Mapping the genetic basis for sex determination and growth in hybrid tilapia (Oreochromis mossambicus × O. niloticus)

Wang, Le; Sun, Fei; Yang, Zituo; Lee, May; Yeo, Shadame; Wong, Joey; Wen, Yanfei; Yue, Gen Hua

doi:10.1016/j.aquaculture.2024.741310

Aquaculture

2024

DOI: 10.1016/j.aquaculture.2024.741310

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Mapping the genetic basis for sex determination and growth in hybrid tilapia (Oreochromis mossambicus × O. niloticus)

Le Wang,

Fei Sun,

Zituo Yang

et al.

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Cited by 2 publications

References 77 publications

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Salt tolerance candidate genes identified by QTL mapping, RNA-seq, and functional analysis in tilapia

Yang,

Wang,

Sun

et al. 2025

Aquaculture

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Salt tolerance candidate genes identified by QTL mapping, RNA-seq, and functional analysis in tilapia

Yang,

Wang,

Sun

et al. 2025

Aquaculture

View full text Add to dashboard Cite

Molecular Characterization, Expression Analysis, and CRISPR/Cas9 Mediated Gene Disruption of Myogenic Regulatory Factor 4 (MRF4) in Nile Tilapia

Sukhan,

Cho,

Hossen

et al. 2024

CIMB

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Myogenic regulator factors (MRFs) are essential for skeletal muscle development in vertebrates, including fish. This study aimed to characterize the role of myogenic regulatory factor 4 (MRF4) in muscle development in Nile tilapia by cloning NT-MRF4 from muscle tissues. To explore the function of NT-MRF4, CRISPR/Cas9 gene editing was employed. The NT-MRF4 cDNA was 1146 bp long and had encoded 225 amino acids, featuring a myogenic basic domain, a helix-loop-helix domain, and a nuclear localization signal. NT-MRF4 mRNA was exclusively expressed in adult muscle tissues, with expression also observed during embryonic and larval stages. Food-deprived Nile tilapia exhibited significantly lower NT-MRF4 mRNA levels than the controls while re-feeding markedly increased expression. The CRISPR/Cas9 gene editing of NT-MRF4 successfully generated two types of gene disruption, leading to a frame-shift mutation in the NT-MRF4 protein. Expression analysis of MRF and MEF2 genes in gene-edited (GE) Nile tilapia revealed that MyoG expressions nearly doubled compared to wild-type (WT) fish, suggesting that MyoG compensates for the loss of MRF4 function. Additionally, MEF2b, MEF2d, and MEF2a expressions significantly increased in GE Nile tilapia, supporting continued muscle development. Overall, these findings suggest that NT-MRF4 regulates muscle development, while MyoG may compensate for its inactivation to sustain normal muscle growth.

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Mapping the genetic basis for sex determination and growth in hybrid tilapia (Oreochromis mossambicus × O. niloticus)

Cited by 2 publications

References 77 publications

Salt tolerance candidate genes identified by QTL mapping, RNA-seq, and functional analysis in tilapia

Salt tolerance candidate genes identified by QTL mapping, RNA-seq, and functional analysis in tilapia

Molecular Characterization, Expression Analysis, and CRISPR/Cas9 Mediated Gene Disruption of Myogenic Regulatory Factor 4 (MRF4) in Nile Tilapia

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