Mapping sugar beet pectin acetylation pattern

Ralet, Marie-Christine; Cabrera, Juan Carlos; Bonnin, Estelle; Quéméner, Bernard; Hellín, Pilar; Thibault, Jean-François

doi:10.1016/j.phytochem.2005.06.003

Cited by 119 publications

(72 citation statements)

References 26 publications

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“…al., 2005b) presumed to be the RG-I fraction, although it has been shown previously (Ralet, et. al., 2005a) The RG-I region was found to contain 22 mol% GalA; 17 mol% Rha and 62 mol% neutral 272 sugars (GalA: Rha: NS ratio of 1.3: 1.0: 3.6) suggesting that this fraction is indeed the RG-I 273 region and the HG component has been almost entirely degraded, this was also supported by a 274 further increase in the amount of ferulic acid to 2.4 %, which is 3 times that present in pectin A 275 and is slightly larger than the increase in total neutral sugars ~ 2.6 times ( Table 1).…”

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confidence: 80%

Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin

Morris

Ralet

Bonnin

et al. 2010

Carbohydrate Polymers

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103

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confidence: 80%

Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin

Morris

Ralet

Bonnin

et al. 2010

Carbohydrate Polymers

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103

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“…The mum2-11 full MS spectrum exhibited additional peaks that might correspond to RG oligomers carrying one or two hexose residues (Schols et al, 1995;Renard et al, 1998) (Figure 10, Table 2). Detailed linkage and branching patterns were established for these different oligomers using electrospray ion trap mass spectrometry (Qué mé ner et al, 2003a(Qué mé ner et al, , 2003bRalet et al, 2005). For unbranched RG-I oligosaccharides, fragmentation analyses conducted on the different parent ions yielded daughter ions that agreed with alternating 4-linked GalA units and 2-linked Rha units, the reducing ends being GalA units (Cancilla et al, 1998).…”

Section: Sugar Analysis Of the Soluble Outer Layer And Adherent Innermentioning

confidence: 99%

A Naturally Occurring Mutation in an Arabidopsis Accession Affects a β-d-Galactosidase That Increases the Hydrophilic Potential of Rhamnogalacturonan I in Seed Mucilage

et al. 2007

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The Arabidopsis thaliana accession Shahdara was identified as a rare naturally occurring mutant that does not liberate seed mucilage on imbibition. The defective locus was found to be allelic to the mum2-1 and mum2-2 mutants. Map-based cloning showed that MUCILAGE-MODIFIED2 (MUM2) encodes the putative b-D-galactosidase BGAL6. Activity assays demonstrated that one of four major b-D-galactosidase activities present in developing siliques is absent in mum2 mutants. No difference was observed in seed coat epidermal cell structure between wild-type and mutant seed; however, weakening of the outer tangential cell wall by chemical treatment resulted in the release of mucilage from mum2 seed coat epidermal cells, and the mum2 mucilage only increased slightly in volume, relative to the wild type. Consistent with the absence of b-D-galactosidase activity in the mutant, the inner layer of mucilage contained more Gal. The allocation of polysaccharides between the inner and outer mucilage layers was also modified in mum2. Mass spectrometry showed that rhamnogalacturonan I in mutant mucilage had more branching between rhamnose and hexose residues relative to the wild type. We conclude that the MUM2/BGAL6 b-Dgalactosidase is required for maturation of rhamnogalacturonan I in seed mucilage by the removal of galactose/galactan branches, resulting in increased swelling and extrusion of the mucilage on seed hydration.

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“…Oligogalacturonides (OGalA) were prepared through the autolysis of PGalA as previously described (32). PGalA solution (1% [wt/vol]) in deionized water was incubated at 100°C for 25 h. The solution was then precipitated with ethanol (64% [vol/vol] final concentration) at 4°C for 18 h. The precipitate and supernatant were harvested by centrifugation at 9,816 ϫ g for 15 min.…”

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Microbial Utilization and Selectivity of Pectin Fractions with Various Structures

Onumpai

Kolida

Bonnin

et al. 2011

Appl Environ Microbiol

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113

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To evaluate the fermentation properties of oligosaccharides derived from pectins and their parent polysaccharides, a 5-ml-working-volume, pH-and temperature-controlled fermentor was tested. Six pectic oligosaccharides representing specific substructures found within pectins were prepared. These consisted of oligogalacturonides (average degrees of polymerization [DP] of 5 and 9), methylated oligogalacturonides (average DP of 5), oligorhamnogalacturonides (average DP of 10 as a disaccharide unit of galacturonic acid and rhamnose), oligogalactosides (average DP of 5), and oligoarabinosides (average DP of 6). The influence of these carbohydrates on the human fecal microbiota was evaluated. Use of neutral sugar fractions resulted in an increase in Bifidobacterium populations and gave higher organic acid yields. The Bacteroides-Prevotella group significantly increased on all oligosaccharides except oligogalacturonides with an average DP of 5. The most selective substrates for bifidobacteria were arabinan, galactan, oligoarabinosides, and oligogalactosides.Pectins are complex plant heteropolysaccharides consisting in the main of four types of carbohydrate structure. These are partially methyl and/or acetyl esterified polygalacturonic acid (PGalA), rhamnogalacturonan type I (RG I), xylogalacturonan, and rhamnogalacturonan type II (RG II). PGalA is a linear homopolymer of ␣-(1,4)-galacturonic acid. Xylogalacturonan consists of a PGalA backbone with xylose bound to galactopyranosyluronic acid (GalpA) at C-3. RG I and RG II comprise the so-called "hairy regions" due to their branched structures. The -4-␣-D-GalAp-1,2-␣-L-Rhap-1-disaccharide sequence constitutes the main chain of RG I, with substitutions at position 4 of the rhamnose residues as well as acetylation on C-2 or C-3 of the galacturonic acid. Side chains are ␣-(1,5)-arabinan, ␤-(1,4)-galactan, and arabinogalactan type I. RG II is very complex, but it is believed to be the most conserved part of pectin. This molecule has PGalA as a backbone and is branched with rhamnose and other rare sugars such as apiose, 2-O-methylxylose, and 2-O-methylfucose (31).The biological effects of pectins have been studied extensively, and they are reported to be highly fermentable dietary fibers (5). Apart from their complexity and heterogeneity, both pectins and their derivatives have been investigated for many other potential health benefits. Pectins are found in oriental medicinal herbs and are thought to be responsible for their therapeutic effects (18,27). Studies to date indicate that different regions of pectins may mediate the different health effects. For example, modified citrus pectin may control the progress of malignancy (12) or induce apoptosis in cancer cell lines (20,29). In terms of the selectivity of fermentation by fecal bacteria, pectic oligosaccharides had greater selectivity toward bifidobacteria in batch cultures than the parent pectin (24,25,30).Many plant by-products are rich in pectins, which could possibly be sources of active pectic compounds and may prove ...

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Mapping sugar beet pectin acetylation pattern

Cited by 119 publications

References 26 publications

Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin

Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin

A Naturally Occurring Mutation in an Arabidopsis Accession Affects a β-d-Galactosidase That Increases the Hydrophilic Potential of Rhamnogalacturonan I in Seed Mucilage

Microbial Utilization and Selectivity of Pectin Fractions with Various Structures

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