Mapping the Genomic Regions Controlling Germination Rate and Early Seedling Growth Parameters in Rice

Mohanty, S.; Nayak, D. K.; Sanghamitra, Priyadarsini; Barik, Saumya Ranjan; Pandit, Elssa; Behera, Abhisarika; Pani, Dipti Ranjan; Mohapatra, SD; R., Reshmi Raj K.; Pradhan, K.; Sahoo, Chita Ranjan; Mohanty, Mahendra Kumar; Behera, Chinmayee; Panda, Alok Kumar; Jena, Binod Kumar; Behera, Lambodar; Dash, Prasanta K.; Pradhan, Sharat Kumar

doi:10.3390/genes14040902

Cited by 2 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The interaction between all morphological traits was depicted through a biplot graph in the matrix. All the plots and results of PCA were generated as per the standard procedure followed in earlier publications [ 51 , 52 , 53 , 54 ].…”

Section: Methodsmentioning

confidence: 99%

Transfer of Stress Resilient QTLs and Panicle Traits into the Rice Variety, Reeta through Classical and Marker-Assisted Breeding Approaches

Barik

Moharana

Pandit

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Reeta is a popular late-maturing high-yielding rice variety recommended for cultivation in the eastern Indian states. The cultivar is highly sensitive to submergence stress. Phosphorus deficiency is an additional constraint for realizing high yield. The quantitative trait loci (QTLs), Sub1, for submergence and Pup1 for low phosphorus stress tolerance along with narrow-grained trait, GW5 were introgressed into the variety from the donor parent, Swarna-Sub1 through marker-assisted breeding. In addition, phenotypic selections for higher panicle weight, grain number, and spikelet fertility were performed in each segregating generation. Foreground selection detected the 3 target QTLs in 9, 8 and 7 progenies in the BC1F1, BC2F1, and BC3F1 generation, respectively. Recurrent parent’s genome recovery was analyzed using 168 SSR polymorphic markers. The foreground analysis in 452 BC3F2 progenies showed five pyramided lines in homozygous condition for the target QTLs. No donor fragment drag was noticed in the Sub1 and GW5 QTLs carrier while a segmentwas observed in the Pup1 carrier chromosome. The developed lines were higher yielding, had submergence, and had low phosphorus stress-tolerance alongwith similar to the recipient parent in the studied morpho-quality traits. A promising pyramided line is released in the name of Reeta-Panidhan (CR Dhan 413) for the flood-prone areas of Odisha state.

show abstract

Section: Methodsmentioning

confidence: 99%

Transfer of Stress Resilient QTLs and Panicle Traits into the Rice Variety, Reeta through Classical and Marker-Assisted Breeding Approaches

Barik

Moharana

Pandit

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…At each data collection point, the germinated seeds are carefully extracted from the culture dish. The calculation formulas for germination rate (GR) (Metwally et al, 2022), germination potential (GP) (Wang et al, 2023), germination index (GI) (Aloui et al, 2014;Chen et al, 2018), mean germination time (MGT) (Ullah et al, 2022;Mohanty et al, 2023), and peak germination (PG) (Arnolds et al, 2015) are provided below.…”

Section: Materials and Exogenous Hormone Treatmentmentioning

confidence: 99%

The identification of suitable internal reference genes in quinoa seeds subjected to abscisic acid and gibberellin treatment

Gao,

Pan,

Zeng

et al. 2023

J. Seed Sci.

View full text Add to dashboard Cite

Quinoa has been recognized as the sole “comprehensive nutritional crop”; however, it is susceptible to pre-harvest sprouting (PHS). While quantitative reverse transcription polymerase chain reaction (RT-qPCR) has been extensively employed for gene expression level detection, the selection of suitable reference genes is imperative to ensure precise gene expression quantification across diverse conditions. This study aims to identify stable reference genes in quinoa seeds under ABA and GA, in order to provide a basis for subsequent research on PHS. Seeds were subjected to different concentrations of ABA and GA (10 μM, 50 μM, 100 μM, and 200 μM). The most suitable treatment concentration was determined based on seed viability. Here, MON1, GAPDH, EIF3, EF1α, ACT, TUB1, and TUB6 were selected as candidate genes. The suitability of these reference genes under different conditions was assessed using various methods including Ct values, geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. Based on the results obtained from the hormone experiments, it was observed that the application of 100 μM ABA and 200 μM GA yielded the most advantageous outcomes. Additionally, the most appropriate reference genes for different treatments are ACT and TUB1 (H2O treatment), EIF3 and MON1 (ABA, GA treatment and also for the combined data set of the three groups). However, GAPDH exhibited the least stability across all treatments. In summary, ACT is recommended as the reference gene for natural quinoa germination, while EIF3 and MON1 should be used for ABA and GA treatments.

show abstract

Mapping the Genomic Regions Controlling Germination Rate and Early Seedling Growth Parameters in Rice

Cited by 2 publications

References 61 publications

Transfer of Stress Resilient QTLs and Panicle Traits into the Rice Variety, Reeta through Classical and Marker-Assisted Breeding Approaches

Transfer of Stress Resilient QTLs and Panicle Traits into the Rice Variety, Reeta through Classical and Marker-Assisted Breeding Approaches

The identification of suitable internal reference genes in quinoa seeds subjected to abscisic acid and gibberellin treatment

Contact Info

Product

Resources

About