Maribacter algicola sp. nov., isolated from a marine red alga, Porphyridium marinum, and transfer of Maripseudobacter aurantiacus Chen et al. 2017 to the genus Maribacter as Maribacter aurantiacus comb. nov.

Abstract

A Gram-stain-negative, strictly aerobic and moderately halophilic bacterial strain, designated strain PoM-212T, was isolated from a marine alga, Porphyridium marinum, in the Republic of Korea. Cells were catalase- and oxidase-positive rods with gliding motility without flagellum. Growth was observed at 20–40 °C (optimum, 30 °C) and pH 5.0–8.5 (pH 5.0–5.5) and in the presence of 0.5–5 % (w/v) NaCl (2–3 %). Strain PoM-212T contained iso-C15 : 1, iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids and menaquinone-6 as the sole isoprenoid quinone. Phosphatidylethanolamine, three unidentified aminolipids and two unidentified polar lipids were detected as major polar lipids. The G+C content of the genomic DNA was 41.0 mol%. Strain PoM-212T was most closely related to Maribacter lutimaris KJ4T, Maripseudobacter aurantiacus CDA4T and Maribacter flavus C3T with 97.2, 97.2 and 97.0 % 16S rRNA gene sequence similarities, respectively, and it formed a distinct phylogenetic lineage from them within the genus Maribacter . Maripseudobacter aurantiacus formed a close phylogenetic lineage with Maribacter flavus with 99.7 % 16S rRNA gene sequence similarity, but their digital DNA–DNA hybridization value was 67.4 %. On the basis of its phenotypic, chemotaxonomic and molecular properties, strain PoM-212T represents a novel species of the genus Maribacter , for which the name Maribacter algicola sp. nov. is proposed. The type strain is PoM-212T (=KACC 19789T=JCM 32941T). The transfer of Maripseudobacter aurantiacus to the genus Maribacter as Maribacter aurantiacus comb. nov. is also proposed (type strain CDA4T=KCTC 52409T=MCCC 1K03210T).

Octadecabacter algicola sp. nov. and Octadecabacter dasysiphoniae sp. nov., isolated from a marine red alga and emended description of the genus Octadecabacter

“…Members of the genus Octadecabacter have a very wide range of genome size (3.26–5.48 Mb) with 54.0–57.0 % DNA G+C contents. During our research regarding the interactions between marine red algae and bacteria, we have isolated many novel bacteria from marine red algae [5–7]. In this study, we isolated two presumably novel species of the genus Octadecabacter from a marine red alga and characterized their taxonomic properties using a polyphasic approach.…”

“…Strains D2-3 T and G9-8 T were isolated from a marine red alga, a species of the genus Dasysiphonia , collected from the East Sea (38°50′44″N, 128°42′57″E and 38°35′69″N 128°51′5″ E) off the Republic of Korea, as described previously [6] with some modifications. In brief, the collected marine red alga was washed by agitating in sterilized artificial seawater (ASW; 20 g NaCl, 2.9 g MgSO 4 , 4.53 g MgCl 2 ‧6H 2 O, 0.64 g KCl, and 1.75 g CaCl 2 ‧2H 2 O per litre), and the washed red alga was mechanically homogenized using a T10 basic homogenizer (IKA) for 1 min and serially diluted in ASW.…”

Tenacibaculum aquimarinum sp. nov., isolated from a marine alga and seawater

“…The whole genomes of strains K20-16 T and MSW2 were sequenced using an Illumina platform and Oxford Nanopore technology, respectively, as described previously [12]. The genomic DNA of strain K20-16 T was extracted using a Wizard Genomic DNA purification kit (Promega) according to the procedure of the manufacturer and sequenced using a Hiseq X instrument (Illumina) with 151 bp paired-end reads at Macrogen (Seoul, Republic of Korea), and the resulting Illumina sequencing reads were de novo -assembled using SPAdes (version 3.11.1) [16].…”

“…Strains K20-16 T and MSW2 were isolated from a marine red alga ( Chondrus species) and seawater that were collected from the East Sea (38° 21′ 23.8″ N 128° 30′ 47.3″ E) and Yellow Sea (37° 23′ 42″ N 126°44′ 19″ E) of the Republic of Korea, respectively, as described previously with some modifications [12]. For the isolation of strain K20-16 T , the collected marine red alga was washed using sterilized artificial seawater (ASW; 20 g l −1 NaCl, 2.9 g l −1 MgSO 4 , 4.53 g l −1 MgCl 2 ‧6H 2 O, 0.64 g l −1 KCl and 1.75 g l −1 CaCl 2 ‧2H 2 O).…”

Nitratireductor rhodophyticola sp. nov., isolated from marine red algae

“…Cells of Nitratireductor members are generally strictly aerobic, Gramnegative, catalase-and oxidase-positive and motile or non-motile rods containing ubiquinone-10 (Q-10) as the major respiratory quinone and C 19 : 0 cyclo ω8c and summed feature 8 (comprising C 18 : 1 ω7c and/or C 18 : 1 ω6c) as the major fatty acids, and they have 56.7-65.6 mol% G+C contents [4,5]. During research regarding the interactions between marine red algae and bacteria, we have isolated many novel bacteria from marine red algae [8][9][10]. In this study, we isolated two strains belonging to the genus Nitratireductor, designated strains L1-7-SE T and R6, from marine red algae, Porphyridium marinum and Rhodosorus marinus, respectively and analysed their taxonomic characteristics using a polyphasic approach.…”