Introduction Wax refers to the mixture of long-chain apolar compounds forming a protective layer on the surface of some plant and animal tissues, algae, fungi and bacteria. Also, there are some mineral waxes originating from fossil sources. Although chemically, a wax is the ester of a longchain acid and a long-chain alcohol, almost all waxes are a heterogeneous mixture of various compounds including hydrocarbons, wax esters, sterol esters, aldehydes, ketones, alcohols, alkanoic acids, terpenes and others. Typically waxes are soluble in oils, non-polar solvents, ethers, esters and ketones. They are kneadable at room temperature, have low viscosity above their melting point, generally having no stinginess, coarse or fine crystalline materials, having a melting range between 40 and 120 , mostly emulsion forming, combustible, lubricant, having a high flash point and high dielectric constant compounds. They could be produced with various colors and odors depending on the level of refining and/or processing conditions 1, 2. Waxes are usually classified according to their origins plant waxes, animal waxes, microbial waxes, mineral waxes, synthetic waxes, etc. , and named after their source materials. In this study, selected animal waxes beeswax, shellac wax, whale spermaceti wax, and lanolin