Marcadores moleculares asociados al veteado de la carne en bovinos Criollos uruguayos

Armstrong, E.; Peñagaricano, Francisco; Artigas, Rody; Soto, Libia; Corbi, C.; Llambí, Silvia; Rincón, Gonzalo; Postiglioni, A.

doi:10.4321/s0004-05922011000300058

Cited by 7 publications

(7 citation statements)

References 20 publications

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“…The AC-PCR method was used to analyze SNP in the 2 exon of the leptin gene (LEP), in which both alleles are amplified simultaneously, and then identified by the size of their fragments in gel electrophoresis [11][12][13]. A mixture of reagents containing 2 µl of purified DNA, 2 µl of dNTPs, 0.2 µl of Taq DNA polymerase, 2 µl of the buffer supplied with it, as well as a set of oligonucleotide primers were prepared for PCR with a given sequence:…”

Section: Methodsmentioning

confidence: 99%

Genetic parameters of milk productivity for three lactations of Holstein cattle with different genotypes of LEP gene

et al. 2020

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The research presents the findings of DNA testing of allelic polymorphism by the AC-PCR method of the LEP gene. The research was conducted among 172 Holstein cows at Integrated Agricultural Production Centre “Stud farm named after Lenin” in Atninsky district of the Republic of Tatarstan in 2017–2018. All genotypes of the LEP gene were identified through the research. Associations of the leptin gene polymorphism with dynamics of milk production during three lactations of Holstein cows were established. The best indicators for all three lactations were found in a group of animals with the TT genotype of the LEP gene. These individuals are characterized by increased milk yield, a high yield index, and tend to increase the average daily milk yield during three lactations. These individuals are characterized by increased milk yield, a high milk yield index, and tend to increase the average daily milk yield during three lactations.

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Section: Methodsmentioning

confidence: 99%

Genetic parameters of milk productivity for three lactations of Holstein cattle with different genotypes of LEP gene

et al. 2020

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“…To analyze the SNP of exon 2 of the leptin gene (LEP), the AC -PCR method was used according to the protocol developed under the guidance of E. Armstrong [14]. This system is based on the use of four primers LEP-F1 + LEP-R1 and LEP-F2 + LEP-R2, two external ones that limit amplification to the gene region where the mutation is located, and two internal ones that generate a second variable-size amplicon, depending from which allele C or T hybridized its end 3 '.…”

Section: Methodsmentioning

confidence: 99%

“…The gene encoding SCD1 consists of 6 exons and 5 introns, located on 26 bovine chromosomes [12], where some QTLs responsible for fertility, fat content, milk yield and other quantitative traits were also identified [13,14]. DNA diagnostics of polymorphism of genes associated with the same sign of milk productivity in a complex can be considered more effective than the study of each gene separately [15].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of dairy productivity of Holstein heifers depending on their genetic potential

2020

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The study presents the results obtained in DNA testing of allelic polymorphism by PCR-RFLP based methods for the leptin and stearoyl-coenzyme A desaturase genes and the interaction of their complex genotypes on the milk productivity indicators of Holstein heifers of Tatarstan population. The study was carried out among 471 Holstein cow-heifers by Integrated Agricultural Production Centre "Stud farm named after Lenin" of Atninsky district of the Republic of Tatarstan in 2016-2018. All complex LEP/SCD1 genotypes were identified through the study. The maximum frequency was observed in the combination of heterozygous genotypes LEPTCSCD1TC -149 animals (31.5 %). Milk productivity test, based on the complex genotypes LEP and SCD1, showed that a significantly high level of productivity for 305 days of lactation was 7314.4 kg, and in heifers with a complex genotype LEPTTSCD1CC was minimal (6337.4 kg) in comparison with indicators of milk yield of heifers with other complex genotypes. According to the content of the mass fraction of fat, reliably the best indicators were established in a subpopulation of animals with a complex TT/TC genotype -3.87 %. The content of the mass fraction of protein was maximal for animals with a complex CC/CC genotype.

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“…Este ganado Criollo fue utilizado en cruzamientos comerciales con las razas Aberdeen Angus, Hereford, Caracú y cebuinas, estando sus productos introducidos en el mercado cárnico uruguayo. En la actualidad se conocen dos poblaciones comerciales con introgresión de Criollo: una en Cerro Largo (661 animales) y otra en Rivera (98 animales) (Armstrong et al, 2010).…”

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Bovinos y ovinos Criollos del Uruguay: estudios y perspectivas

Armstrong¹,

Postiglioni²

2010

Agrociencia

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Los bovinos y ovinos Criollos de América descienden del ganado introducido por los conquistadores españoles y portugueses en los Siglos XVI y XVII. En Uruguay existe una reserva de bovinos Criollos y otra de ovinos Criollos en el Parque de San Miguel, Rocha, además de varias poblaciones de Criollos con diversos grados de pureza en otras partes del país. El Departamento de Genética y Mejora Animal de la Facultad de Veterinaria (UdelaR) se ha dedicado al estudio y caracterización de estos animales desde el año 1996. Se han llevado a cabo estudios morfológicos, citogenéticos y con marcadores moleculares de ADN, entre los cuales se destacan genes relacionados a producción de leche, carne, enfermedades hereditarias y marcadores de diversidad genética. Los bovinos Criollos constituyen, además, una herramienta de gran valor para el análisis de rearreglos cromosómicos y su relación con aspectos reproductivos y epigenéticos. Los estudios demuestran la gran variabilidad genética de los Criollos debido a su adaptación al medio natural y a que no fueron sometidos a selección con fines productivos. Por otro lado, cierta homogeneidad morfológica y estudios de relaciones filogenéticas con otras poblaciones apoyan su concepción como raza, en especial del bovino Criollo Uruguayo, que ha sido más estudiado. Estudios de aptitud productiva, de habilidad materna y reproducción, de resistencia a parásitos y a otros patógenos contribuirán a evaluar el potencial de los Criollos y su posible inclusión en sistemas productivos sustentables.

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Marcadores moleculares asociados al veteado de la carne en bovinos Criollos uruguayos

Cited by 7 publications

References 20 publications

Genetic parameters of milk productivity for three lactations of Holstein cattle with different genotypes of LEP gene

Genetic parameters of milk productivity for three lactations of Holstein cattle with different genotypes of LEP gene

Characteristics of dairy productivity of Holstein heifers depending on their genetic potential

Bovinos y ovinos Criollos del Uruguay: estudios y perspectivas

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