Primer sequences for SSR markers which mapped near putative QTL on a consensus map (Wenzl et al 2006) were obtained from GrainGenes (http://wheat.pw.usda.gov/GG2/index.shtml) and a standard protocol was used to amplify the markers (Bovill et al 2010). The amplified products were visualised using a Gel-Scan 2000™ (Corbett Life Sciences, Sydney, Australia).…”

Section: Single Sequence Repeat (Ssr) Analysismentioning

confidence: 99%

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Genomic regions associated with common root rot resistance in the barley variety Delta

Lehmensiek

Bovill

Sutherland

et al. 2010

Austral. Plant Pathol.

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Common root rot (CRR) caused by Bipolaris sorokiniana is a serious disease constraint in the dry temperate cereal growing regions of the world. Currently little is known about the genetic control of resistance to CRR in cereals. In this study based on a Delta/Lindwall barley population we have undertaken a bulked segregant analysis (BSA) and whole genome mapping approach utilising Diversity Arrays Technology (DArT) to identified quantitative trait loci (QTL) associated with CRR expression.One QTL each was identified on chromosomes 4HL and 5HL explaining 12 and 11% of the phenotypic variance, respectively. IntroductionThe fungal pathogen Bipolaris sorokiniana (teleomorph: Cochliobolus sativus (Ito & Kuribayashi) Drechs. ex Dastur), is the causal agent of the foliar disease spot blotch and the root disease common root rot (CRR) in winter cereals. Common root rot infected seedlings develop dark brown necrotic lesions on the roots, crown and lower leaf sheaths (Kumar et al. 2002). The disease impairs the functioning of roots and crown, resulting in fewer tillers and heads, which causes a reduction in grain yield and quality (Wildermuth et al. 1992). As high soil temperatures and low soil moisture appear to contribute to the severity of the disease, damage due to CRR can vary widely from year to year in a given location (Van Leur et al. 1997;Kumar et al. 2002;Mathre et al. 2003). This disease is a significant constraint in the dryer temperate cereal growing regions of the world, and has been particularly damaging in Syria, Canada, USA and Australia (Wildermuth 1986;Conner et al. 1996;Van Leur et al. 1997;Kumar et al. 2002;Tobias et al. 2009). In Australia, CRR has been reported in wheat and barley cropping fields in every mainland state, with estimated yield losses of up to 24% reported in Queensland (Wildermuth et al. 1992).Integrated strategies for controlling B. sorokiniana on barley include soil and residue management, chemical control, crop rotation and resistance breeding (Mehta 1988; Steffenson 1997). Wildermuth and McNamara (1991) demonstrated that rotational strategies involving a range of other crops, in particular lucerne, resulted in the reduction of soil populations of B. sorokiniana and thus a reduction in the severity of CRR. However, after a Phenotypic screening for CRR resistance generally relies on assessment of visual discolouration along the sub-crown internode and on the roots (Tobias et al. 2009) of advanced plant materials sampled from the field. These methods are subjective, time consuming, expensive and prone to significant environmental interactions. Due to these limitations, molecular marker technologies combined with conventional breeding methods may provide a faster and more robust means of identifying resistant progeny, thus increasing the efficiency of selection in cereal breeding programs.This study examined a population of recombinant inbred barley lines (RILs) developed from a cross between the moderately resistant parent variety Delta and the susceptible variety Lindwall, with the...

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Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Single Sequence Repeat (Ssr) Analysismentioning

confidence: 99%

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Genomic regions associated with common root rot resistance in the barley variety Delta

Lehmensiek

Bovill

Sutherland

et al. 2010

Austral. Plant Pathol.

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“…Another QTL on 7H ( HvWaxyA‐EBmac603 ) was coincident with Rcs‐qtl‐7H‐2‐4 (Bilgic et al. 2005), one QTL reported by Bovill et al. (2010), and one QTL reported by Roy et al.…”

Section: Discussionmentioning

confidence: 84%

“…The QTL on 3H (Bmag136-Bmag603) and one QTL located on 7H (Bmac156-Bmag135) were mapped in regions without previous reports of SB resistance QTLs. Another QTL on 7H (HvWaxyA-EBmac603) was coincident with Rcs-qtl-7H-2-4 (Bilgic et al 2005), one QTL reported by Bovill et al (2010), and one QTL reported by Roy et al (2010) although the latter was contributed by wild barley so the association is tentative.…”

Section: Chrmentioning

confidence: 81%

Quantitative trait locus analysis of spot blotch and leaf rust resistance in the BCD47 × Baronesse barley mapping population

et al. 2012

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We studied the genetics of the resistance to leaf rust (LR) (caused by Puccinia hordei) and spot blotch (SB) (caused by Cochliobolus sativus) in barley using a doubled-haploid population derived from the cross BCD47 · Baronesse. BCD47 has low SB severity and high susceptibility to LR, while Baronesse is susceptible to SB and has low LR severity. Resistance to both diseases is expressed at the adult plant stage. The population was phenotyped in eight field environments for SB and nine for LR. Ten quantitative trait loci (QTLs) were detected for SB. None were significant in more than three environments, and both parents contributed resistance alleles. Five QTLs were detected for LR. The most consistent quantitative trait locus (QTL) (significant in seven environments) was on chromosome 6H (located on the Bmag173-Bmag009 interval) with Baronesse contributing the resistance allele. Coincident QTL effects for SB were also detected in this region with resistance alleles to the two diseases in repulsion. These results illustrate the difficulties of resistance gene detection in the complex disease environments found under field conditions.

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Gain from genomic selection for a selection index in two‐row spring barley

2021

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New breeding programs are faced with many challenges including evaluation of unknown germplasm, initiation of breeding populations that will satisfy short-and long-term breeding goals, and implementation of efficient phenotyping strategies for multiple traits. Genomic selection (GS) is a potentially valuable tool for recently established breeding programs to quickly accelerate genetic gain. Genomic selection on selection index (SI) values may increase gain over phenotypic selection but empirical studies remain limited. We compared gain in overall SI value for height, heading date, preharvest sprouting (PHS) resistance, and spot blotch resistance and component traits in two cycles of GS with one round of phenotypic selection (PS) in two-row spring malting barley (Hordeum vulgare L.). Higher realized gain for SI value, height, and PHS was observed with GS compared with PS but GS did not result in significant gain for heading date and spot blotch. Genetic variances for height and heading date, which had small index weights, were not reduced with GS but variances were substantially reduced for heavily weighted PHS and correlated seed germination traits. Inbreeding was increased by GS compared with PS but restricted mating of high breeding value individuals limited potential inbreeding. Our results indicate GS is a useful method to improve selection on index values with different weights.

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Mapping spot blotch resistance genes in four barley populations

Cited by 28 publications

References 25 publications

Genomic regions associated with common root rot resistance in the barley variety Delta

Genomic regions associated with common root rot resistance in the barley variety Delta

Quantitative trait locus analysis of spot blotch and leaf rust resistance in the BCD47 × Baronesse barley mapping population

Gain from genomic selection for a selection index in two‐row spring barley

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