Marek's disease virus encoded miR-M6 and miR-M10 are dispensable for virus replication and pathogenesis in chickens

Yang, Shuaikang; Liao, Yifei; Zhang, Shuai; Lu, Wenlong; Jin, Jiaxin; Teng, Maikun; Chai, Shujun; Luo, Jun; Zhang, Gaiping; Sun, Aijun; Zhuang, Guoqing

doi:10.1016/j.vetmic.2021.109248

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…In MDV, LATs were more thoroughly characterized at their 5′ end, with the identification of several miRNA sequences processed from the first intron ( 39 , 67 ) and a polymorphism affecting both the LATs promoter and the first exons according to the virulence level of the MDV strain ( 46 , 68 , 69 ). Functional characterization of this region focused on viral miRNAs involved in Marek’s disease pathogenesis ( 70 – 72 ). One of these has been more deeply characterized because it was shown to target two viral genes involved in MDV productive infection and reactivation ( 39 ).…”

Section: Discussionmentioning

confidence: 99%

Marek’s Disease Virus Virulence Genes Encode Circular RNAs

Chasseur

Trozzi

Istasse

et al. 2022

J Virol

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Section: Discussionmentioning

confidence: 99%

Marek’s Disease Virus Virulence Genes Encode Circular RNAs

Chasseur

Trozzi

Istasse

et al. 2022

J Virol

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“…Animal experiments were conducted as previously described 28,29 . In brief, specific pathogen‐free (SPF) chickens (Boehringer Ingelheim, Beijing, China) were randomly sorted into experimental groups.…”

Section: Methodsmentioning

confidence: 99%

“…Animal experiments were conducted as previously described. 28,29 In brief, specific pathogen-free (SPF) chickens (Boehringer Ingelheim, Beijing, China) were randomly sorted into experimental groups. Fiveday-old chickens were inoculated subcutaneously with 2000 plaque formation units (PFU) of MDV.…”

Section: Animal Experiments Cell Culture and Virusesmentioning

confidence: 99%

Infection phase‐dependent dynamics of the viral and host N6‐methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo

Zhuang

Zhao

Jin

et al. 2022

Journal of Medical Virology

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Dynamic alteration of the epitranscriptome exerts regulatory effects on the lifecycle of oncogenic viruses in vitro. However, little is known about these effects in vivo because of the general lack of suitable animal infection models of these viruses. Using a model of rapid‐onset Marek's disease lymphoma in chickens, we investigated changes in viral and host messenger RNA (mRNA) N6‐methyladenosine (m6A) modification during Marek's disease virus (MDV) infection in vivo. We found that the expression of major epitranscriptomic proteins varies among viral infection phases, reprogramming both the viral and the host epitranscriptomes. Specifically, the methyltransferase‐like 3 (METTL3)/14 complex was suppressed during the lytic and reactivation phases of the MDV lifecycle, whereas its expression was increased during the latent phase and in MDV‐induced tumors. METTL3/14 overexpression inhibits, whereas METTL3/14 knockdown enhances, MDV gene expression and replication. These findings reveal the dynamic features of the mRNA m6A modification program during viral replication in vivo, especially in relation to key pathways involved in tumorigenesis.

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“…In vitro growth kinetics were determined for MDVs, as previously described ( 21 , 57 to 59 ). Briefly, CEFs were seeded onto 60-mm plates and inoculated with 100 PFU of each virus.…”

Section: Methodsmentioning

confidence: 99%

“…The cycle threshold (Ct) values for the chicken ovotransferrin ( OVO ) gene and the viral ICP4 gene were determined by qPCR and a standard curve equation calculated according to the linear relationship between the logarithm of genome copy number and Ct. Viral genome copy number was calculated to compare the in vitro growth kinetics and replication of the recombinant and parental viruses, as previously described ( 21 , 57 to 59 ).…”

Section: Methodsmentioning

confidence: 99%