Estimating the exposure of agriculture to climate variability and change can help us understand key vulnerabilities and improve adaptive capacity, which is vital to secure and increase world food production to feed its growing population. A number of indices to estimate exposure are available in literature. However, testing or validating them is difficult and reveals a considerable variability, and no systematic methodology has been developed to guide users in selecting indices for particular applications. This need is addressed in this paper by developing a flowchart from a conceptual model that uses a system's approach. Also, we compare five approaches to estimate exposure indices (EIs) to study the exposure of agriculture to climate variability and change: single stressor-mean climate, single stressor-extreme climate, multiple stressor-mean climate, multiple stressor-extreme climate; and combinations of the above approaches. The developed flowchart requires gathering information on the region of study, including its agriculture, stressor(s), climate factor(s) (CF), period of interest and the method of aggregation. The flowchart was applied to a case study in Kansas to better understand the five approaches to estimate EIs and the implications of the choices made in each step on the estimated the exposure. The flowchart provides options that guide EI estimation by selecting the most appropriate stressor(s), associated CF(s), and aggregation methods when a detailed methodological analysis is possible, or proposes a default method when data or resources do not allow a detailed analysis. Climate adaptation involves integration of a multitude of factors across complex systems. A more standardized approach to Climatic Change (2016) 136:647-659