MARCKS-like protein is an initiating molecule in axolotl appendage regeneration

Sugiura, Takuji; Wang, Heng; Barsacchi, Rico; Simon, András; Tanaka, Elly M.

doi:10.1038/nature16974

Cited by 96 publications

(88 citation statements)

References 27 publications

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“…We sought to re-examine these datasets using our newly acquired genomic data. Recent functional work has highlighted the role of diverged gene or protein function during regeneration [25][26][27] . Analysis of published tissue-enriched datasets 28 , combined with regeneration time courses 29,30 and our own transcriptional profiling of 22 tissues, identified five transcripts that are upregulated in the limb blastema (the mass of proliferating cells involved in regenerating the limb) with orthology limited to non-amniote vertebrates (Supplementary Information section 7).…”

Section: Species-restricted Genes In Regenerationmentioning

confidence: 99%

The axolotl genome and the evolution of key tissue formation regulators

Nowoshilow

Schloissnig

Fei

et al. 2018

Nature

Self Cite

468

478

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Salamanders serve as important tetrapod models for developmental, regeneration and evolutionary studies. An extensive molecular toolkit makes the Mexican axolotl (Ambystoma mexicanum) a key representative salamander for molecular investigations. Here we report the sequencing and assembly of the 32-gigabase-pair axolotl genome using an approach that combined long-read sequencing, optical mapping and development of a new genome assembler (MARVEL). We observed a size expansion of introns and intergenic regions, largely attributable to multiplication of long terminal repeat retroelements. We provide evidence that intron size in developmental genes is under constraint and that species-restricted genes may contribute to limb regeneration. The axolotl genome assembly does not contain the essential developmental gene Pax3. However, mutation of the axolotl Pax3 paralogue Pax7 resulted in an axolotl phenotype that was similar to those seen in Pax3 −/− and Pax7 −/− mutant mice. The axolotl genome provides a rich biological resource for developmental and evolutionary studies.

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Section: Species-restricted Genes In Regenerationmentioning

confidence: 99%

The axolotl genome and the evolution of key tissue formation regulators

Nowoshilow

Schloissnig

Fei

et al. 2018

Nature

Self Cite

468

478

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“…The identity of the thrombin‐activated protein is unknown, although some evidence suggests that it may be a potent growth factor required in very small amounts (Straube et al, 2004). Sugiura, Wang, Barsacchi, Simon, and Tanaka (2016) reported that a MARCKS (myristoylated alanine‐rich C‐kinase substrate)‐like protein called the muscle LIM protein (MLP) initiates entry into the cell cycle of muscle‐derived blastema cells. This protein clusters phylogenetically with other vertebrate MLPs, which generally play a role in muscle differentiation.…”

Section: Formation Of the Accumulation Blastemamentioning

confidence: 99%

Mechanisms of urodele limb regeneration

Stocum

2017

Regeneration

109

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This review explores the historical and current state of our knowledge about urodele limb regeneration. Topics discussed are (1) blastema formation by the proteolytic histolysis of limb tissues to release resident stem cells and mononucleate cells that undergo dedifferentiation, cell cycle entry and accumulation under the apical epidermal cap. (2) The origin, phenotypic memory, and positional memory of blastema cells. (3) The role played by macrophages in the early events of regeneration. (4) The role of neural and AEC factors and interaction between blastema cells in mitosis and distalization. (5) Models of pattern formation based on the results of axial reversal experiments, experiments on the regeneration of half and double half limbs, and experiments using retinoic acid to alter positional identity of blastema cells. (6) Possible mechanisms of distalization during normal and intercalary regeneration. (7) Is pattern formation is a self‐organizing property of the blastema or dictated by chemical signals from adjacent tissues? (8) What is the future for regenerating a human limb?

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“…For instance, this approach recently indicated functions for a novel MARCKS-like protein sufficient to activate cell proliferation during axolotl limb and tail regeneration (120). Although accessing gene function in vivo using antisense morpholinos is quick and inexpensive, their off-target effects have drawn high-profile concern (58; 100; 117).…”

Section: Identification Of Molecular Factors Required For Regeneramentioning

confidence: 99%

Regeneration Genetics

Chen

Poss

2017

Annu. Rev. Genet.

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Understanding how and why animals regenerate complex tissues has potential to transform regenerative medicine. Here we present an overview of genetic approaches that have recently been applied to dissect mechanisms of regeneration. We describe new advances that relate to central objectives of regeneration biolsts researching different tissues and species. These objectives include defining the cellular sources and key cell behaviors in regenerating tissue; elucidating molecular triggers and brakes for regeneration; and defining the earliest events that control the presence of these molecular factors.

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MARCKS-like protein is an initiating molecule in axolotl appendage regeneration

Cited by 96 publications

References 27 publications

The axolotl genome and the evolution of key tissue formation regulators

The axolotl genome and the evolution of key tissue formation regulators

Mechanisms of urodele limb regeneration

Regeneration Genetics

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