Maricaulis alexandrii sp. nov., a novel active bioflocculants-bearing and dimorphic prosthecate bacterium isolated from marine phycosphere

Xiao-ling, Zhang; Qi, Min; Li, Qiuhong; Cui, Zhendong

doi:10.1007/s10482-021-01588-6

Cited by 11 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For nitrogen metabolism, 10 genomes of M. alba strains also harbored genes encoding for dissimilatory nitrate reductase ( nar G, nar H, nar I, nar J, nar K), and ure A, ure B, ure C, ure D, ure E, ure F, ure G encoding for urease showing the urea assimilation by the M. alba strains. The DMSP and amino acids provided by phycosphere microbiota will benefit the algal host’s nutrition acquirement during algae-bacteria interactions [34–37]. Moreover, stress response systems to defense oxidative stress were found in 10 genomes of M. alba strains.…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics reveals insights into phylogenomic taxonomy and potential algae-bacteria interactions of novel versatile Mameliella alba strain LZ-28 isolated from highly-toxic marine phycosphere

Zhu

Fang²,

Y³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Phycosphere harbors cross-kingdom interactions with significant ecological relevance for harmful algal blooms (HAB) and phycotoxins biosynthesis. Previously, a new red-pigmented bacterium designated as strain LZ-28 was isolated from phycosphere microbiota of typical HAB dinoflagellate Alexandrium catenella LZT09 which is a vitamin B 12 auxotroph and produces high levels of paralytic shellfish poisoning toxins (PST). Strain LZ-28 exhibited obvious growth-promoting activity toward its algal host, along with the production of active bioflocculanting exopolysaccharides (EPS). But the phylogenetic affiliation and genomic potential of this versatile bacterium has not yet been elucidated. In this study, we carried out combined taxonomic and phylogenomic analysis to clarify the taxonomic classification of strain LZ-28. The obtained 16S rRNA phylogeny revealed close taxonomic relationship between strain LZ-28 and other Mameliella alba members. Additional calculations of key phylogenomic parameters, average nucleotide identity (ANI), the average amino acid identity (AAI) and the digital DNA-DNA hybridization (dDDH) values based on genomes of strain LZ-28 and type strain of Mameliella alba were all exceeded the limit of species circumscription. Collectively considering the phenotypic and biochemical characterizations, strain LZ-28 was therefore identified as a new member of Mameliella alba. Furthermore, based on the genomic evidence, potential algae-bacteria interactions of strain LZ-28 with host algae LZT09 were elucidated through the associations with photosynthetic and antioxidant carotenoids, supplying of bacterial VB 12 to auxotroph host, and versatile EPS serving for bacterial colonization and nutrient exchange during their interactions, along with stress response systems to defense oxidative stress and quorum sensing (QS) signals benefited survival for bacteria in the symbiotic system. Comparative genomics shed light on similar genomic features between M . alba strains, revealed potential close associations of strain LZ-28 with its algae host, and further enriched the genomic repertoire of interactions between phycosphere microbiota and algal host LZT09.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative genomics reveals insights into phylogenomic taxonomy and potential algae-bacteria interactions of novel versatile Mameliella alba strain LZ-28 isolated from highly-toxic marine phycosphere

Zhu

Fang²,

Y³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…It can be clearly seen that the bioflocculanting activity reached the maximum of 96.7 ± 1.8% (means ± SD) at the EPS concentration of 0.60 g•L −1 . It exhibited a higher bioflocculanting capacity compared with other marine bacterial strains isolated from marine phycosphere niches that we previously reported [29][30][31]. These findings obviously indicate that strain NJES-13 may serve as a novel actinobacterial candidate with natural potential for the production of promising microbial bioflocculants derived from the unique mammalian gut microbiota niche in the antarctic habitat.…”

Section: Bioflocculanting Potential Of Bacterial Epsmentioning

confidence: 56%

“…Initially, the transmission electron microscope observation of strain NJES-13 cells clearly demonstrated the presence of an EPS layer outside of the cells (Figure 1). Then, the extracted EPS produced by strain NJES-13 were subjected to the bioflocculanting activity screening assay to evaluate their production capacity of bacterial bioflocculants [29][30][31]. The comparison of bioflocculanting effects under a series of EPS concentrations was performed.…”

Section: Bioflocculanting Potential Of Bacterial Epsmentioning

confidence: 99%

Isolation, Phylogenetic and Gephyromycin Metabolites Characterization of New Exopolysaccharides-Bearing Antarctic Actinobacterium from Feces of Emperor Penguin

2021

Self Cite

View full text Add to dashboard Cite

A new versatile actinobacterium designated as strain NJES-13 was isolated from the feces of the Antarctic emperor penguin. This new isolate was found to produce two active gephyromycin analogues and bioflocculanting exopolysaccharides (EPS) metabolites. Phylogenetic analysis based on pairwise comparison of 16S rRNA gene sequences showed that strain NJES-13 was closely related to Mobilicoccus pelagius Aji5-31T with a gene similarity of 95.9%, which was lower than the threshold value (98.65%) for novel species delineation. Additional phylogenomic calculations of the average nucleotide identity (ANI, 75.9–79.1%), average amino acid identity (AAI, 52.4–66.9%) and digital DNA–DNA hybridization (dDDH, 18.6–21.9%), along with the constructed phylogenomic tree based on the up-to-date bacterial core gene (UBCG) set from the bacterial genomes, unequivocally separated strain NJES-13 from its close relatives within the family Dermatophilaceae. Hence, it clearly indicated that strain NJES-13 represented a putative new actinobacterial species isolated from the gut microbiota of mammals inhabiting the Antarctic. The obtained complete genome of strain NJES-13 consisted of a circular 3.45 Mb chromosome with a DNA G+C content of 67.0 mol%. Furthering genome mining of strain NJES-13 showed the presence of five biosynthetic gene clusters (BGCs) including one type III PKS responsible for the biosynthesis of the core of gephyromycins, and a series of genes encoding for bacterial EPS biosynthesis. Thus, based on the combined phylogenetic and active metabolites characterization presented in this study, we confidently conclude that strain NJES-13 is a novel, fresh actinobacterial candidate to produce active gephyromycins and microbial bioflocculanting EPS, with potential pharmaceutical, environmental and biotechnological implications.

show abstract

“…For the actinomycete, cellular morphology is regarded as one key aspect in understanding the secondary metabolism in filamentous groups [19][20][21]. Based on ultra-structural observations of the single and aggregated cells of strain NJES-13 T using a transmission electron microscope (TEM), strain NJES-13 T formed rod-shaped cells with the filaments, surrounded by a slimy outside extracellular layer mainly composed of bacteria-derived macromolecular exopolysaccharides (EPSs) (Figure 1A,B), which is regarded as the main essential chemical basis for host-bacteria interactions [16][17][18]22]. Moreover, based on scanning electron microscope (SEM) observations, the cells of strain NJES-13 T were found to be clustered and interconnected by viscous EPSs showing an unusual three-dimensional and net-like morphology (Figure 1C).…”

Section: Fermentation Based On Glass Bead-enhanced Cultivation Of Strain Njes-13 Tmentioning

confidence: 99%

“…Recently, we reported the isolation of a novel actinobacterium strain, NJES-13 T , from the gut microbiota of the Antarctic emperor penguin Aptenodytes forsteri [12]. The new bacterial isolate was found to produce two known angucyclinone derivatives, named gephyromycin (GPM) and 2-hydroxy-tetrangomycin (2-HT), as well as complex active bioflocculation exopolysaccharide (EPS) metabolites [16][17][18]. Moreover, one extra potential new angucycline derivative was also detected, although its chemical structure was not elucidated due to its native low production level [11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bioactivities and Biosynthesis of Angucycline/Angucyclinone Derivatives Derived from Gephyromycinifex aptenodytis gen. nov., sp. nov.

et al. 2021

Self Cite

View full text Add to dashboard Cite

Strain NJES-13T is the type strain and currently the only species of the newly established actinobacteria genera Aptenodytes in the family Dermatophilaceae isolated from the gut microbiota of the Antarctic emperor penguin. This strain demonstrated excellent bioflocculation activity with bacteria-derived exopolysaccharides (EPSs). Moreover, it produced bioactive angucycline/angucyclinone derivatives (ADs) and contained one type III polyketide synthase (T3PKS), thus demonstrating great potential to produce novel bioactive compounds. However, the low productivity of the potential new AD metabolite was the main obstacle for its chemical structure elucidation. In this study, to increase the concentration of targeted metabolites, the influence of cellular morphology on AD metabolism in strain NJES-13T was determined using glass bead-enhanced fermentation. Based on the cellular ultra-structural observation driven by bacterial EPSs, and quantitative analysis of the targeted metabolites, the successful increasing of the productivity of three AD metabolites was achieved. Afterward, a new frigocyclinone analogue was isolated and then identified as 2-hydroxy-frigocyclinone, as well as two other known ADs named 2-hydroxy-tetrangomycin (2-HT) and gephyromycin (GPM). Three AD metabolites were found to demonstrate different bioactivities. Both C-2 hydroxyl substitutes, 2-hydroxy-tetrangomycin and 2-hydroxy-frigocyclinone, exhibited variable inhibitory activities against Staphylococcus aureus, Bacillus subtilis and Candida albicans. Moreover, the newly identified 2-hydroxy-frigocyclinone also showed significant cytotoxicity against three tested human-derived cancerous cell lines (HL-60, Bel-7402 and A549), with all obtained IC50 values less than 10 µM. Based on the genetic analysis after genomic mining, the plausible biogenetic pathway of the three bioactive ADs in strain NJES-13T was also proposed.

show abstract

Maricaulis alexandrii sp. nov., a novel active bioflocculants-bearing and dimorphic prosthecate bacterium isolated from marine phycosphere

Cited by 11 publications

References 22 publications

Comparative genomics reveals insights into phylogenomic taxonomy and potential algae-bacteria interactions of novel versatile Mameliella alba strain LZ-28 isolated from highly-toxic marine phycosphere

Comparative genomics reveals insights into phylogenomic taxonomy and potential algae-bacteria interactions of novel versatile Mameliella alba strain LZ-28 isolated from highly-toxic marine phycosphere

Isolation, Phylogenetic and Gephyromycin Metabolites Characterization of New Exopolysaccharides-Bearing Antarctic Actinobacterium from Feces of Emperor Penguin

Characterization of Bioactivities and Biosynthesis of Angucycline/Angucyclinone Derivatives Derived from Gephyromycinifex aptenodytis gen. nov., sp. nov.

Contact Info

Product

Resources

About