Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution

Casiraghi, Maurizio; Bain, O.; Guerrero, Ricardo; Martin, Coralie; Pocacqua, Vanessa; Gardner, Scott Lyell; Franceschi, Alberto; Bandi, Claudio

doi:10.1016/j.ijpara.2003.10.004

Cited by 242 publications

(241 citation statements)

References 54 publications

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“…Indeed, a species parasitc in North American rodents Geomyioidea, Litomosa westi (Gardner & Schmidt, 1986), which was initially placed in Litomosoides, would represent a link. This hypothesis is supported by the 12S rDNA analysis (Casiraghi et al, 2004;work in progress). Wolbachia is present in Litomosoides, with one exception (L. yutajensis Guerrero, Martin & Bain, 2003) (Xie et al, 1994; work in progress) have been performed with one species of Cutifilaria and three species from primates belonging to the subgenera Mansonella, Esslingeria Chabaud &Bain, 1976 andTetrapetalonema Faust, 1935, respectively.…”

Section: Th Emop August 2008mentioning

confidence: 64%

“…Loxodontofilaria also harbours Wolbachia of the group C, very similar to the endosymbionts harboured by members of the genus Onchocerca. Interestingly, Wolbachia is absent in the two Acanthocheilonema species studied (Casiraghi et al, 2004). According to the hypothesis proposed by Chabaud & Bain (1994), these would be close to the roots of Gondwanian Acanthocheilonema-like filariae from which stemmed Dipetalonema s. s. in the Neotropical region, when platyrhinian monkeys arrived after their transoceanic migration (late Eocene).…”

Section: Is the Dipetalomema Lineage A Reality?mentioning

confidence: 68%

“…According to the hypothesis proposed by Chabaud & Bain (1994), these would be close to the roots of Gondwanian Acanthocheilonema-like filariae from which stemmed Dipetalonema s. s. in the Neotropical region, when platyrhinian monkeys arrived after their transoceanic migration (late Eocene). D. gracile, the single species studied in this derived branch, harbours a Wolbachia related to the C supergroup, even though its real assignation is still under discussion (see below and Casiraghi et al, 2004;2005).…”

Section: Is the Dipetalomema Lineage A Reality?mentioning

confidence: 99%

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The Nematoda Filarioidea: critical analysis linking molecular and traditional approaches

Bain¹,

Galimberti

Martin³

et al. 2008

Parasite

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Summary :The molecular analysis of the Filarioidea and the endobacteria Wolbachia is no more limited to the agents of human diseases and the diversified sampling permits a synthesis with the morphological and biological results. The validity of the genera with "uncoherent host range", such as Mansonella, Litomosoides and Cercopithifilaria, is confirmed and, consequently, their evolution by host-switchings. Dirofilaria and Onchocerca, types of two subfamilies, appear more closely related than with other onchocercids. Waltonellinae from anurans and Oswaldofilariinae from reptiles have a basal position. These filariae, and some others also considered primitive, do not harbour Wolbachia. Evidence for transversal transmission of the bacteria and a second acquisition event is given with the supergroup F, identified in Mansonella, in one of the Cercopithifilaria species and in arthropods.

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Section: Th Emop August 2008mentioning

confidence: 64%

Section: Is the Dipetalomema Lineage A Reality?mentioning

confidence: 68%

Section: Is the Dipetalomema Lineage A Reality?mentioning

confidence: 99%

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The Nematoda Filarioidea: critical analysis linking molecular and traditional approaches

Bain¹,

Galimberti

Martin³

et al. 2008

Parasite

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“…Briefly, PCR was performed in a final volume of 50 µl containing 1 × Ex Taq buffer including 2 mM Mg 2+ , 200 µM each of dNTPs, 0.1 µM each of primers, 1.25 units of Ex Taq Polymerase (TAKARA BIO INC., Otsu, Japan), and 3 µl of template DNA under the following thermal conditions: an initial denaturation at 94°C for 3 min, followed by five cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min and 37 cycles of 94°C for 30 s, 48°C for 30 s, and 72°C for 1 min. The mitochondrial 12S rRNA gene of three of nine samples was also amplified with the primer set 12SF-12SR described by Casiraghi et al (2004) in the same reaction mixture as above. The thermal profile was as follows: 40 cycles of 94°C for 45 s, 50°C for 45 s, and 72°C for 90 s (Casiraghi et al, 2004).…”

Section: Regionsmentioning

confidence: 99%

“…The mitochondrial 12S rRNA gene of three of nine samples was also amplified with the primer set 12SF-12SR described by Casiraghi et al (2004) in the same reaction mixture as above. The thermal profile was as follows: 40 cycles of 94°C for 45 s, 50°C for 45 s, and 72°C for 90 s (Casiraghi et al, 2004). PCR products were purified with a QIA quick PCR Purification Kit (QIAGEN) and directly sequenced using the PCR primers, a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA), and an Applied Biosystems 3130 Genetic Analyzer (Applied Biosystems).…”

Section: Regionsmentioning

confidence: 99%

Molecular identification of infective larvae of three species ofOnchocercafound in wild-caught females ofSimulium bidentatumin Japan

et al. 2010

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Summary :Wild female black flies attracted to a man or an idling automobile were collected at Oita, Japan where five cases of zoonotic onchocerciasis had occurred. Among the five Simulium species captured, 2 % of Simulium bidentatum, the predominant species, were infected with filarial larvae. There were at least two types of infective larvae, types A and B, based on morphometric observation. Moreover, molecular analysis of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene revealed that types A and B were represented by a single unknown species of Onchocerca and two species, i.e., Onchocerca dewittei japonica from wild boar, the causative agent of zoonotic onchocerciasis in Japan, and an undescribed Onchocerca sp. from wild boar, respectively. Phylogenetic analysis based on the sequences of the mitochondrial 12S ribosomal RNA (12S rRNA) gene also showed that type A is likely to be an unknown species of Onchocerca. Natural infection of black flies with infective larvae of O. dewittei japonica and O. sp. was demonstrated for the first time. The present study strongly suggests that S. bidentatum plays a role as a vector in the transmission of zoonotic onchocerciasis due to O. dewittei japonica in Japan. Résumé : IDENTIFICATION MOLÉCULAIRE DES LARVES INFECTANTES DE TROIS ESPÈCES D'ONCHOCERQUES RÉCOLTÉES CHEZ DES FEMELLES

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Transfection ofWolbachia pipientisintoDrosophilaEmbryos

Dobson

2007

CP Microbiology

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Wolbachia is a genus of obligate intracellular Alpha‐Proteobacteria represented by the type species Wolbachia pipientis (Dumler et al., 2001). Wolbachia commonly reside within cytoplasmic vacuoles of arthropods and helminths (Werren and Windsor, 2000; Casiraghi et al., 2004); vertebrate infections have not been identified. Wolbachia are maternally transmitted from mothers to offspring though the embryonic cytoplasm. Wolbachia are able to induce a diverse range of phenotypes in their invertebrate hosts, ranging from classical mutualism to reproductive parasitism. Examples of the latter include male killing, host feminization, parthenogenesis, and cytoplasmic incompatibility (reviewed in Dobson, 2003a). Current Wolbachia research foci include examining the impacts of Wolbachia infection on host evolution, characterizing the mechanisms by which Wolbachia manipulate invertebrate hosts, and developing applied strategies that employ Wolbachia for pest and disease control. Wolbachia transfection has proven a useful technique for addressing questions within each of these research foci. This unit describes a method for Wolbachia transfection via embryonic microinjection.

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Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution

Cited by 242 publications

References 54 publications

The Nematoda Filarioidea: critical analysis linking molecular and traditional approaches

The Nematoda Filarioidea: critical analysis linking molecular and traditional approaches

Molecular identification of infective larvae of three species ofOnchocercafound in wild-caught females ofSimulium bidentatumin Japan

Transfection ofWolbachia pipientisintoDrosophilaEmbryos

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