2021
DOI: 10.1016/j.bpj.2020.11.079
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Mapping Transmembrane Binding Partners for E-Cadherin Ectodomains

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Cited by 7 publications
(15 citation statements)
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“…For this reason, we engineered the Ecad-KO cell lines and show that we are not observing any significant heterophilic interactions. For instance, while Ecad can interact heterophilically with several transmembrane proteins, including Desmoglein-2 (Dsg2) and P-cadherin (Pcad) (55,56), our control measurements with Ecad-KO cells show that there is no significant binding of Ecad with other cellsurface proteins (Fig. 2B).…”
Section: Discussionmentioning
confidence: 85%
“…For this reason, we engineered the Ecad-KO cell lines and show that we are not observing any significant heterophilic interactions. For instance, while Ecad can interact heterophilically with several transmembrane proteins, including Desmoglein-2 (Dsg2) and P-cadherin (Pcad) (55,56), our control measurements with Ecad-KO cells show that there is no significant binding of Ecad with other cellsurface proteins (Fig. 2B).…”
Section: Discussionmentioning
confidence: 85%
“…Single-molecule AFM experiments. Purified canine Ecad monomers were immobilized on AFM cantilevers (Hydra 2R-50N; AppNano) and glass coverslips (CS) as described previously (38,39). Briefly, the CS and cantilevers were cleaned with 25% H 2 O 2 /75% H 2 SO 4 overnight and washed with Milli-Q water.…”
Section: Methodsmentioning
confidence: 99%
“…In this study we report the development of a flavin-based photoredox labeling platform for proteomic analysis of protein microenvironments within cellular synaptic regions. While most intercellular labeling technologies rely on the use of enzyme-based methods with a primary focus on imaging analysis of physical cell interactions [41][42][43][44][45] , only two enzyme-based approaches (BioID and HRP) have reported proteomic profiling within cell-cell contact environments 11,12 . Whereas those two methods utilized samecell synapse forming monoculture systems, our approach enriched protein microenvironments from coculture systems consisting of synapses formed between two different cell types.…”
Section: Discussionmentioning
confidence: 99%
“…Proximity labeling strategies such as BioID 5 , APEX 6 , and TurboID 7 have been developed that rely on the use of an engineered ligase or peroxidase enzyme fused to a protein of interest to generate reactive biotin-containing species to tag neighboring protein environments. These methods have been extensively used to proteomically profile subcellular compartments [8][9][10] , as well as cell-cell interfaces 11,12 . While these approaches have been transformative in identifying protein networks within subcellular regions, the enzyme dependency of these technologies may perturb the overall physical properties of the targeting therapeutic modality as well as limit the ability for temporal control of the labeling reaction.…”
Section: Introductionmentioning
confidence: 99%