Margination and Emigration of Leucocytes

Colditz, Ian G.

doi:10.1159/000156964

Cited by 36 publications

(28 citation statements)

References 68 publications

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“…Interaction with endothelial cells is fundamental to the localized infiltration of neutrophils and monocytes into extravascular inflammatory sites (1,2) or the homing of lymphocytes to lymphoid organs (2)(3)(4). Recent experimental and clinical observations have begun to define the molecular determinants on the surface of leukocytes that contribute to the adhesive component ofthis interaction (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.

et al. 1988

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Human neutrophil (PMN) attachment to human umbilical vein endothelial cells (HUVEC) was evaluated in vitro using two MAbs, R6-5-D6 and RRi/1, that recognize intercellular adhesion molecule-i (ICAM-1), and one MAb, TS1/18, that recognizes CD18. Pretreatment of the HUVEC with anti-ICAM-1 MAbs produced > 50% inhibition of attachment to HUVEC, and IL-1 (0.5 U/ml)-or lipopdysaccharide (LPS) (10 ng/ml)-stimulated HUVEC, and > 99% inhibition of f-Met-Leu-Phe (0.5 nM) enhanced adherence. Anti-ICAM-i MAbs also inhibited by > 85% the transendothelial migration induced by 4-h IL-1 (0.5 U/ml) and LPS (10 ng/ml) activation of the HUVEC. That these effects involved a CD18-dependent mechanism is supported by the following results: pretreatment of PMN with TS1/18 produced the same degree of inhibition of attachment and migration as seen with R6-5-D6. In addition, the use of both MAbs together did not further increase the inhibition of cell attachment to stimulated HUVEC. The attachment of PMN from patients with CD18 deficiency to stimulated HUVEC was not reduced by R6-5-D6, and both R6-5-D6 and TSI/18 revealed the same time course for appearance and disappearance of an adherence component on stimulated HUVEC not blocked by either MAb. These results demonstrate that attachment and transendothelial migration of PMN in vitro depend substantially on both CD18 on the PMN and ICAM-1 on the endothelial cell.

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Section: Introductionmentioning

confidence: 99%

Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.

et al. 1988

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“…of the blood and of the connective tissue, which tends to eliminate the injurious agent and to repair the damaged tissue' [Movat, 1971]. The emigration of leukocytes, predominantly neutrophils, from the blood of postcapillary' and larger venules into tissues, and their ac cumulation at sites of microbial infection, immune complex deposition or various forms of nonspecific injury, represent the hallmark of an acute inflammatory reaction [Colditz, 1985;Movat, 1985]. Other vascular phenomena of inflammation, such as altera tions in the flow of blood in the microcircu lation (hyperemia, stasis), increase in vasopermeability and microhemorrhage arc im portant components of the inflammatory reaction.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Emigration and Microvascular Injury

Movat

Cybulsky²

1987

Path Immunopathol Res

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“…To encompass the known phenomena a general theory of leukocyte margination and migration would predict that leukocytes selectively marginate on the acceptor molecules expressed by endothelium and extravasate in response to a chemotactic stimulus (Colditz, 1985). During lymphocyte recirculation high endothelial venules represent a primary site for lymphocyte migration to lymph nodes, tonsils mucosa associated lymphoid tissues and to connective tissues under inflammatory conditions (Dag˘deviren et al).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration

Deveci

2010

Int. J. Morphol.

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SUMMARY:Leukocyte-endothelial cell interactions have a pivotal role in immune responses. In our study Ten adult wistar albino rats weighing 180-200 g were used to obtain tissue samples. The animals were sacrificed by decapitation under ether anesthesia. The lymph nodes of the animals were then quickly removed. Specimens were immersed in the zinc iodide-osmium tetroxide (ZIO) solutation by Niebauer et al. (1969) and kept in the dark for 2 hours in this solution from temperature for fixation/staining. Samples were processed according to routine plastic embedding tecnique. Semithin sections of 1µm thick were cut and stained with methilene blueazure II for light microscopic examination. We used zinc iodide-osmium tetroxide staining tecnique to distinguish endothelial cell from leukocytes.Present data supports the understanding of this unique relationship as documented by figures.

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Margination and Emigration of Leucocytes

Cited by 36 publications

References 68 publications

Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.

Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.

Neutrophil Emigration and Microvascular Injury

Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration

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