Marcadores moleculares na predição do sexo em plantas de mamoeiro

Oliveira, E. J. de; Dantas, J. L. L.; Castellen, Milene da Silva; Lima, Diego Souza de; Barbosa, Helder de Souza; Motta, Tiago Borges Nunes

doi:10.1590/s0100-204x2007001200012

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…This is the first work that investigated the segregating potential of the SCAR markers mentioned above in the Brazilian commercial papaya cultivars 'Golden' ('Solo' group) and 'Rubi' ('Formosa' group). However, a previous report evaluated some of these markers in sexing of papaya plants from other four Brazilian commercial varieties: 'Sunrise' and 'JTA' of 'Solo' group, and 'Tainung n°1' and 'Calimosa' hybrids of 'Formosa' group (Oliveira et al 2007). Any of the markers tested (SDSP, NAPF-2, SCARpm and W11) was validated for the true sex identification of these varieties, revealing the presence of false-positives andnegatives in the analyses.…”

Section: Pcr Assays With Scar Markersmentioning

confidence: 99%

“…Sex-linked DNA markers were developed by several research groups (Parasnis et al 1999(Parasnis et al , 2000Deputy et al 2002;Lemos et al 2002;Urasaki et al 2002a, b;Chaves-Bedoya and Nuñez 2007;Oliveira et al 2007;Costa et al 2011;Reddy et al 2012), and linkage maps of the papaya genome were constructed (Sondur et al 1996;Liu et al 2004;Ma et al 2004;Yu et al 2009;Wai et al 2010Wai et al , 2012Zhang et al 2010;Gschwend et al 2011;Na et al 2012). These studies play an important role in evolutionary biology researches, molecular markers and methods have been widely used for identifying the papaya sex type at an early developing stage to improve fruit production (Ming et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Through DNA analysis using polymerase chain reaction (PCR) technology, a variety of molecular markers linked to sex traits in papaya have been developed, such as: randomly amplified polymorphic DNA (RAPD) markers (Parasnis et al 2000;Lemos et al 2002;Urasaki et al 2002a;Chaves-Bedoya and Nuñez 2007;Oliveira et al 2007;Reddy et al 2012); sequence characterized amplified region (SCAR) markers, converted from RAPD (Parasnis et al 2000;Deputy et al 2002;Urasaki et al 2002a, b;Chaves-Bedoya and Nuñez 2007); simple sequence repeat (SSR) markers (Parasnis et al 1999;Costa et al 2011). These markers were tested for the discrimination of sex type in different papaya cultivars worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Early sex discrimination in Carica papaya by nuclei FISH

2015

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Carica papaya L. is a polygamous species with three sex types: male, female and hermaphrodite. However, the sex identification is only possible after flowering, by inspection of the flowers, which takes time, labor and financial resources. Molecular biology researchers have developed a number of genetic markers in an attempt to distinguish the papaya sex before reaching reproductive maturity. In the present study, we aimed to provide a molecular diagnosis of early sexing for the female and hermaphrodite plants of two commercially important Brazilian varieties of C. papaya ('Golden' and 'Rubi'). For this, it was investigated seven sequence characterized amplified region markers previously described in the literature by the polymerase chain reaction technique, and then, it was developed a probe for fluorescence in situ hybridization (FISH) protocol by using a chosen marker as probe. This molecular marker was selected based on its consistently polymorphic banding pattern, which was also demonstrated by FISH analyses: fluorescent signals in hermaphrodite nuclei isolated from leaves of both cultivars, but no detectable intensity fluorescence signal in female nuclei from both cultivars. Once the probe was constructed, this new and reliable technique, based on FISH in nuclei, could be a valuable tool for sexing the papaya seedlings with commercial application in large scale and open the possibility towards automatized diagnostic assay.

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Section: Pcr Assays With Scar Markersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early sex discrimination in Carica papaya by nuclei FISH

2015

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“…Microsatellite markers have been used for breeding and selection, for example, in studies related to the construction of genetic maps (Chen et al 2007;Eustice et al 2008), sexual differentiation (Parasnis et al 1999;Santos et al 2003;Oliveira et al 2007) and marker-assisted selection (Oliveira et al 2010a;Pinto et al 2013) as well as in several studies of genetic diversity aimed at the organization and characterization of germplasm banks (Ocampo Pérez et al 2007;Oliveira et al 2010b;Brown et al 2012), genetic diversity, migration rates, gene flow, and sex ratio of the populations in different habitats between C. papaya and wild species (Chávez-Pesqueira et al 2014). In contrast, even with the availability of the complete papaya genome ('SunUp.'…”

Section: Introductionmentioning

confidence: 99%

Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya

2015

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The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon-Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65(-13) to 4.33(-38) for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used.

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“…Não existe nenhum caráter morfológico visual que seja totalmente eficiente para distinguir o sexo do mamoeiro em plantas juvenis (CHUTTEANG et al 2007). Embora algumas pesquisas apontem a identificação precoce do sexo por meio de marcadores moleculares (DEPUTY et al 2002, OLIVEIRA et al 2007), a análise ainda não é viável economicamente, para as condições de plantio em áreas abertas. Dessa forma, a propagação vegetativa, a partir da estaquia, se torna alternativa à propagação seminífera.…”

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Miniestaquia de mamoeiro hermafrodita ‘Golden’ com indutor auxínico, em sistema semi-hidropônico

Schmildt¹,

Schmildt²,

Alexandre³

et al. 2019

Rev. Ciênc. Agrovet.

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RESUMOO mamoeiro tradicionalmente é propagado por sementes. A propagação assexuada é uma alternativa para a produção de plantas hermafroditas com o padrão de frutos exigidos pelo mercado consumidor. Sendo assim, objetivou-se estudar a propagação vegetativa em mamoeiro hermafrodita 'Golden', testando o ácido indol-3-butírico (AIB) no enraizamento de miniestacas, em sistema semi-hidropônico. O delineamento experimental utilizado foi o inteiramente casualizado. Os tratamentos consistiram de cinco concentrações de AIB (0, 3, 6, 9 e 12 mg L -1 ), com quatro repetições de seis miniestacas retiradas de plantas hermafroditas em cada parcela. Após 60 dias do cultivo, as miniestacas foram analisadas quanto à porcentagem de enraizamento, porcentagem de sobrevivência, comprimento da maior raiz, e número de folhas. A concentração em torno de 9 mg L -1 de AIB propiciou uma melhor indução ao enraizamento das miniestacas, bem como maior sobrevivência das mudas, formação de folhas e qualidade de suas raízes. Recomenda-se o uso de 9 mg L -1 de AIB no sistema semi-hidropônico de produção de mudas usando miniestacas de mamoeiro 'Golden' hermafrodita. PALAVRAS-CHAVE:Carica papaya L., ácido indol-3-butírico, miniestacas, propagação assexuada. ABSTRACTPapaya is traditionally propagated by seeds. Asexual propagation is an alternative for the production of hermaphrodite plants, with the fruit pattern required by the consumer market. The objective of this study was to study the vegetative propagation in 'Golden' hermaphrodite papaya, by testing the indole-3-butyric acid (IBA) in the rooting of mini-cuttings in a semi-hydroponic system. The experimental design was completely randomized. The treatments consisted of five concentrations of IBA (0, 3, 6, 9, and 12 mg L -1 ), with four replicates of six mini-cuttings taken from hermaphrodite plants in each plot. After 60 days of cultivation, the mini-cuttings were analyzed for rooting percentage, survival percentage, length of the largest root, and the number of leaves. The concentration around 9 mg L -1 of IBA provided a better induction to the rooting of mini-cuttings, as well as greater seedling survival, leaf formation, and root quality. The use of 9 mg L -1 of IBA in the semi-hydroponic system of seedling production is recommended using 'Golden' hermaphrodite papaya mini-cuttings.A forma de propagação do mamoeiro mais utilizada em todo o mundo é por sementes. Destaca-se que no Brasil o plantio é feito em área aberta e em alguns países da Europa, como na Espanha, em ambiente de cultivo protegido (SALINAS et al. 2017).No Brasil, as cultivares de mamoeiro plantadas comercialmente são de populações ginóicoandromonóicas, em que as plantas são femininas ou hermafroditas. Para os mercados brasileiro, europeu e americano, a preferência é por frutos oriundos de plantas hermafroditas (SCHMILDT et al. 2015), ao qual

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Marcadores moleculares na predição do sexo em plantas de mamoeiro

Cited by 12 publications

References 14 publications

Early sex discrimination in Carica papaya by nuclei FISH

Early sex discrimination in Carica papaya by nuclei FISH

Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya

Miniestaquia de mamoeiro hermafrodita ‘Golden’ com indutor auxínico, em sistema semi-hidropônico

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